Detailed view for Tb927.4.3420

Basic information

TDR Targets ID: 16471
Trypanosoma brucei, wee1-like protein kinase
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.5569 | Length (AA): 603 | MW (Da): 66218 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
88 530 3pfq (A) 169 629 22.00 0 1 0.73236 1.1
181 603 1koa (A) 5923 6285 24.00 0 1 0.717193 1.02
199 348 3ot8 (A) 8 150 41.00 0.000000015 0.93 0.520956 0.19
200 566 5dvr (A) 51 357 29.00 0.0000024 0.99 0.597824 0.68
200 541 2acx (A) 186 507 20.00 0 1 0.596864 0.28
200 350 3uc3 (A) 22 165 30.00 0.000033 1 0.590615 -0.32
222 365 2pmi (A) 29 167 30.00 0.044 1 0.544006 0.13

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129875)

Species Accession Gene Product
Arabidopsis thaliana AT1G02970   Wee1-like protein kinase
Dictyostelium discoideum DDB_G0291133   WEE1 family protein kinase
Dictyostelium discoideum DDB_G0277539   WEE1 family protein kinase
Drosophila melanogaster Dmel_CG4488   Wee1 ortholog (S. pombe)
Homo sapiens ENSG00000166483   WEE1 G2 checkpoint kinase
Leishmania braziliensis LbrM.20.0940   serine/threonine-protein kinase, putative,protein kinase, putative
Leishmania braziliensis LbrM.29.2780   protein kinase, putative,serine/threonine protein kinase, putative
Leishmania donovani LdBPK_292830.1   protein kinase, putative
Leishmania donovani LdBPK_340990.1   Wee1-like protein kinase, putative
Leishmania infantum LinJ.34.0990   serine/threonine-protein kinase, putative,protein kinase, putative
Leishmania infantum LinJ.29.2830   protein kinase, putative,serine/threonine protein kinase, putative
Leishmania major LmjF.34.0940   serine/threonine-protein kinase, putative,protein kinase, putative
Leishmania major LmjF.29.2720   protein kinase, putative,serine/threonine protein kinase, putative
Leishmania mexicana LmxM.33.0940   serine/threonine-protein kinase, putative,protein kinase, putative
Leishmania mexicana LmxM.08_29.2720   protein kinase, putative,serine/threonine protein kinase, putative
Mus musculus 22390   WEE 1 homolog 1 (S. pombe)
Oryza sativa 4328224   Os02g0135300
Schmidtea mediterranea mk4.000246.12   Wee1-like protein kinase
Schmidtea mediterranea mk4.000053.15   Wee1-like protein kinase
Trypanosoma brucei gambiense Tbg972.4.3430   serine/threonine-protein kinase, putative,protein kinase, putative
Trypanosoma brucei Tb927.4.3420   wee1-like protein kinase
Trypanosoma congolense TcIL3000_4_3120   Wee1-like protein kinase, putative
Trypanosoma cruzi TcCLB.508153.570   Wee1-like protein kinase, putative
Trypanosoma cruzi TcCLB.507485.90   Wee1-like protein kinase, putative
Trypanosoma cruzi TcCLB.506695.30   Wee1-like protein kinase, putative

