Detailed view for Tb927.5.1360

Basic information

TDR Targets ID: 12337
Trypanosoma brucei, nucleoside 2-deoxyribosyltransferase
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.0011 | Length (AA): 153 | MW (Da): 17298 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05014   Nucleoside 2-deoxyribosyltransferase

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 152 2f62 (A) 9 160 98.00 0 1 2.20926 -1.47

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2A0K:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2F2T:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2F62:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2F64:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2F67:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_135187)

Species Accession Gene Product
Leishmania braziliensis LbrM.23.1820   hypothetical protein, conserved
Leishmania donovani LdBPK_231980.1   Nucleoside 2-deoxyribosyltransferase, putative
Leishmania infantum LinJ.23.1980   hypothetical protein, conserved
Leishmania major LmjF.23.1580   hypothetical protein, conserved
Leishmania mexicana LmxM.23.1580   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.5.1860   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.1360   nucleoside 2-deoxyribosyltransferase
Trypanosoma congolense TcIL3000_0_29990   nucleoside 2-deoxyribosyltransferase
Trypanosoma congolense TcIL3000_0_57260   nucleoside 2-deoxyribosyltransferase
Trypanosoma cruzi TcCLB.504081.20   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506737.100   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510025.230   hypothetical protein
Trypanosoma cruzi TcCLB.508221.539   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508229.50   hypothetical protein

Essentiality

Tb927.5.1360 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.1360 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.1360 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.1360 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.1360 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from bioassay (ECO:0000094) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: Drug: benzo[cd]indol-2(1H)-one, 5-aminoisoquinoline, 1-methyl-2(1H)-quinoline, 2-ethylbenzylalcohol. . References: 17004709
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.054 1 1
0.3077 0.6314 1
0.356 1 1
0.5377 1 1
0.027 0.3801 1
0.3077 0.6314 1
0.2526 0.2952 0.3706
0.2299 0.3555 0.3555
0.0322 0.5 0.5
0.3706 0.768 0.5
0.3077 0.6314 1
0.1813 0.3522 0.3549
0.0322 1 1
0.3077 0.6314 1
0.3077 0.6314 1
0.2526 0.2952 0.3706
0.2332 0.3549 0.3549
0.0262 0.5 0.5
0.0259 0.3199 1
0.0322 0.4669 1
0.0322 0.378 1
0.3077 0.6314 1
0.0322 0.3186 0.5
0.0672 0.4131 1
0.0285 0.325 1
0.3077 0.6314 1
0.3788 1 1
0.0263 0.8315 1
0.0517 0.5 0.5
0.0263 0.2948 1
0.5377 1 1
0.204 0.3551 0.3551
0.1856 0.7894 1
0.3788 1 1
0.2785 0.3707 0.3707
0.026 1 1
0.0258 0.5 0.5
0.0576 0.3598 1
0.0657 0.3453 1
0.1856 0.7894 1
0.0263 0.2948 1
0.0795 1 1
0.0523 1 1
0.1856 0.7894 1
0.0688 0.2765 1
0.054 1 1
0.3788 1 1
0.0658 0.5239 1
0.0322 1 0.5
0.0285 0.325 1
0.3077 0.6314 1
0.0322 1 0.5
0.1856 0.7894 1
0.1856 0.7894 1
0.0527 1 1
0.4502 1 0.5
0.2299 0.3555 0.3555
0.0398 0.3187 1
0.0279 0.5116 1
0.3706 0.768 0.5
0.3077 0.6314 1
0.0679 0.2622 0.9132
0.4527 1 1
0.0322 1 1
0.0288 1 0.5
0.3077 0.6314 1
0.0523 1 1
0.3077 0.6314 1
0.027 0.3801 1
0.0108888 0.531309 0.628565
0.3077 0.6314 1
0.026 1 1
0.1445 0.3454 0.7528
0.2785 0.3707 0.3707
0.393 1 0.5
0.0263 0.8315 1
0.1856 0.7894 1
0.0261 0.3212 0.5537
0.026 1 1
0.0523 1 1
0.4536 1 1
0.3788 1 1
0.0322 0.378 1
0.3077 0.6314 1
0.356 1 1
0.0672 0.2626 0.8746
0.446 1 0.5
0.4564 1 0.5
0.2373 1 1
0.3077 0.6314 1
0.1445 0.3454 0.7529
0.2267 0.3553 0.3553
0.16 1 1
0.0672 0.4131 1
0.2785 0.3707 0.3707
0.3706 0.768 0.5
0.0676 0.2636 0.8753
0.1581 0.4315 1
0.0322 0.4364 1
0.0516 0.292 0.8301
0.5142 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tbru015777AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru015777; Recombinant protein: full-length; Source: T brucei; Nucleoside 2-deoxyribosyltransferase ;

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.5.1360 (Trypanosoma brucei), nucleoside 2-deoxyribosyltransferase
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