Detailed view for Tb927.5.1250

Basic information

TDR Targets ID: 10595
Trypanosoma brucei, GAF domain/TIP41-like family, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.5907 | Length (AA): 576 | MW (Da): 63921 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04176   TIP41-like family
PF13185   GAF domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130873)

Species Accession Gene Product
Candida albicans CaO19.12999   similar to S. cerevisiae YKL069W
Candida albicans CaO19.5553   similar to S. cerevisiae YKL069W
Dictyostelium discoideum DDB_G0271136   GAF domain-containing protein
Escherichia coli b1832   free methionine-(R)-sulfoxide reductase
Leishmania braziliensis LbrM.23.1700   hypothetical protein, conserved
Leishmania donovani LdBPK_231860.1   GAF domain/TIP41-like family, putative
Leishmania infantum LinJ.23.1860   hypothetical protein, conserved
Leishmania major LmjF.23.1460   hypothetical protein, conserved
Leishmania mexicana LmxM.23.1460   hypothetical protein, conserved
Saccharomyces cerevisiae YKL069W   hypothetical protein
Trypanosoma brucei gambiense Tbg972.5.1730   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.1250   GAF domain/TIP41-like family, putative
Trypanosoma congolense TcIL3000_5_1230   GAF domain/TIP41-like family, putative
Trypanosoma cruzi TcCLB.509601.120   GAF domain/TIP41-like family, putative
Trypanosoma cruzi TcCLB.509767.120   GAF domain/TIP41-like family, putative
Trichomonas vaginalis TVAG_190440   conserved hypothetical protein

Essentiality

Tb927.5.1250 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.1250 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.1250 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.1250 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.5.1250 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b1832 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.2789 0.3321 1
0.2545 0.2996 1
0.0826 0.3555 1
0.2503 0.3013 1
0.2584 0.3484 1
0.1123 0.3528 1
0.2307 0.2981 1
0.2574 0.3427 1
0.2937 0.3014 1
0.2514 0.2991 1
0.2416 0.299 1
0.0849 0.2566 1
0.1429 0.3543 1
0.1072 0.3535 1
0.1293 0.3533 1
0.1293 0.3539 1
0.1437 0.2839 1
0.1175 0.3154 1
0.2089 0.3472 1
0.1107 0.3562 1
0.0785 0.2566 1
0.1947 0.34 1
0.119 0.3493 1
0.1743 0.3024 1
0.2725 0.3504 1
0.2789 0.3321 1
0.2596 0.2993 1
0.0607 0.3312 1
0.2396 0.3482 1
0.099 0.3226 1
0.1898 0.2938 1
0.2468 0.2984 1
0.165 0.2894 1
0.2227 0.2965 1
0.2698 0.3006 1
0.2256 0.2948 1
0.0826 0.3555 1
0.0046 0.2566 1
0.1385 0.2566 1
0.0320809 0.508055 1
0.1546 0.3218 1
0.2789 0.3321 1
0.2249 0.3486 1
0.2082 0.2944 1
0.1388 0.2566 1
0.2789 0.3321 1
0.0629 0.3167 1
0.127 0.2566 1
0.2978 0.3014 1
0.2071 0.3498 1
0.2789 0.3321 1
0.2437 0.296 1
0.0902 0.3512 1
0.0968 0.3511 1
0.1293 0.3533 1
0.2435 0.2988 1
0.2503 0.3013 1
0.2733 0.3005 1
0.1107 0.3562 1
0.1078 0.2723 1
0.1417 0.2566 1
0.2018 0.2953 1
0.1391 0.3542 1
0.1615 0.3563 1
0.211 0.3494 1
0.0151 0.2615 1
0.211 0.3494 1
0.2789 0.3321 1
0.1227 0.2566 1
0.2018 0.2953 1
0.2573 0.2983 1
0.2392 0.2982 1
0.1903 0.3022 1
0.1979 0.3497 1
0.27 0.2983 1
0.1617 0.3564 1
0.1403 0.3573 1
0.2979 0.3015 1
0.1879 0.2905 1
0.2563 0.2566 1
0.1221 0.3559 1
0.0849 0.2566 1
0.1293 0.3533 1
0.1256 0.2566 1
0.0235 0.3315 1
0.1896 0.2913 1
0.255 0.2995 1
0.2022 0.3349 1
0.1615 0.3563 1
0.1892 0.293 1
0.1123 0.3528 1
0.1591 0.3574 1
0.0413 0.3161 1
0.2178 0.2964 1
0.0785 0.2566 1
0.2574 0.3427 1
0.2614 0.3191 1
0.2773 0.3007 1
0.1615 0.3563 1
0.2574 0.3427 1
0.149 0.3568 1
0.1293 0.3533 1
0.1615 0.3563 1
0.0129 0.2774 1
0.1807 0.2909 1
0.1615 0.3563 1
0.1338 0.2566 1
0.2614 0.3191 1
0.1221 0.3559 1
0.2541 0.2994 1
0.1342 0.3563 1
0.1615 0.3563 1
0.2392 0.2987 1
0.1507 0.286 1
0.0209 0.3006 1
0.0383 0.2566 1
0.2601 0.2997 1
0.1849 0.2909 1
0.2832 0.3014 1
0.1623 0.3007 1
0.211 0.3494 1
0.1429 0.3543 1
0.2825 0.3009 1
0.1039 0.2566 1
0.251 0.2975 1
0.2563 0.2566 1
0.2243 0.2974 1
0.2532 0.2981 1
0.2921 0.3018 1
0.0479 0.2566 1
0.1447 0.2566 1
0.1293 0.3533 1
0.2367 0.2985 1
0.1401 0.3556 1
0.2089 0.3472 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.5.1250 (Trypanosoma brucei), GAF domain/TIP41-like family, putative
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