Detailed view for TGME49_307770

Basic information

TDR Targets ID: 260599
Toxoplasma gondii, fumble protein
Source Database / ID:  ToxoDB 

pI: 8.4799 | Length (AA): 1225 | MW (Da): 131977 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03630   Fumble

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004594   pantothenate kinase activity  
GO:0015937   coenzyme A biosynthetic process  

Metabolic Pathways

Pantothenate and CoA biosynthesis (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
341 526 4ehu (A) 71 262 13.00 0 0.04 0.207637 -0.22
576 902 3mk6 (D) 26 365 26.00 0 0.99 0.470739 0.65
598 884 5e26 (A) 255 541 24.00 0 1 0.425086 0.77
615 886 2i7n (A) 297 568 36.00 0 1 0.389141 1.06
616 901 5e26 (A) 272 564 28.00 0 1 0.470269 0.53
821 940 3ami (A) 40 154 30.00 0.84 0.2 0.165059 0.76

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_126914)

Species Accession Gene Product
Arabidopsis thaliana AT1G60440   Pantothenate kinase 1
Arabidopsis thaliana AT4G32180   pantothenate kinase 2
Babesia bovis BBOV_II001350   hypothetical protein
Brugia malayi Bm1_40300   pantothenate kinase family protein
Candida albicans CaO19.13022   putative pantothenate kinase.
Candida albicans CaO19.5576   putative pantothenate kinase.
Candida albicans CaO19.5577   putative pantothenate kinase.
Candida albicans CaO19.13023   putative pantothenate kinase.
Caenorhabditis elegans CELE_C42D8.3   Protein PNK-4
Caenorhabditis elegans CELE_C10G11.5   Protein PNK-1, isoform B
Cryptosporidium hominis Chro.70552   hypothetical protein
Cryptosporidium parvum cgd7_4950   pantothenate kinase 4, rnaseH fold
Dictyostelium discoideum DDB_G0290401   pantothenate kinase
Drosophila melanogaster Dmel_CG5828   CG5828 gene product from transcript CG5828-RB
Drosophila melanogaster Dmel_CG5725   fumble
Echinococcus granulosus EgrG_000497400   pantothenate kinase 4
Entamoeba histolytica EHI_183060   pantothenate kinase 1, putative
Echinococcus multilocularis EmuJ_000497400   pantothenate kinase 4
Giardia lamblia GL50803_5857   Pantothenate kinase 4
Homo sapiens ENSG00000157881   pantothenate kinase 4
Homo sapiens ENSG00000152782   pantothenate kinase 1
Homo sapiens 79646   pantothenate kinase 3
Homo sapiens ENSG00000125779   pantothenate kinase 2
Leishmania braziliensis LbrM.28.0150   pantothenate kinase subunit, putative
Leishmania donovani LdBPK_280140.1   pantothenate kinase subunit, putative
Leishmania infantum LinJ.28.0140   pantothenate kinase subunit, putative
Leishmania major LmjF.28.0140   pantothenate kinase subunit, putative
Leishmania mexicana LmxM.28.0140   pantothenate kinase subunit, putative
Loa Loa (eye worm) LOAG_06143   pantothenate kinase
Mus musculus ENSMUSG00000018846   pantothenate kinase 3
Mus musculus ENSMUSG00000029056   pantothenate kinase 4
Mus musculus ENSMUSG00000033610   pantothenate kinase 1
Mus musculus ENSMUSG00000037514   pantothenate kinase 2
Neospora caninum NCLIV_060530   hypothetical protein
Oryza sativa 4340439   Os06g0207000
Oryza sativa 4347680   Os09g0533100
Onchocerca volvulus OVOC5238   Fumble homolog
Plasmodium berghei PBANKA_1022600   pantothenate kinase 1, putative
Plasmodium falciparum PF3D7_1420600   pantothenate kinase 1, putative
Plasmodium knowlesi PKNH_1337400   pantothenate kinase 1, putative
Plasmodium vivax PVX_085450   pantothenate kinase, putative
Plasmodium yoelii PY06793   hypothetical protein
Saccharomyces cerevisiae YDR531W   pantothenate kinase
Schistosoma japonicum Sjp_0120430   hypothetical protein
Schistosoma japonicum Sjp_0079760   ko:K09680 type II pantothenate kinase, putative
Schistosoma mansoni Smp_064750.2   pantothenate kinase
Schistosoma mansoni Smp_064750.1   pantothenate kinase
Schmidtea mediterranea mk4.009378.05  
Schmidtea mediterranea mk4.010923.00   Pantothenate kinase 4
Schmidtea mediterranea mk4.002538.01  
Schmidtea mediterranea mk4.003254.01  
Schmidtea mediterranea mk4.005749.01   Pantothenate kinase 4
Schmidtea mediterranea mk4.003254.02   Pantothenate kinase 4
Schmidtea mediterranea mk4.009378.02  
Trypanosoma brucei gambiense Tbg972.11.8330   pantothenate kinase subunit, putative
Trypanosoma brucei Tb927.11.7290   pantothenate kinase subunit, putative
Trypanosoma congolense TcIL3000_0_00870   pantothenate kinase subunit, putative
Trypanosoma cruzi TcCLB.506779.80   pantothenate kinase subunit, putative
Trypanosoma cruzi TcCLB.511153.120   pantothenate kinase subunit, putative
Toxoplasma gondii TGME49_307770   fumble protein
Theileria parva TP04_0450   hypothetical protein
Trichomonas vaginalis TVAG_601540   pantothenate kinase, putative
Trichomonas vaginalis TVAG_328400   pantothenate kinase, putative
Trichomonas vaginalis TVAG_562330   pantothenate kinase, putative
Trichomonas vaginalis TVAG_569390   pantothenate kinase, putative
Trichomonas vaginalis TVAG_142730   conserved hypothetical protein
Trichomonas vaginalis TVAG_364740   pantothenate kinase, putative

