Detailed view for TGME49_239620

Basic information

TDR Targets ID: 261284
Toxoplasma gondii, 5'-nucleotidase, C-terminal domain-containing protein
Source Database / ID:  ToxoDB 

pI: 6.5378 | Length (AA): 884 | MW (Da): 95852 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00149   Calcineurin-like phosphoesterase
PF02872   5'-nucleotidase, C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016787   hydrolase activity  
GO:0009166   nucleotide catabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
88 819 1hp1 (A) 27 534 19.00 0 1 0.23 1.14
90 332 1hp1 (A) 29 255 27.00 0.000031 1 0.34 -0.54
95 828 3ztv (A) 37 582 29.00 0.000000000036 1 0.661217 0.99
583 833 2z1a (A) 304 530 35.00 0 1 0.617137 -0.45

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127246)

Species Accession Gene Product
Cryptosporidium hominis Chro.50026   RIKEN cDNA 4933425L06
Cryptosporidium parvum cgd5_3460   nucleotidase (5-nucleotidase/2-cyclic phosphodiesterase) of the calcineurin superfamily
Drosophila melanogaster Dmel_CG30103   CG30103 gene product from transcript CG30103-RA
Drosophila melanogaster Dmel_CG42249   CG42249 gene product from transcript CG42249-RC
Drosophila melanogaster Dmel_CG11883   CG11883 gene product from transcript CG11883-RD
Drosophila melanogaster Dmel_CG30104   Ecto-5'-nucleotidase 2
Drosophila melanogaster Dmel_CG4827   CG4827 gene product from transcript CG4827-RB
Escherichia coli b0480   bifunctional UDP-sugar hydrolase/5'-nucleotidase
Homo sapiens ENSG00000135318   5'-nucleotidase, ecto (CD73)
Mus musculus ENSMUSG00000021718   RIKEN cDNA 4933425L06 gene
Mus musculus ENSMUSG00000032420   5' nucleotidase, ecto
Neospora caninum NCLIV_016240   5-Nucleotidase domain protein (Precursor), related
Schistosoma japonicum Sjp_0301080   ko:K01081 5'-nucleotidase [EC3.1.3.5], putative
Schistosoma mansoni Smp_194100   23-cyclic-nucleotide 2-phosphodiesterase
Schistosoma mansoni Smp_136060   23-cyclic-nucleotide 2-phosphodiesterase
Schmidtea mediterranea mk4.040187.01   Putative 2,3-cyclic-nucleotide 2-phosphodiesterase
Schmidtea mediterranea mk4.007833.04   Putative 2,3-cyclic-nucleotide 2-phosphodiesterase
Schmidtea mediterranea mk4.045113.00  
Toxoplasma gondii TGME49_239620   5'-nucleotidase, C-terminal domain-containing protein
Treponema pallidum TP0104   5'-nucleotidase (ushA)

