Detailed view for TP0341

Basic information

TDR Targets ID: 596349
Treponema pallidum, UDP-N-acetylmuramate--L-alanine ligase
Source Database / ID:  KEGG  

pI: 6.3219 | Length (AA): 481 | MW (Da): 53762 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01225   Mur ligase family, catalytic domain
PF02875   Mur ligase family, glutamate ligase domain
PF08245   Mur ligase middle domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016874   ligase activity  
GO:0008763   UDP-N-acetylmuramate-L-alanine ligase activity  
GO:0005524   ATP binding  
GO:0009252   peptidoglycan biosynthetic process  
GO:0009058   biosynthetic process  

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 481 2xja (D) 28 522 20.00 0 1 1.02553 0.89
14 473 4hv4 (A) 21 475 30.00 0 1 1.31334 -0.36
15 473 4hv4 (A) 22 475 31.00 0 1 1.31246 -0.25
63 158 4h1x (A) 173 266 35.00 0.021 0.55 0.410784 0.57
334 473 3mvn (A) 317 447 28.00 0 0.99 0.49906 -0.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_132146)

Species Accession Gene Product
Chlamydia trachomatis CT_762   bifunctional UDP-N-acetylmuramate-alanine ligase/D-alanine-D-alanine ligase
Escherichia coli b0091   UDP-N-acetylmuramate:L-alanine ligase
Mycobacterium leprae ML0915   Probable UDP-N-acetylmuramate-alanine ligase MurC
Mycobacterium tuberculosis Rv2152c   Probable UDP-N-acetylmuramate-alanine ligase MurC
Mycobacterium ulcerans MUL_3500   UDP-N-acetylmuramate--L-alanine ligase
Schmidtea mediterranea mk4.072324.01  
Treponema pallidum TP0341   UDP-N-acetylmuramate--L-alanine ligase
Wolbachia endosymbiont of Brugia malayi Wbm0118   UDP-N-acetylmuramate-alanine ligase

