Detailed view for TGME49_209960

Basic information

TDR Targets ID: 263936
Toxoplasma gondii, glycosyltransferase
Source Database / ID:  ToxoDB 

pI: 6.1051 | Length (AA): 1707 | MW (Da): 188848 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00128   Alpha amylase, catalytic domain
PF00534   Glycosyl transferases group 1
PF02806   Alpha amylase, C-terminal all-beta domain
PF02922   Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
PF13439   Glycosyltransferase Family 4

Gene Ontology

Mouse over links to read term descriptions.
GO:0004553   hydrolase activity, hydrolyzing O-glycosyl compounds  
GO:0043169   cation binding  
GO:0003824   catalytic activity  
GO:0009058   biosynthetic process  
GO:0005975   carbohydrate metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1056 1438 2bis (A) 1 425 32.00 0 1 0.5 -0.31
1268 1406 2f9f (A) 16 152 31.00 0.0000000084 1 0.6 -1.64
562 1161 4bzy (A) 45 599 32.00 0 1 0.575394 0.28
759 919 2wsk (A) 153 336 28.00 0.0023 1 0.372118 -0.47
872 938 3o0w (A) 27 84 45.00 0.35 0.08 0.12105 1.77
960 1438 3s28 (A) 213 766 15.00 0 0.91 0.0915093 0.92
1055 1440 3c48 (A) 0 404 26.00 0 1 0.583028 -0.51
1209 1438 4hln (A) 334 601 33.00 0.00000000014 1 0.370539 -0.25
1268 1406 2f9f (A) 16 152 31.00 0.000000017 1 0.545229 -1.13
1271 1438 4x7r (A) 324 491 26.00 0.0000037 1 0.507218 -0.78

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127579)

Species Accession Gene Product
Arabidopsis thaliana AT2G36390   1,4-alpha-glucan branching enzyme 2-1
Arabidopsis thaliana AT5G03650   1,4-alpha-glucan branching enzyme
Brugia malayi Bm1_02775   1,4-alpha-glucan branching enzyme
Candida albicans CaO19.13067   likely glycogen branching enzyme
Candida albicans CaO19.5622   likely glycogen branching enzyme
Caenorhabditis elegans CELE_T04A8.7   Protein T04A8.7, isoform B
Cryptosporidium hominis Chro.60381   1,4-alpha-glucan branching enzyme
Cryptosporidium parvum cgd6_3280   glycogen branching enzyme (1,4-alpha-glucan branching enzyme)
Chlamydia trachomatis CT_866   1,4-alpha-glucan branching enzyme
Dictyostelium discoideum DDB_G0274105   1,4-alpha-glucan branching enzyme
Drosophila melanogaster Dmel_CG33138   1,4-Alpha-Glucan Branching Enzyme
Escherichia coli b3432   1,4-alpha-glucan branching enzyme
Echinococcus granulosus EgrG_000239850   glucan 14 alpha branching enzyme 1
Entamoeba histolytica EHI_106090   1,4-alpha-glucan branching enzyme, putative
Entamoeba histolytica EHI_038160   1,4-alpha-glucan branching enzyme, putative
Echinococcus multilocularis EmuJ_000239850   glucan (1,4 alpha), branching enzyme 1
Giardia lamblia GL50803_15823   1,4-alpha-glucan branching enzyme
Homo sapiens ENSG00000114480   glucan (1,4-alpha-), branching enzyme 1
Loa Loa (eye worm) LOAG_06995   hypothetical protein
Mus musculus ENSMUSG00000022707   glucan (1,4-alpha-), branching enzyme 1
Mycobacterium tuberculosis Rv1326c   1,4-alpha-glucan branching enzyme GlgB (glycogen branching enzyme)
Mycobacterium ulcerans MUL_3936   glycogen branching protein
Neospora caninum NCLIV_058980   1,4-alpha-glucan branching enzyme, putative
Neospora caninum NCLIV_004200   hypothetical protein
Oryza sativa 4329532   Os02g0528200
Oryza sativa 4342117   Os06g0726400
Saccharomyces cerevisiae YEL011W   1,4-alpha-glucan branching enzyme
Schistosoma japonicum Sjp_0065120   ko:K00700 1,4-alpha-glucan branching enzyme [EC2.4.1.18], putative
Schistosoma mansoni Smp_094880   starch branching enzyme II
Schmidtea mediterranea mk4.001372.07   1,4-alpha-glucan-branching enzyme
Schmidtea mediterranea mk4.001372.05  
Schmidtea mediterranea mk4.001372.06  
Schmidtea mediterranea mk4.001372.08   1,4-alpha-glucan-branching enzyme
Schmidtea mediterranea mk4.011524.03   1,4-alpha-glucan-branching enzyme
Schmidtea mediterranea mk4.005975.02   1,4-alpha-glucan-branching enzyme
Toxoplasma gondii TGME49_209960   glycosyltransferase
Toxoplasma gondii TGME49_316520   1,4-alpha-glucan-branching enzyme
Trichomonas vaginalis TVAG_453180   amylase, putative
Trichomonas vaginalis TVAG_276310   starch branching enzyme II, putative

Essentiality

TGME49_209960 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1349 Mycobacterium tuberculosis essential nmpdr
b3432 Escherichia coli non-essential goodall
CELE_T04A8.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_T04A8.7 Caenorhabditis elegans slow growth wormbase
TGME49_316520 Toxoplasma gondii Essentiality uncertain sidik
TGME49_209960 this record Toxoplasma gondii Essentiality uncertain sidik
TGME49_316520 Toxoplasma gondii Probably non-essential sidik
TGME49_209960 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Mycobacterium tuberculosis Glycosyltransferase MshA 480 aa 29.2% 415 aa Compounds References
Mycobacterium leprae GLYCOSYLTRANSFERASE MSHA 428 aa 28.6% 378 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0097 0.3881 1
0.0314 0.3687 1
0.0288 0.3758 1
0.0371 0.3991 1
0.0364 0.4199 1
0.0105 0.2881 0.5267
0.0364 0.4199 1
0.0105 0.2881 0.5267
0.0057 0.3408 1
0.0255 0.2699 1
0.0049 0.3789 1
0.0442 0.4398 1
0.027 0.3584 1
0.0366 0.2605 0.5
0.0366 0.4149 1
0.0105 0.2983 1
0.0315 0.3953 1
0.0272 0.2947 1
0.0049 0.3789 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_209960 (Toxoplasma gondii), glycosyltransferase
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