Essentiality

TGME49_307770 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.5190 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.5190 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.5190 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.5190 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C10G11.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_C10G11.5 Caenorhabditis elegans larval arrest wormbase
CELE_C10G11.5 Caenorhabditis elegans slow growth wormbase
CELE_C10G11.5 Caenorhabditis elegans sterile wormbase
CELE_C42D8.3 Caenorhabditis elegans slow growth wormbase
YDR531W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1022600 Plasmodium berghei Dispensable plasmo
TGME49_307770 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens pantothenate kinase 3 Compounds References
Homo sapiens pantothenate kinase 2 Compounds References
Homo sapiens pantothenate kinase 1 Compounds References
Plasmodium falciparum pantothenate kinase 1, putative Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0088 1 1
0.0188 0.3734 1
0.1705 0.5971 1
0.1099 0.3174 1
0.0087 0.4887 1
0.1753 0.6148 1
0.0086 0.448 0.5
0.167 1 1
0.0078 1 1
0.0084 1 1
0.1177 0.271 1
0.0153 1 0.5
0.1729 0.802 1
0.1728 0.4026 1
0.009 0.4631 1
0.0079 0.6538 1
0.2021 0.4079 1
0.1703 0.6093 1
0.0077 1 0.5
0.1444 0.6899 1
0.1858 0.3302 1
0.0188 0.4645 1
0.0085 0.4029 1
0.0079 0.6538 1
0.0077 0.385 0.4372
0.0077 0.2698 1
0.2106 0.6022 1
0.0083 0.4902 1
0.116 0.274 1
0.1917 0.9253 1
0.2023 0.5645 1
0.0077 0.3653 0.6784
0.2156 0.5127 1
0.1819 0.8723 1
0.1927 1 1
0.1173 0.3072 1
0.1708 0.8017 1
0.1841 0.4279 1
0.0091 0.3242 1
0.2197 0.617 1
0.1728 1 1
0.1597 1 1
0.0084 1 1
0.0082 0.7261 0.7533
0.0077 0.2698 1
0.0086 1 1
0.2007 0.3591 1
0.1825 0.4156 1
0.1737 0.6182 1
0.008 0.2581 1
0.1723 0.4083 1
0.0085 0.4705 1
0.1835 0.3723 1
0.0076 0.3033 0
0.1818 0.4167 1
0.0188 1 0.5
0.0084 0.292 1
0.1677 0.4705 1
0.1758 0.4191 1
0.0092 0.3061 0.5
0.0076 0.3657 1
0.0456 0.5367 1
0.1667 0.7885 1
0.0188 1 0.5
0.1727 0.829 1
0.2034 0.3597 1
0.1349 1 1
0.1822 0.3997 1
0.092 0.4314 1
0.169 0.8078 1
0.0188 1 0.5
0.1744 0.4082 1
0.1761 0.6417 1
0.1708 0.808 1
0.1845 0.5288 1
0.2132 0.377 1
0.2156 0.5127 1
0.1824 0.5072 1
0.1751 1 1
0.0282 1 1
0.0082 0.4905 1
0.0076 0.3655 1
0.1739 0.4079 1
0.1191 0.3905 1
0.1003 0.3307 1
0.077 0.5503 1
0.1896 0.6859 1
0.0084 1 1
0.1429 1 1
0.0085 0.4029 1
0.1708 0.5981 1
0.023 0.3055 1
0.0091 0.2559 1
0.1262 0.5999 1
0.1912 0.9177 1
0.0084 0.2634 1
0.0077 1 0.5
0.2043 0.5845 1
0.0188 1 0.5
0.008 0.5008 1
0.1106 0.3172 1
0.1733 0.8332 1
0.172 0.6141 1
0.0086 0.3979 1
0.0091 0.2792 1
0.171 0.8012 1
0.0081 0.2859 1
0.0089 0.3169 1
0.1828 0.4362 1
0.0079 0.4485 1
0.0079 0.4459 1
0.0188 1 0.5
0.1262 0.5999 1
0.009 0.4631 1
0.1705 0.8468 1
0.2069 0.4678 1
0.1482 1 1
0.0082 0.2535 1
0.1504 0.3964 1
0.0079 0.2686 1
0.1937 0.4645 1
0.185 0.4425 1
0.2212 0.3884 1
0.1738 1 1
0.1917 0.9253 1
0.0077 0.3847 0.4395
0.0091 0.3054 0.5
0.0075 0.288 1
0.0082 0.257 1
0.1104 0.772 1
0.0082 1 1
0.202 0.4789 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_307770 (Toxoplasma gondii), fumble protein
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