Detailed view for MUL_3503

Basic information

TDR Targets ID: 953735
Mycobacterium ulcerans, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Source Database / ID:  KEGG  

pI: 4.7982 | Length (AA): 488 | MW (Da): 50163 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02875   Mur ligase family, glutamate ligase domain
PF08245   Mur ligase middle domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0016874   ligase activity  
GO:0008764   UDP-N-acetylmuramoylalanine-D-glutamate ligase activity  
GO:0005524   ATP binding  
GO:0051301   cell division  
GO:0009058   biosynthetic process  
GO:0008360   regulation of cell shape  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 486 3lk7 (A) 7 448 29.00 0 1 1.18246 0.37
8 56 5a9t (A) 5 53 33.00 0.44 0.8 0.54821 -0.74
189 347 1w78 (A) 162 311 34.00 0.45 0.37 0.42862 1.3

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_132024)

Species Accession Gene Product
Chlamydia trachomatis CT_758   UDP-N-acetylmuramoylalanine--D-glutamate ligase
Escherichia coli b0088   UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase
Mycobacterium leprae ML0912   Probable UDP-N-acetylmuramoylalanine-D-glutamate ligase MurD
Mycobacterium tuberculosis Rv2155c   Probable UDP-N-acetylmuramoylalanine-D-glutamate ligase MurD
Mycobacterium ulcerans MUL_3503   UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Treponema pallidum TP0903   UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD)
Wolbachia endosymbiont of Brugia malayi Wbm0508   UDP-N-acetylmuramoylalanine-D-glutamate ligase

Essentiality

MUL_3503 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b0088 Escherichia coli essential goodall
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Staphylococcus aureus UDP-N-acetylmuramoylalanine-D-glutamate ligase Compounds References
Escherichia coli UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.3229 0.7184 0.7184
0.3521 0.7823 0.771
0.1436 0.2934 0.2687
0.045 0.5401 0.7907
0.3101 0.8172 0.8171
0.0416 0.4459 0.4459
0.3541 0.7684 0.7104
0.0251 0.3091 1
0.0418 0.7815 0.7692
0.1995 0.2938 0.2936
0.0251 0.3091 1
0.3521 0.7823 0.771
0.0236 0.2856 0.2903
0.394 1 1
0.1975 0.2934 0.2934
0.1922 0.2918 0.2912
0.1971 0.294 0.294
0.3752 0.8023 0.8022
0.1981 0.2944 0.294
0.1266 0.2919 0.2904
0.394 1 1
0.1658 0.2947 0.294
0.3069 0.5683 0.9291
0.1966 0.2935 0.2917
0.4123 0.8136 0.8136
0.0545 0.2927 0.2901
0.1883 0.2936 0.2936
0.2004 0.2939 0.2939
0.3752 0.8023 0.8022
0.1814 0.2936 0.2935
0.1995 0.2938 0.2936
0.0512 0.6918 0.7828
0.1947 0.2946 0.2911
0.1922 0.2936 0.2935
0.1958 0.2935 0.2917
0.394 1 1
0.401 0.8259 0.8259
0.394 1 1
0.053 0.2902 0.2191
0.394 1 1
0.3485 0.8963 0.8728
0.1959 0.2935 0.2917
0.0512 0.5336 0.6562
0.2308 0.6674 0.619
0.2089 0.2941 0.2941
0.1922 0.2918 0.2912
0.0713 0.2919 0.2904
0.1995 0.2938 0.2936
0.0512 0.3746 0.6297
0.2978 0.9237 0.9237
0.2441 0.373 0.3721
0.394 1 1
0.394 1 1
0.1981 0.2944 0.294
0.394 1 1
0.0793 0.2922 0.2922
0.3069 0.5683 0.9291
0.0414 0.2899 0.2881
0.2441 0.373 0.3721
0.2058 0.2938 0.2937
0.3541 0.7684 0.7104
0.1815 0.2941 0.2918
0.1319 0.5612 0.5399
0.268 0.8121 0.8076
0.192 0.2933 0.2915
0.2004 0.2939 0.2939
0.1971 0.2934 0.2916
0.0654 0.2682 0.2849
0.3229 0.7184 0.7184
0.1274 1 1
0.0512 0.6926 0.6868
0.1974 0.4671 0.4623
0.2099 0.2938 0.2938
0.1737 0.2913 0.291
0.1365 0.2926 0.29
0.1793 0.2932 0.2911
0.1804 0.2936 0.2935
0.2441 0.373 0.3721
0.1976 0.2945 0.2935
0.1679 0.2875 0.2189
0.1658 0.2947 0.294
0.394 1 1
0.3541 0.7684 0.7104
0.1658 0.2947 0.294
0.3069 0.5683 0.9291
0.191 0.2918 0.2189
0.0788 0.2933 0.2915
0.2441 0.373 0.3721
0.192 0.2933 0.2915
0.2013 0.2925 0.2915
0.1998 0.2942 0.294
0.0641 0.2941 0.2932
0.0392 0.2539 0.2887
0.0416 0.4089 1
0.3752 0.8023 0.8022
0.1812 0.2934 0.2934
0.394 1 1
0.1939 0.2935 0.2916
0.0512 0.6531 0.6255
0.1975 0.2934 0.2934
0.3544 0.9015 0.8947
0.0637 0.2897 0.2897
0.394 1 1
0.1995 0.2938 0.2936
0.394 1 1
0.2684 0.8269 0.8268
0.0392 0.2539 0.2887
0.1883 0.2936 0.2936
0.0418 0.7815 0.7692
0.1791 0.2935 0.2934
0.2089 0.2941 0.2941
0.3541 0.7684 0.7104
0.0416 0.481 1
0.1947 0.2946 0.2911
0.1995 0.2938 0.2936
0.1977 0.2937 0.2936
0.1192 0.2925 0.2923
0.3453 0.8827 0.8717
0.191 0.2918 0.2189
0.1669 0.2875 0.2189
0.083 0.2881 0.2489
0.1436 0.2934 0.2687
0.1815 0.2941 0.2918
0.0713 0.2919 0.2904
0.394 1 1
0.2733 0.6053 0.6053
0.0512 0.6926 0.6868
0.394 1 1
0.1136 0.2925 0.2923
0.1115 0.6577 0.5956
0.0416 0.3559 0.3559
0.095 0.2942 0.2942
0.0512 0.5286 0.5286
0.1818 0.2927 0.2927
0.3101 0.8172 0.8171
0.1998 0.2942 0.294
0.1995 0.2938 0.2936
0.3541 0.7684 0.7104
0.0416 0.5589 0.5589
0.2684 0.8269 0.8268
0.3069 0.5683 0.9291
0.208 0.2936 0.2936
0.1922 0.2918 0.2912
0.208 0.2936 0.2936
0.0416 0.4459 0.4459
0.0793 0.2922 0.2922
0.394 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier MUL_3503 (Mycobacterium ulcerans), UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
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