Detailed view for TGME49_243610

Basic information

TDR Targets ID: 258395
Toxoplasma gondii, C-5 cytosine-specific DNA methylase superfamily protein
Source Database / ID:  ToxoDB 

pI: 8.3418 | Length (AA): 883 | MW (Da): 96891 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00145   C-5 cytosine-specific DNA methylase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008168   methyltransferase activity  
GO:0003677   DNA binding  
GO:0006306   DNA methylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
346 674 2c7p (A) 3 319 16.00 0 0.88 0.24 0.71
447 550 2c7p (A) 73 174 30.00 0.000081 0.22 0.37 -0.33
1 307 4h5y (A) 119 432 10.00 0.2 0 0.380578 0.18
48 112 3qh9 (A) 14 78 8.00 0.65 0 0.261513 -1.47
374 552 1g55 (A) 25 173 46.00 0 1 0.325318 0.38
431 553 3qv2 (A) 57 179 29.00 0 0.79 0.480198 -0.37

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_130762)

Species Accession Gene Product
Arabidopsis thaliana AT5G25480   DNA methyltransferase-2
Cryptosporidium hominis Chro.50169   DNA methyltransferase PMT1 - like protein
Cryptosporidium parvum cgd5_2100   DNA methyltransferase PMT1 - like protein
Dictyostelium discoideum DDB_G0288047   DNA -methyltransferase
Drosophila melanogaster Dmel_CG10692   Methyltransferase 2
Echinococcus granulosus EgrG_001185500   DNA methyltransferase 2, putative
Entamoeba histolytica EHI_069140   DNA (cytosine-5)-methyltransferase, putative
Echinococcus multilocularis EmuJ_001185500   DNA methyltransferase 2, putative
Homo sapiens 1787   tRNA aspartic acid methyltransferase 1
Mus musculus 13434   tRNA aspartic acid methyltransferase 1
Neospora caninum NCLIV_018330   DNA methyltransferase, putative
Oryza sativa 9268926   Os01g0612000
Schistosoma japonicum Sjp_0040910   ko:K00558 DNA (cytosine-5-)-methyltransferase [EC2.1.1.37], putative
Schistosoma japonicum Sjp_0207250   ko:K00558 DNA (cytosine-5-)-methyltransferase [EC2.1.1.37], putative
Schistosoma mansoni Smp_145390   DNA (cytosine-5)-methyltransferase
Schmidtea mediterranea mk4.000626.03   DNA methyl-transferase
Schmidtea mediterranea mk4.000399.10   Tud-1
Toxoplasma gondii TGME49_243610   C-5 cytosine-specific DNA methylase superfamily protein

Essentiality

TGME49_243610 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_243610 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0864 0.2949 0.6186
0.0976 0.2979 0.6459
0.0059 0.4497 0
0.0404 0.4405 0.4405
0.0059 0.4497 0
0.0126 0.2647 0.505
0.0511 0.2912 0.6449
0.0956 0.2945 0.6186
0.0866 0.6943 0.7154
0.0864 0.2949 0.6186
0.087 0.294 0.6193
0.0913 0.2936 0.6186
0.0663 0.3541 0.6105
0.0582 0.3156 0.7389
0.0486 0.3386 0.5856
0.048 0.256 0.5614
0.0558 0.3185 0.5396
0.0341 0.3073 0
0.023 0.4243 0.4243
0.0477 0.2556 0.5679
0.0952 0.5727 0.7484
0.087 0.2936 0.6186
0.051 0.369 0.5176
0.0158 0.8655 0
0.0518 0.2772 0.6189
0.0911 0.5333 0.8117
0.0072 0.4497 0
0.0956 0.2945 0.6186
0.0864 0.296 0.6227
0.0072 0.4497 0
0.0937 0.4859 0.7223
0.0913 0.2936 0.6186
0.0547 0.3057 0.565
0.0072 0.4497 0
0.0599 0.2531 0.604
0.0048 0.4497 0
0.00431057 0.5 0.5
0.0058 0.3563 0
0.0625 0.3313 0.6734
0.0663 0.3541 0.6105
0.0547 0.3047 0.5641
0.0281 0.295 0
0.0057 0.4497 0
0.0358 0.2591 1
0.0064 0.2961 0.6252
0.088 0.4804 0.7745
0.0951 0.2977 0.6398
0.0096 0.6636 1
0.0072 0.4497 0
0.0068 0.4497 0
0.0225 0.4785 0.4585
0.0937 0.4859 0.7223
0.006 0.4497 0
0.0913 0.2936 0.6186
0.0839 0.4753 0.8769
0.0585 0.3149 0.7398
0.0118 0.3963 0
0.0599 0.2531 0.604
0.0617 0.3335 0.6854
0.0558 0.3087 0.5422
0.0558 0.3044 0.5592
0.0157 0.8655 0
0.0546 0.3077 0.5649
0.0436 0.5275 0.5255
0.0951 0.4896 0.7529
0.0475 0.3182 0.5541
0.0286 0.5274 0.5012
0.0225 0.4785 0.4585
0.0617 0.3335 0.6854
0.0898 0.4876 0.7234
0.0433 0.2757 0
0.0528 0.2882 0.7481
0.0253 0.2572 0
0.0059 0.4497 0
0.0224 0.4393 0.4393
0.087 0.2936 0.6186
0.006 0.4497 0
0.0062 0.4497 0
0.0206 0.3269 0.4686
0.0663 0.3541 0.6105
0.0908 0.5336 0.8119
0.0048 0.7364 0.7364
0.085 0.459 0.6782
0.0157 0.2524 0.9068
0.0625 0.3621 0.5559
0.0157 0.8656 0
0.0581 0.3331 0.6066
0.0913 0.2936 0.6186
0.0864 0.296 0.6227
0.0305 0.5022 0.5022
0.0483 0.2625 0.6859
0.0434 0.3112 0.5283
0.007 0.4497 0
0.0976 0.2979 0.6459
0.0064 0.2961 0.6252
0.0913 0.2936 0.6186
0.0342 0.4442 0.4442
0.0585 0.3337 0.606
0.029 0.2598 0
0.0619 0.3259 0.6621
0.0627 0.3652 0.5707
0.0976 0.2979 0.6459
0.0151 0.3224 0.4896
0.0479 0.3336 0.5818
0.0557 0.4052 0.6848
0.0061 0.4497 0
0.0465 0.3259 0.6273
0.0705 0.3682 0.6524
0.0157 0.8656 0
0.0447 0.3212 0.8587
0.0483 0.2625 0.6859
0.0059 0.366 0
0.0057 0.4497 0
0.0581 0.3331 0.6066
0.0064 0.4497 0
0.0157 0.3941 0.8918
0.0901 0.4859 0.7223
0.0514 0.283 0.6167
0.028 0.2661 0.5877
0.0942 0.486 0.7223
0.087 0.294 0.6193
0.0663 0.3541 0.6105
0.0752 0.3883 0.6786
0.0058 0.4497 0
0.0499 0.2874 0.6366
0.0091 0.3769 1
0.0901 0.3272 0.7235
0.0529 0.3132 0.7237
0.087 0.2936 0.6186
0.0545 0.3072 0.565
0.0497 0.2864 0.6377
0.0157 0.2524 0.9068
0.054 0.3687 0.5891
0.0061 0.4497 0
0.0059 0.3579 0
0.0158 0.8654 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_243610 (Toxoplasma gondii), C-5 cytosine-specific DNA methylase superfamily protein
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