Essentiality

TGME49_239620 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
b0480 Escherichia coli non-essential goodall
TGME49_239620 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus 5'-nucleotidase Compounds References
Homo sapiens 5'-nucleotidase, ecto (CD73) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0985 0.7448 1
0.3645 0.5669 1
0.0541 0.2812 1
0.3958 0.5147 1
0.3663 0.625 1
0.0146 0.2574 1
0.0143 0.7728 1
0.1849 0.2536 0.3417
0.0141 0.7476 1
0.0449 0.2595 0.3666
0.208 0.4723 1
0.3663 0.625 1
0.169 0.2731 1
0.208 0.4723 1
0.208 0.4723 1
0.0152 0.2606 0.5
0.3663 0.625 1
0.3353 0.5846 1
0.0167 0.3322 1
0.3663 0.625 1
0.3663 0.625 1
0.3513 0.5987 1
0.0272 0.295 1
0.0138 1 1
0.0134 0.3482 1
0.208 0.4723 1
0.2797 0.6338 1
0.0449 0.2595 0.3666
0.1245 0.2585 1
0.3048 0.4895 1
0.2303 0.5191 1
0.1874 0.2572 1
0.0139 0.3495 1
0.3663 0.625 1
0.0143 0.4146 1
0.0287 0.2525 1
0.1849 0.2536 0.3417
0.3663 0.625 1
0.0143 0.4685 1
0.1708 0.5065 1
0.1119 0.2529 1
0.3663 0.625 1
0.3385 0.6221 1
0.3172 0.6597 1
0.076 0.2503 1
0.3786 0.6715 1
0.3172 0.6597 1
0.1123 0.2737 1
0.3335 0.5548 1
0.3663 0.625 1
0.0335 0.7728 1
0.0335 1 1
0.0968 0.265 1
0.0162 0.3764 1
0.1903 0.2569 1
0.0294 0.3538 0.8616
0.208 0.4723 1
0.0136 0.323 1
0.0311 0.4362 1
0.3663 0.625 1
0.3663 0.625 1
0.014 0.577 1
0.3172 0.6597 1
0.0985 0.7448 1
0.3663 0.625 1
0.1891 0.2596 1
0.141 0.2549 1
0.0143 1 1
0.1849 0.2536 0.3417
0.2279 0.4583 1
0.0167 0.4483 1
0.0284 0.3379 1
0.3663 0.625 1
0.014 1 1
0.0167 0.7728 1
0.3663 0.625 1
0.3172 0.6597 1
0.0335 1 1
0.0135 0.3495 1
0.3663 0.625 1
0.0159 0.3302 1
0.0287 0.4566 0.8206
0.1874 0.2568 1
0.3172 0.6597 1
0.0335 1 1
0.208 0.4723 1
0.0168 0.504 1
0.1675 0.2731 1
0.0303 0.6033 1
0.0561 0.3358 1
0.0135 0.3147 1
0.1827 0.2521 1
0.0139 0.3277 1
0.3172 0.6597 1
0.0138 0.4483 1
0.1244 0.3192 1
0.208 0.4723 1
0.0065 1 1
0.3786 0.6715 1
0.208 0.4723 1
0.0168 1 1
0.208 0.4723 1
0.0152 0.2606 0.5
0.3663 0.625 1
0.2368 0.6757 1
0.208 0.4723 1
0.0167 0.7728 1
0.0094 0.3431 0.3406
0.1391 0.289 1
0.0335 1 1
0.3186 0.5149 1
0.3663 0.625 1
0.1025 1 1
0.0556 0.4004 1
0.016 0.6777 1
0.3663 0.625 1
0.1903 0.2569 1
0.0851 0.5897 1
0.0411 0.8075 1
0.208 0.4723 1
0.2141 0.4982 1
0.3172 0.6597 1
0.2453 0.5637 1
0.0335 0.7728 1
0.0167 0.2521 1
0.3958 0.5147 1
0.1891 0.257 1
0.1726 0.6002 1
0.0335 0.7728 1
0.0449 0.2595 0.3666
0.0137 0.2857 1
0.0316 0.6033 1
0.0167 0.7728 1
0.1694 0.2638 1
0.3663 0.625 1
0.0137 0.2513 1
0.168 0.2687 1
0.0134 0.2595 1
0.0143 0.7728 1
0.0136 0.3495 1
0.0088 0.3653 1
0.3663 0.625 1
0.0985 0.7448 1
0.3172 0.6597 1
0.0269 0.6033 1
0.2717 0.6418 1
0.1998 0.382 1
0.0138 0.3495 1
0.0403 0.2862 1
0.0143 0.4146 1
0.0137 0.3593 1
0.0726 1 1
0.0134 0.3495 1
0.1406 0.3112 1
0.0134 0.3646 1
0.3172 0.6597 1
0.1123 0.2737 1
0.2303 0.5191 1
0.2303 0.5191 1
0.1891 0.257 1
0.0751 0.4964 1
0.3663 0.625 1
0.3663 0.625 1
0.0547291 1 1
0.0135 0.3147 1
0.0143 0.5268 1
0.0152 1 1
0.0138 0.2859 1
0.1834 0.251 1
0.208 0.4723 1
0.3663 0.625 1
0.0313 0.3593 0.8777
0.0146 0.4544 1
0.0143 0.6908 1
0.0065 0.2963 0.6941
0.0335 0.6033 1
0.102 0.2525 1
0.014 0.577 1
0.3663 0.625 1
0.0167 0.336 1
0.3663 0.625 1
0.0335 1 1
0.1267 0.2887 1
0.083 0.2993 1
0.0137 0.3495 1
0.0268 0.6145 1
0.0272 0.295 1
0.3663 0.625 1
0.0335 1 1
0.1726 0.6002 1
0.208 0.4723 1
0.0137 0.3522 1
0.1112 0.3286 1
0.0461 0.2705 0.5
0.0166 0.3764 1
0.0335 0.7728 1
0.0318 0.6033 1
0.208 0.4723 1
0.0161 0.3764 1
0.1131 0.4826 1
0.3663 0.625 1
0.0335 0.7728 1
0.2775 0.8539 1
0.3663 0.625 1
0.3172 0.6597 1
0.3663 0.625 1
0.029 0.4553 0.822
0.3663 0.625 1
0.3188 0.7217 1
0.0549 0.6615 1
0.0277 0.2939 0.7266
0.077 0.2572 1
0.0335 0.7728 1
0.2278 0.4519 1
0.3663 0.625 1
0.0142 1 1
0.0167 0.4483 1
0.3172 0.6597 1
0.076 0.2503 1
0.0299 0.4772 0.787
0.0167 0.3764 1
0.3663 0.625 1
0.0167 1 1
0.208 0.4723 1
0.0288 0.4995 0.8607
0.3663 0.625 1
0.1123 0.2737 1
0.0985 0.7448 1
0.0167 0.4679 1
0.0136 0.3482 1
0.0148 0.3764 1
0.3663 0.625 1
0.0167 0.474 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_239620 (Toxoplasma gondii), 5'-nucleotidase, C-terminal domain-containing protein
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