Essentiality

Tb927.4.3420 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.3420 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.3420 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.3420 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.3420 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens WEE1 G2 checkpoint kinase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0178 0.2956 0.7772
0.0188 0.3534 0.5
0.0032 0.5 0.5
0.0004 0.5 0.5
0.0299 0.323 1
0.0908 0.3773 1
0.0126 0.3029 1
0.1719 0.3661 1
0.461 0.4722 1
0.1212 0.2936 1
0.1323 0.3588 1
0.4748 0.335 1
0.3536 0.3287 1
0.0069 1 1
0.0069 0.3127 0.5
0.043 0.4052 0.9075
0.0176 0.4692 1
0.0372 1 0.5
0.2089 0.3694 1
0.3733 0.4553 1
0.2673 0.3639 1
0.0069 0.3007 0.5
0.0069 0.3593 0.2904
0.4061 0.3788 1
0.3276 0.3459 1
0.0026 0.5 0.5
0.0171 0.3305 1
0.279 0.3735 1
0.3294 0.375 1
0.3364 0.3727 1
0.0032 0.5 0.5
0.0069 0.2794 0.5
0.1083 0.3876 1
0.0116 0.2934 1
0.0057 1 1
0.0624 0.4082 1
0.0042 0.5 0.5
0.2406 0.3568 1
0.4071 0.2783 1
0.023 0.4557 1
0.3811 0.3305 1
0.0007 0.5 0.5
0.4407 0.351 1
0.045 0.7798 1
0.1874 0.3506 1
0.129 0.3236 1
0.518749 0.31468 1
0.0069 1 0.5
0.3962 0.3302 1
0.3845 0.335 1
0.0062 0.6935 0
0.2567 0.311 1
0.3209 0.2783 1
0.0163 0.3088 0.4956
0.0011 1 0.5
0.1728 0.3947 1
0.0018 0.5 0.5
0.0547 0.3229 1
0.0344 0.374 1
0.60287 0.339111 1
0.0014 0.3543 0.399
0.0434 0.5068 1
0.106 0.32 1
0.0016 0.5 0.5
0.0178 0.9754 1
0.0069 0.3352 1
0.0061 0.6883 0.5304
0.414 0.3414 1
0.8686 0.3724 1
0.0056 0.301 0.5
0.0162205 0.425838 1
0.4345 0.3362 1
0.2266 0.3149 1
0.2025 0.3124 1
0.0509 0.8674 0.8668
0.0301 1 1
0.147 0.3512 1
0.2702 0.3577 1
0.0492 0.4355 1
0.0059 1 1
0.2156 0.3686 1
0.1066 0.3797 1
0.3294 0.375 1
0.0875 0.4341 1
0.292 0.3953 1
0.3375 0.3248 1
0.2993 0.3288 1
0.4061 0.3788 1
0.0461 0.2765 0.2762
0.0178 0.2956 0.7772
0.012 0.58 1
0.0759 0.3873 1
0.1443 0.3378 1
0.0547 0.3229 1
0.0063 0.7244 0.2543
0.1551 0.3261 1
0.0007 0.5 0.5
0.1874 0.3506 1
0.1723 0.3629 1
0.0039 0.5 0.5
0.0959 0.3184 1
0.5135 0.4366 1
0.1199 0.4224 1
0.0761 0.3136 1
0.0178 0.9754 1
0.0012 0.5 0.5
0.0758 0.4002 1
0.1407 0.354 1
0.0477 0.5544 1
0.1847 0.3281 1
0.1551 0.3261 1
0.4897 0.3393 1
0.0069 0.5 0.5
0.0064 0.3377 0
0.0832 0.336 1
0.461 0.4722 1
0.0029 0.5 0.5
0.0526 0.3062 1
0.2393 0.348 1
0.2565 0.3471 1
0.8686 0.3724 1
0.1594 0.3651 1
0.0822 0.3143 1
0.3341 0.3278 1
0.3061 0.368 1
0.0114 0.4795 1
0.0438 1 1
0.2268 0.3078 1
0.0699 0.4811 1
0.1175 0.3368 1
0.3505 0.3279 1
0.4723 0.518 1
0.0959 0.3184 1
0.4543 0.3348 1
0.3206 0.2685 1
0.0183 0.3329 1
0.0509 0.8674 0.8668
0.1538 0.4444 1
0.3141 0.3223 1
0.1326 0.4767 1
0.0178 1 0.5
0.3416 0.3403 1
0.2536 0.3584 1
0.0069 0.3296 1
0.0954 0.3371 1
0.3007 0.2535 0.5334
0.3215 0.3222 1
0.018 0.3092 1
0.2831 0.3202 1
0.2495 0.3107 1
0.1065 0.3357 1
0.414 0.341 1
0.1146 0.3032 1
0.0127 0.3015 1
0.0039 0.5 0.5
0.0127 0.3015 1
0.2293 0.3577 1
0.3842 0.3394 1
0.2041 0.3301 1
0.3505 0.3279 1
0.0239973 1 1
0.0127 1 1
0.137211 0.33992 0.605481
0.1992 0.3967 1
0.40724 0.359078 1
