Detailed view for Tb927.3.3410

Basic information

TDR Targets ID: 10277
Trypanosoma brucei, aspartyl aminopeptidase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.7004 | Length (AA): 451 | MW (Da): 49327 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02127   Aminopeptidase I zinc metalloprotease (M18)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005773   vacuole  
GO:0008270   zinc ion binding  
GO:0004177   aminopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128020)

Species Accession Gene Product
Arabidopsis thaliana AT5G04710   Zn-dependent exopeptidases superfamily protein
Arabidopsis thaliana AT5G60160   zn-dependent exopeptidases superfamily protein
Babesia bovis BBOV_IV011550   aspartyl aminopeptidase, putative
Brugia malayi Bm1_16690   Aspartyl aminopeptidase
Candida albicans CaO19.9871   similar to vacuolar aminopeptidase
Candida albicans CaO19.2335   similar to vacuolar aminopeptidase
Caenorhabditis elegans CELE_F01F1.9   Protein DNPP-1
Cryptosporidium hominis Chro.30408   hypothetical protein
Cryptosporidium parvum cgd3_3610   possible aspartyl aminopeptidase
Dictyostelium discoideum DDB_G0286149   aspartyl aminopeptidase
Echinococcus granulosus EgrG_001083400   aspartyl aminopeptidase
Entamoeba histolytica EHI_106690   aspartyl aminopeptidase, putative
Entamoeba histolytica EHI_021340   aminopeptidase, putative
Echinococcus multilocularis EmuJ_001083400   aspartyl aminopeptidase
Homo sapiens ENSG00000123992   aspartyl aminopeptidase
Leishmania braziliensis LbrM.29.2330   aspartyl aminopeptidase, putative,metallo-peptidase, Clan MH, Family M20
Leishmania donovani LdBPK_292470.1   aspartyl aminopeptidase, putative
Leishmania infantum LinJ.29.2470   aspartyl aminopeptidase, putative,metallo-peptidase, Clan MH, Family M20
Leishmania major LmjF.29.2360   aspartyl aminopeptidase, putative,metallo-peptidase, Clan MH, Family M20
Leishmania mexicana LmxM.08_29.2360   aspartyl aminopeptidase, putative,metallo-peptidase, Clan MH, Family M20
Loa Loa (eye worm) LOAG_13528   hypothetical protein
Loa Loa (eye worm) LOAG_10667   hypothetical protein
Loa Loa (eye worm) LOAG_04116   aspartyl aminopeptidase
Mycobacterium leprae ML2213c   PROBABLE AMINOPEPTIDASE PEPC
Mus musculus ENSMUSG00000026209   aspartyl aminopeptidase
Mycobacterium tuberculosis Rv0800   Probable aminopeptidase PepC
Neospora caninum NCLIV_006860   hypothetical protein
Oryza sativa 4351851   Os12g0236500
Oryza sativa 4324011   Os01g0967900
Plasmodium berghei PBANKA_0833100   M18 aspartyl aminopeptidase, putative
Plasmodium falciparum PF3D7_0932300   M18 aspartyl aminopeptidase
Plasmodium knowlesi PKNH_0731000   M18 aspartyl aminopeptidase, putative
Plasmodium vivax PVX_087090   M18 aspartyl aminopeptidase, putative
Plasmodium yoelii PY03205   aspartyl aminopeptidase, putative
Saccharomyces cerevisiae YHR113W   Ape4p
Schistosoma japonicum Sjp_0060580   ko:K01267 aspartyl aminopeptidase [EC3.4.11.21], putative
Schistosoma mansoni Smp_159960.2   aspartyl aminopeptidase (M18 family)
Schistosoma mansoni Smp_159960.1   aspartyl aminopeptidase (M18 family)
Schmidtea mediterranea mk4.004830.01   Putative aspartyl aminopeptidase
Trypanosoma brucei gambiense Tbg972.3.3570   aspartyl aminopeptidase, putative,metallo-peptidase, Clan MH, Family M20
Trypanosoma brucei Tb927.3.3410   aspartyl aminopeptidase, putative
Trypanosoma brucei Tb11.v5.0768   aspartyl aminopeptidase, putative
Trypanosoma congolense TcIL3000_0_42510   aspartyl aminopeptidase, putative
Trypanosoma cruzi TcCLB.508153.180   aspartyl aminopeptidase, putative
Trypanosoma cruzi TcCLB.508183.4   metallo-peptidase, Clan MH, Family M20
Theileria parva TP01_1150   aspartyl aminopeptidase, putative
Trichomonas vaginalis TVAG_115460   Clan MH, family M18, aspartyl aminopeptidase-like metallopeptidase
Trichomonas vaginalis TVAG_392410   Clan MH, family M18, aspartyl aminopeptidase-like metallopeptidase
Trichomonas vaginalis TVAG_490070   Clan MH, family M18, aspartyl aminopeptidase-like metallopeptidase

Essentiality

Tb927.3.3410 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu816 Mycobacterium tuberculosis non-essential nmpdr
Tb927.3.3410 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.3410 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.3410 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.3410 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0833100 Plasmodium berghei Dispensable plasmo
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum M18 aspartyl aminopeptidase Compounds References
Homo sapiens aspartyl aminopeptidase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0077 0.2835 0.5
0.0076 0.3769 0.5
0.0073 0.3055 1
0.0606 0.6148 1
0.0608 1 1
0.0087 0.2547 1
0.0228 0.3016 1
0.0167 0.5438 1
0.0071 0.5 0.5
0.0087 0.2578 0.5
0.0463 0.8997 1
0.0163 0.5492 1
0.0087 0.2987 1
0.0087 0.5 0.5
0.0161 0.5661 1
0.0075 0.3032 0.5
0.0074 0.4032 0.7441
0.0087 0.2769 1
0.0213 0.3194 0.3297
0.0087 0.2625 1
0.0087 0.2743 1
0.0087 1 0.5
0.0239 0.8826 1
0.0087 0.2618 1
0.0085 0.2758 1
0.0072 1 0.5
0.0292 0.5286 1
0.0087 0.2509 1
0.0296 0.6078 0.917
0.053 1 1
0.0158 0.6765 0.5
0.0449 0.6146 1
0.0087 1 1
0.0073 0.3055 1
0.0153 0.5036 0.5
0.0527 0.7105 1
0.0151 0.2974 0.438
0.0549 0.726 1
0.0087 0.2513 0.7097
0.0073 0.5396 0.5
0.0087 0.4262 1
0.0291 0.6013 0.9382
0.0228 0.3016 1
0.0072 0.2833 1
0.0074 0.2809 0.5
0.0087 0.4058 0.5895
0.0074 0.4032 0.7441
0.0998 0.6292 1
0.0075 0.4656 0.6388
0.0087 1 1
0.0382 0.776 1
0.0084 0.4089 0.5
0.0146 0.2834 1
0.0087 0.3326 1
0.0087 0.4029 0.7441
0.0146 0.3979 1
0.0079 0.2568 0.628
0.008 1 0.5
0.0076 0.3769 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

13 literature references were collected for this gene.

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Gene identifier Tb927.3.3410 (Trypanosoma brucei), aspartyl aminopeptidase, putative
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