Detailed view for Rv0957

Basic information

TDR Targets ID: 6961
Mycobacterium tuberculosis, Probable bifunctional purine biosynthesis protein PurH: phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transfo
Source Database / ID:  Tuberculist 

pI: 5.6742 | Length (AA): 523 | MW (Da): 55026 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01808   AICARFT/IMPCHase bienzyme
PF02142   MGS-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0003937   IMP cyclohydrolase activity  
GO:0003824   catalytic activity  
GO:0004643   phosphoribosylaminoimidazolecarboxamide formyltransferase activity  
GO:0006188   IMP biosynthetic process  
GO:0006164   purine nucleotide biosynthetic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 523 3zzm (A) 4 523 99.99 0 1 2.13886 -0.53
10 135 1wo8 (A) 1 125 20.00 0 0.93 0.445218 -0.72

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3ZZM:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4A1O:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_127961)

Species Accession Gene Product
Arabidopsis thaliana AT2G35040   AICARFT/IMPCHase bienzyme family protein
Candida albicans CaO19.492   Bifunctional transforymlase/cyclohydrolase
Candida albicans CaO19.8122   Bifunctional transforymlase/cyclohydrolase
Dictyostelium discoideum DDB_G0277087   AICAR transformylase / IMP cyclohydrolase
Drosophila melanogaster Dmel_CG11089   CG11089 gene product from transcript CG11089-RA
Escherichia coli b4006   fused IMP cyclohydrolase/phosphoribosylaminoimidazolecarboxamide formyltransferase
Homo sapiens ENSG00000138363   5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Mycobacterium leprae ML0161   Probable bifunctional purine biosynthesis protein PurH : phosphoribosylaminoimidazolecarboxamide formyltransferase (aicar transf
Mus musculus ENSMUSG00000026192   5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Mycobacterium tuberculosis Rv0957   Probable bifunctional purine biosynthesis protein PurH: phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transfo
Mycobacterium ulcerans MUL_4712   bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
Oryza sativa 4344931   Os08g0206600
Saccharomyces cerevisiae YLR028C   bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase ADE16
Saccharomyces cerevisiae YMR120C   bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase ADE17
Schmidtea mediterranea mk4.001379.09   Bifunctional purine biosynthesis protein PURH
Schmidtea mediterranea mk4.001379.10   Bifunctional purine biosynthesis protein PURH
Wolbachia endosymbiont of Brugia malayi Wbm0411   AICAR transformylase/IMP cyclohydrolase PurH

Essentiality

Rv0957 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu972 this record Mycobacterium tuberculosis essential nmpdr
b4006 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mus musculus 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase Compounds References
Homo sapiens 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0559 0.3754 1
0.0474 1 1
0.0527 0.3018 0.8742
0.0105 0.5211 0.7039
0.0245 1 0.5
0.0293 1 1
0.0238 1 1
0.0238 0.8166 1
0.0261 1 1
0.0533 0.4513 1
0.0242 0.4521 1
0.0234 0.771 1
0.047 0.7498 1
0.0293 0.8473 1
0.0251 0.3167 1
0.0236 0.339 1
0.0544 0.4021 1
0.0247 0.3514 1
0.0241 1 1
0.0523 0.4792 1
0.0281 0.2816 1
0.0293 0.8211 1
0.0238 0.2748 1
0.0236 0.4715 1
0.0254 0.5226 1
0.0543 0.3236 1
0.0237 0.3862 0.8124
0.0235 1 1
0.0293 1 1
0.0559 0.3754 1
0.0282 0.316 0
0.0574 0.4126 1
0.0535 0.2535 0.5181
0.0476 0.7876 1
0.0293 0.8473 1
0.0235 1 1
0.0586 0.2686 1
0.0238 0.2845 1
0.0293 1 1
0.0241 1 1
0.0247 0.3514 1
0.0586 0.2686 1
0.0253 0.5072 0.5
0.0537 0.4456 1
0.0244 1 1
0.0495 1 1
0.0518 1 1
0.0236 0.4677 1
0.0272 1 1
0.0533 0.4513 1
0.0257 0.5108 1
0.0478 0.5602 1
0.0523 0.4792 1
0.0527 0.3018 0.8742
0.0293 1 1
0.0235 0.5257 0.8388
0.0293 0.3207 0.5
0.0293 0.5 0.5
0.0535 0.2535 0.5181
0.0284 0.2937 0.3939
0.0241 0.4898 0.9988
0.0293 1 1
0.052 0.4071 1
0.025 0.5109 0.5
0.0247 0.4052 0.7629
0.0284 0.2937 0.3939
0.0241 0.725 1
0.0242 0.2639 0.5529
0.0244 0.7331 1
0.0196 1 0.5
0.0257 0.3272 1
0.0284 0.2937 0.3939

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier Rv0957 (Mycobacterium tuberculosis), Probable bifunctional purine biosynthesis protein PurH: phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transfo
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