0.1146 0.3032 1
0.0056 0.4093 1
0.1719 0.3661 1
0.0022 0.5 0.5
0.0288 0.536 1
0.0572 0.4963 1
0.1407 0.354 1
0.0069 1 1
0.0647 0.4104 1
0.0061 0.6048 0.7627
0.2536 0.3584 1
0.0194 1 1
0.1066 0.3797 1
0.04 0.3298 1
0.0061 0.8224 0.5
0.0822 0.3132 1
0.0175 1 0.5
0.0023 0.5 0.5
0.8686 0.3724 1
0.357682 0.360823 1
0.0125 0.2785 0.5
0.2007 0.3204 1
0.0081 0.5 0.5
0.264 0.3549 1
0.1211 0.3082 1
0.3294 0.3222 1
0.4688 0.4265 1
0.2495 0.3107 1
0.0171 0.3113 1
0.2537 0.3568 1
0.0098 0.3242 0.2614
0.4502 0.3453 1
0.1394 0.3248 1
0.0122 1 1
0.0294 1 1
0.0081 1 0.5
0.0461 0.2765 0.2762
0.1874 0.3506 1
0.0968 0.3929 1
0.0568 0.3815 1
0.0093 0.8828 0
0.3607 0.4412 1
0.2098 0.348 1
0.0184 0.7917 1
0.0644 1 1
0.0163 0.3353 0.5
0.1199 0.4283 1
0.0433 0.7091 1
0.0404 0.3279 1
0.0069 0.3091 1
0.0039 0.9485 0.5
0.0239 1 1
0.0091 1 0.5
0.40724 0.359078 1
0.414 0.3414 1
0.0759 0.3873 1
0.0875 0.4341 1
0.0188 0.3534 0.5
0.279 0.3735 1
0.0423 1 1
0.0239 1 0.5
0.0434 0.5068 1
0.278 0.3271 1
0.3583 0.3287 1
0.0012 0.5 0.5
0.0069 0.317 1
0.2 0.3967 1
0.4748 0.335 1
0.0244 0.293 0.5
0.0501 1 0.5
0.0069 0.2867 1
0.0595 0.3363 1
0.3299 0.359 1
0.0092 1 0.5
0.0127 0.3015 1
0.1551 0.3261 1
0.0069 0.2849 1
0.2756 0.3191 1
0.0595 0.3363 1
0.0975 0.3371 1
0.0179 1 1
0.0514 0.3026 1
0.3583 0.3287 1
0.2271 0.3075 1
0.0069 0.3067 1
0.4562 0.3572 1
0.0027 1 0.5
0.3227 0.2554 0.5883
0.0067 0.5 0.5
0.0866 0.3605 1
0.0088 0.4477 0.5
0.3623 0.3701 1
0.0012 0.5 0.5
0.3733 0.4553 1
0.0392 0.3378 1
0.0069 1 0.5
0.0016 0.5 0.5
0.077 0.5488 1
0.0432 1 1
0.0069 1 0.5
0.0008 0.5 0.5
0.0037 1 0.5
0.1398 0.3232 1
0.0003 0.5 0.5
0.3947 0.3684 1
0.278 0.3271 1
0.0059 1 1
0.117 0.4075 1
0.0829 0.4682 1
0.1469 0.3251 1
0.0115 1 1
0.8686 0.3724 1
0.0056 1 0.5
0.3957 0.3579 1
0.0397 0.3386 1
0.8686 0.3724 1
0.1281 0.4287 1
0.0304 0.5611 1
0.0069 0.5 0.5
0.0033 1 0.5
0.2689 0.4007 1
0.0187 1 1
0.0375 0.9118 0.5
0.3423 0.2613 0.58
0.2993 0.3288 1
0.2847 0.3212 1
0.127902 0.310761 1
0.2293 0.3575 1
0.1393 0.3518 1
0.1814 0.3092 1
0.0445 0.7806 1
0.0829 0.3166 1
0.1874 0.3506 1
0.2457 0.3959 1
0.357682 0.360823 1
0.1719 0.3661 1
0.0789 0.4442 1
0.0069 0.2568 1
0.0057 0.3001 0.5
0.0479 0.3349 1
0.0959 0.3184 1
0.049 0.4489 1
0.013 0.3154 1
0.3206 0.2685 1
0.0115 0.4531 1
0.0059 1 1
0.0007 0.5 0.5
0.2268 0.3075 1
0.0036 0.5 0.5
0.0643 0.4071 1
0.4066 0.3266 1
0.2495 0.3107 1
0.18 0.376 1
0.6413 0.3435 1
0.0568 0.3815 1
0.0406 0.4537 1
0.4345 0.3362 1
0.0033 0.5 0.5
0.4968 0.5027 1
0.0114 0.6188 0.5
0.1874 0.3506 1
0.1097 1 1
0.5135 0.4366 1
0.3872 0.3738 1
0.264 0.3549 1
0.3215 0.3222 1
0.402987 0.305525 1
0.0057 1 1
0.0633 1 1
0.3341 0.3278 1
0.2447 0.375 1
0.0069 0.4034 1
0.0066 0.3101 0
0.0063 1 0.5
0.0433 0.7091 1
0.0182 0.9311 0.5
0.083 0.3611 1
0.1535 0.3672 1
0.075 0.3352 1

Assayability

Assay information

  • Assay for Protein Kinase C (2.7.1.37 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

150 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.4.3420 (Trypanosoma brucei), wee1-like protein kinase
Title for this comment
Comment