Detailed view for EHI_166850

Basic information

TDR Targets ID: 803753
Entamoeba histolytica, proteasome subunit alpha type 4, putative
Source Database / ID:  AmoebaDB  

pI: 5.4387 | Length (AA): 239 | MW (Da): 26923 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00227   Proteasome subunit
PF10584   Proteasome subunit A N-terminal signature

Gene Ontology

Mouse over links to read term descriptions.
GO:0019773   proteasome core complex, alpha-subunit complex  
GO:0005839   proteasome core complex  
GO:0004298   threonine endopeptidase activity  
GO:0004175   endopeptidase activity  
GO:0051603   proteolysis involved in cellular protein catabolic process  
GO:0006511   ubiquitin-dependent protein catabolic process  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 236 5le5 (B) 11 246 49.00 0 1 1.64815 -0.88
1 237 3h4p (A) 15 244 40.00 0 1 1.42903 0.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127759)

Species Accession Gene Product
Arabidopsis thaliana AT4G15165   N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein
Arabidopsis thaliana AT3G22110   20S proteasome alpha-3 subunit
Babesia bovis BBOV_III009190   proteasome A-type and B-type family protein
Brugia malayi Bm1_17085   proteasome subunit alpha type 4
Candida albicans CaO19.350   proteasome component
Candida albicans CaO19.7983   proteasome component
Caenorhabditis elegans CELE_Y110A7A.14   Protein PAS-3
Cryptosporidium hominis Chro.40039   proteasome subunit
Cryptosporidium parvum cgd4_250   proteasome subunit alpha type 4, NTN hydrolase fold
Dictyostelium discoideum DDB_G0280969   20S proteasome subunit alpha-4
Drosophila melanogaster Dmel_CG9327   Proteasome alpha3 subunit
Echinococcus granulosus EgrG_000864950   proteasome prosome macropain subunit alpha
Entamoeba histolytica EHI_166850   proteasome subunit alpha type 4, putative
Entamoeba histolytica EHI_167720   proteasome subunit alpha type 4, putative
Echinococcus multilocularis EmuJ_000864950   proteasome (prosome macropain) subunit alpha
Giardia lamblia GL50803_14497   20S proteasome alpha subunit 3
Homo sapiens ENSG00000041357   proteasome (prosome, macropain) subunit, alpha type, 4
Leishmania braziliensis LbrM.14.0310   proteasome alpha 3 subunit, putative
Leishmania donovani LdBPK_140310.1   proteasome alpha 3 subunit, putative
Leishmania infantum LinJ.14.0310   proteasome alpha 3 subunit, putative
Leishmania major LmjF.14.0310   proteasome alpha 3 subunit, putative
Leishmania mexicana LmxM.14.0310   proteasome alpha 3 subunit, putative
Loa Loa (eye worm) LOAG_03181   PAS-3 protein
Mus musculus ENSMUSG00000032301   proteasome (prosome, macropain) subunit, alpha type 4
Neospora caninum NCLIV_016120   proteasome subunit alpha type 4, subunit, putative
Oryza sativa 4340285   Os06g0176000
Oryza sativa 4340253   Os06g0167600
Plasmodium berghei PBANKA_1130400   proteasome subunit alpha type-4, putative
Plasmodium falciparum PF3D7_1353800   proteasome subunit alpha type-4, putative
Plasmodium knowlesi PKNH_1117900   proteasome subunit alpha type-4, putative
Plasmodium vivax PVX_114680   proteasome subunit alpha type-4, putative
Plasmodium yoelii PY02352   proteasome subunit alpha type 4
Saccharomyces cerevisiae YGR135W   proteasome core particle subunit alpha 3
Schistosoma japonicum Sjp_0210950   ko:K02728 20S proteasome subunit alpha 3, putative
Schistosoma mansoni Smp_070930   proteasome subunit alpha 4 (T01 family)
Schmidtea mediterranea mk4.034879.00   Proteasome subunit alpha type
Schmidtea mediterranea mk4.005109.00   Proteasome subunit alpha type
Schmidtea mediterranea mk4.019303.00   Proteasome subunit alpha type
Schmidtea mediterranea mk4.000040.09   Proteasome subunit alpha type
Schmidtea mediterranea mk4.001251.08   Proteasome subunit alpha type
Schmidtea mediterranea mk4.002984.01   Proteasome subunit alpha type
Trypanosoma brucei gambiense Tbg972.7.4990   proteasome alpha 3 subunit, putative
Trypanosoma brucei Tb927.7.4420   proteasome alpha 3 subunit, putative
Trypanosoma congolense TcIL3000_7_3640   proteasome alpha 3 subunit, putative
Trypanosoma cruzi TcCLB.506985.30   proteasome alpha 3 subunit, putative
Trypanosoma cruzi TcCLB.504213.120   proteasome alpha 3 subunit, putative
Toxoplasma gondii TGME49_239500   proteasome subunit alpha type, putative
Theileria parva TP04_0827   proteasome subunit alpha type, putative
Trichomonas vaginalis TVAG_058050   Family T1, proteasome alpha subunit, threonine peptidase

Essentiality

EHI_166850 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.4420 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.4420 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.4420 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.4420 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y110A7A.14 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y110A7A.14 Caenorhabditis elegans larval arrest wormbase
CELE_Y110A7A.14 Caenorhabditis elegans slow growth wormbase
CELE_Y110A7A.14 Caenorhabditis elegans sterile wormbase
TGME49_239500 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 4 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.014 0.3336 0.4892
0.014 0.3336 0.4892
0.0253 0.335 0.4892
0.0129 0.3336 0.4892
0.014 0.335 0.4892
0.0125 0.354 0.3693
0.0116 0.2766 0.4892
0.0136 0.3325 0.4879
0.0122 0.3543 0.3672
0.0258 0.347 0.5042
0.0244 0.335 0.4892
0.0113 0.3336 0.4892
0.014 0.3336 0.4892
0.014 0.3336 0.4892
0.0265 0.3334 0.4892
0.0261 0.3338 0.4892
0.014 0.335 0.4892
0.0235 0.335 0.4892

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_166850 (Entamoeba histolytica), proteasome subunit alpha type 4, putative
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