Essentiality

TP0341 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b0091 Escherichia coli essential goodall
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Staphylococcus aureus UDP-N-acetylmuramate--L-alanine ligase Compounds References
Escherichia coli UDP-N-acetylmuramate:L-alanine ligase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.1 0.4965 0.4025
0.3815 0.5785 0.5785
0.2378 0.5953 0.4076
0.1079 0.3345 0.5746
0.047242 0.480706 0.480706
0.4094 0.5782 0.4025
0.1248 0.5756 0.4025
0.0188 0.3106 0.4025
0.2385 0.4727 0.3407
0.1761 0.5403 0.5395
0.22 0.5653 0.5638
0.3864 0.5782 0.4025
0.4094 0.5782 0.4025
0.3837 0.5779 0.4025
0.4641 0.7131 0.713
0.1544 0.4779 0.4779
0.0355 0.4025 0.4025
0.0349 0.4603 0.5657
0.4641 0.7131 0.713
0.3562 0.5774 0.4025
0.325 0.5786 0.4025
0.2192 0.7787 0.7217
0.2225 0.5773 0.4025
0.2517 0.6373 0.6352
0.2024 0.5088 0.2967
0.0349 0.4552 0.5657
0.1038 0.5759 0.4025
0.1853 0.6593 0.6402
0.0677 0.563 0.563
0.3928 0.5781 0.4025
0.4641 0.7131 0.713
0.1282 0.5303 0.4025
0.0305 0.4025 0.4025
0.1726 0.7993 0.7572
0.3558 0.5782 0.5782
0.0324 0.385 1
0.0722 0.5333 0.4025
0.0404 0.563 0.563
0.0644 0.5333 0.4025
0.0188 0.5397 0.4025
0.2194 0.5778 0.5774
0.0884 0.2977 0
0.0188 0.4512 0.4025
0.1397 0.5769 0.4025
0.1834 0.2882 0.2882
0.3883 0.5784 0.4025
0.2385 0.4727 0.3407
0.22 0.5653 0.5638
0.2517 0.6373 0.6352
0.2517 0.6373 0.6352
0.2194 0.5778 0.5774
0.2541 0.6237 0.6237
0.0404 0.4869 0.4545
0.0354 0.533 0.4025
0.22 0.5653 0.5638
0.0532 0.6797 0.5602
0.3359 0.7402 0.6821
0.2335 0.5773 0.4025
0.1757 0.8024 0.8018
0.0434 0.8149 0.7347
0.3799 0.5779 0.4025
0.1761 0.5403 0.5395
0.1864 0.5333 0.4025
0.2373 0.5055 0.5053
0.3214 0.5333 0.4025
0.22 0.5653 0.5638
0.22 0.5653 0.5638
0.2517 0.6373 0.6352
0.3839 0.5779 0.4025
0.0812 0.5757 0.5731
0.1068 0.5774 0.4025
0.0404 0.3687 0.3687
0.034 0.4806 0.6148
0.0404 0.4156 0.615
0.22 0.5653 0.5638
0.0768 0.5041 0.5757
0.3909 0.5781 0.4025
0.0532 0.5647 0.5602
0.22 0.5653 0.5638
0.0532 0.7396 0.7347
0.3359 0.7402 0.6821
0.2517 0.6373 0.6352
0.4113 0.5781 0.4025
0.4641 0.7131 0.713
0.0768 0.5041 0.5757
0.3743 0.5769 0.4025
0.4033 0.5781 0.4025
0.0305 0.4025 0.4025
0.3403 0.5765 0.4025
0.0188 0.4025 0.4025
0.0327 0.3853 1
0.325 0.5786 0.4025
0.2336 0.5178 0.3397
0.1897 0.3508 0.5706
0.1544 0.5778 0.4025
0.0532 0.7396 0.7347
0.3862 0.5778 0.4025
0.2636 0.3504 0.3504
0.3883 0.5784 0.4025
0.1494 0.4363 0.2365
0.0532 0.7196 0.8184
0.0468 0.5618 0.8242
0.0349 0.4603 0.5657
0.325 0.5786 0.4025
0.0404 1 1
0.3909 0.5781 0.4025
0.3909 0.5781 0.4025
0.0335 0.4354 1
0.3555 0.5779 0.4025
0.0404 0.3687 0.3687
0.369 0.5779 0.4025
0.3762 0.5777 0.4025
0.1018 0.9553 0.9465
0.0404 0.5641 1
0.3873 0.5785 0.5779
0.2517 0.6373 0.6352
0.329 0.5743 0.4025
0.3916 0.5783 0.5755
0.4077 0.578 0.5779
0.3874 0.578 0.5595
0.2675 0.5774 0.4025
0.369 0.5779 0.4025
0.2385 0.4727 0.3407
0.1897 0.3508 0.5706
0.2385 0.4727 0.3407
0.3909 0.5781 0.4025
0.0462 0.5644 0.4025
0.3558 0.5782 0.5782
0.0349 0.4552 0.5657
0.3854 0.5779 0.4025
0.3916 0.5783 0.5755
0.3535 0.5779 0.4025
0.0532 0.5551 0.6808
0.2517 0.6373 0.6352
0.2385 0.4727 0.3407
0.0404 0.4878 0.6169
0.4077 0.578 0.5779
0.1897 0.3508 0.5706
0.3766 0.578 0.4025
0.246 0.8583 0.8534
0.1037 0.9668 0.9601
0.3909 0.5781 0.4025
0.2481 0.5769 0.4025
0.3509 0.5779 0.4025
0.0188 0.5333 0.4025
0.2373 0.5055 0.5053
0.3909 0.5781 0.4025
0.0434 0.8149 0.7347
0.22 0.5653 0.5638
0.2517 0.6373 0.6352
0.3767 0.5768 0.4025
0.0404 0.4156 0.615
0.1897 0.3508 0.5706
0.2083 0.4539 0.2558
0.2517 0.6373 0.6352
0.2517 0.6373 0.6352
0.207 0.3516 0.8746
0.3271 0.5743 0.4025
0.0188 0.3221 0.5502
0.1726 0.7983 0.7666
0.0404 0.5227 1
0.2373 0.5055 0.5053
0.0643 0.327 0.2049
0.0349 0.3631 0
0.1256 0.5782 0.5432
0.0532 0.3909 0.5602
0.033 0.385 1
0.1081 0.4542 0.3141
0.3815 0.5785 0.5785
0.3928 0.5781 0.4025
0.0349 0.4552 0.5657
0.3767 0.5768 0.4025
0.2517 0.6373 0.6352
0.3869 0.5778 0.4025
0.3514 0.5777 0.4025
0.2517 0.6373 0.6352
0.355 0.5778 0.4025
0.0349 0.4552 0.5657
0.3214 0.5333 0.4025
0.2332 0.5217 0.4133
0.2083 0.4539 0.2558
0.3214 0.5333 0.4025
0.3869 0.5778 0.4025
0.22 0.5653 0.5638
0.1397 0.5769 0.4025
0.3743 0.5769 0.4025
0.2543 0.3498 0.3498
0.2517 0.6373 0.6352
0.3762 0.5777 0.4025
0.22 0.5653 0.5638
0.2517 0.6373 0.6352
0.2517 0.6373 0.6352
0.1757 0.8016 0.7686
0.2336 0.5178 0.3397
0.0349 0.4552 0.5657
0.3944 0.5773 0.4025
0.1054 0.818 0.7132
0.1645 0.4173 0.3325
0.3767 0.5768 0.4025
0.0349 0.4552 0.5657
0.1384 0.8301 0.7961
0.1862 0.5783 0.5767
0.2517 0.6373 0.6352
0.22 0.5653 0.5638

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TP0341 (Treponema pallidum), UDP-N-acetylmuramate--L-alanine ligase
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