Detailed view for Tb927.10.6950

Basic information

TDR Targets ID: 574372
Trypanosoma brucei, sterol 24-c-methyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.1724 | Length (AA): 359 | MW (Da): 40128 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08241   Methyltransferase domain
PF08498   Sterol methyltransferase C-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0008168   methyltransferase activity  
GO:0006694   steroid biosynthetic process  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129172)

Species Accession Gene Product
Arabidopsis thaliana AT1G76090   24-methylenesterol C-methyltransferase 3
Arabidopsis thaliana AT1G20330   24-methylenesterol C-methyltransferase 2
Arabidopsis thaliana AT5G13710   cycloartenol-c-24-methyltransferase
Brugia malayi Bm1_45250   S-adenosyl-methionine cycloartenol-C24-methyltransferase
Candida albicans CaO19.9199   SAM:delta 24-methyltransferase involved in ergosterol biosynthesis
Candida albicans CaO19.1631   SAM:delta 24-methyltransferase involved in ergosterol biosynthesis
Caenorhabditis elegans CELE_H14E04.1   Protein H14E04.1
Dictyostelium discoideum DDB_G0288907   hypothetical protein
Leishmania braziliensis LbrM.35.2600   sterol 24-c-methyltransferase, putative
Leishmania donovani LdBPK_362510.1   sterol 24-c-methyltransferase, putative
Leishmania infantum LinJ.36.2520   sterol 24-c-methyltransferase, putative
Leishmania infantum LinJ.36.2510   sterol 24-c-methyltransferase, putative
Leishmania major LmjF.36.2380   sterol 24-c-methyltransferase, putative
Leishmania major LmjF.36.2390   sterol 24-c-methyltransferase, putative
Leishmania mexicana LmxM.36.2390   sterol 24-c-methyltransferase, putative
Leishmania mexicana LmxM.36.2380   sterol 24-c-methyltransferase, putative
Loa Loa (eye worm) LOAG_00971   hypothetical protein
Oryza sativa 9269350   Os03g0807600
Oryza sativa 4331540   Os03g0136200
Oryza sativa 4342690   Os07g0206700
Saccharomyces cerevisiae YML008C   sterol 24-C-methyltransferase
Trypanosoma brucei gambiense Tbg972.10.8450   sterol 24-c-methyltransferase, putative
Trypanosoma brucei Tb927.10.6910   Sterol methyltransferase, putative
Trypanosoma brucei Tb11.v5.0496   sterol 24-c-methyltransferase, putative
Trypanosoma brucei Tb927.10.6950   sterol 24-c-methyltransferase, putative
Trypanosoma cruzi TcCLB.505683.10   sterol 24-c-methyltransferase, putative
Trypanosoma cruzi TcCLB.504191.10   sterol 24-c-methyltransferase, putative
Trypanosoma cruzi TcCLB.510185.10   sterol 24-c-methyltransferase, putative

Essentiality

Tb927.10.6950 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.6950 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.6950 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.6950 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.6950 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma cruzi sterol 24-c-methyltransferase, putative Compounds References
Trypanosoma cruzi sterol 24-c-methyltransferase, putative Compounds References
Glycine max S-adenosyl-L-methionine:delta24-sterol-C-methyltransferase Compounds References
Trypanosoma cruzi sterol 24-c-methyltransferase, putative Compounds References
Arabidopsis thaliana cycloartenol-c-24-methyltransferase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Trypanosoma cruzi sterol 24-c-methyltransferase, putative 161 aa 67.1% 161 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0628 1 1
0.0749 0.5913 1
0.0385 0.3017 0.3214
0.0855 1 1
0.0591 1 1
0.0385 0.3017 0.3214
0.0745 0.3576 0.7161
0.0205 1 1
0.0203 0.2865 0.4456
0.0195 1 1
0.0641 1 1
0.0187 1 1
0.0599 0.6503 1
0.0853 1 1
0.0384 0.8815 1
0.00879579 0.706853 1
0.0818 0.4463 1
0.0186 0.4796 1
0.0812 0.6132 1
0.0574 1 1
0.0194 1 1
0.00879579 0.706853 1
0.0196 0.3707 0.3675
0.0377 0.301 0.2708
0.0821 1 1
0.0853 1 1
0.0783 1 1
0.0628 1 1
0.0372 0.2971 0.2971
0.0599 0.6503 1
0.0599 0.6503 1
0.0615 0.3438 1
0.0377 0.2984 0.2708
0.0854 1 1
0.0212 0.2584 0.2603
0.0818 0.4463 1
0.0637 0.6757 1
0.0203 0.6968 1
0.0186 0.3142 1
0.0187 0.4967 0.4965
0.0192 0.4731 1
0.0232 1 1
0.076 1 1
0.0599 0.6503 1
0.0563 1 1
0.0187 1 1
0.0599 0.6503 1
0.0186 0.4459 1
0.0192 0.4728 1
0.0812 0.6132 1
0.0232 1 1
0.0746 1 1
0.0387 0.3057 0.3057
0.019 1 1
0.0591 1 1
0.0188 0.316 1
0.0197 0.7516 0.7489
0.0194 0.3529 0.3304
0.019 0.2679 0.5597
0.0637 0.6757 1
0.0187 0.2924 1
0.0853 1 1
0.0599 0.6503 1
0.0873 1 1
0.0189 1 1
0.0763 0.6137 1
0.0195 0.2681 1
0.0563 1 1
0.0186 0.4459 1
0.0855 1 1
0.0853 1 1
0.0187 0.4624 1
0.0623 0.2985 0.2958
0.0199 0.523 1
0.0194 0.7681 1
0.0818 0.4432 1
0.0853 1 1
0.0415 0.2702 0.2702
0.0203 0.2852 0.6007
0.0192 0.2623 0.9791
0.0583 1 1
0.0193 1 1
0.0599 0.6503 1
0.0812 0.6132 1
0.0199 0.523 1
0.0195 0.2681 1
0.0397 0.4471 1
0.0398 0.3155 0.3155
0.0623 0.2985 0.2958
0.0798 1 1
0.0587 1 1
0.0208 0.4356 1
0.0557 1 1
0.0195 0.331 0.3028
0.0198 0.738 1
0.0208 0.2797 0.4737
0.0206 0.2923 1
0.0187 1 1
0.0398 0.322 0.319
0.0615 0.3438 1
0.0854 1 1
0.078 0.2713 0.7182
0.0615 0.3438 1
0.0603 1 1
0.0208 0.2926 0.3386
0.0374 0.3111 0.3109

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.6950 (Trypanosoma brucei), sterol 24-c-methyltransferase, putative
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