Detailed view for EHI_189420

Basic information

TDR Targets ID: 801129
Entamoeba histolytica, deoxyuridine 5-triphosphate nucleotidohydrolase, putative
Source Database / ID:  AmoebaDB  

pI: 7.7719 | Length (AA): 142 | MW (Da): 15699 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00692   dUTPase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004170   dUTP diphosphatase activity  
GO:0046080   dUTP metabolic process  

Metabolic Pathways

Pyrimidine metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 130 3lqw (A) 2 130 99.99 0 1 2.05655 -0.59

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3LQW:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_131028)

Species Accession Gene Product
Escherichia coli b3640   deoxyuridinetriphosphatase
Entamoeba histolytica EHI_189420   deoxyuridine 5-triphosphate nucleotidohydrolase, putative
Mycobacterium leprae ML1028   PROBABLE DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE DUT (DUTPASE) (DUTP PYROPHOSPHATASE) (DEOXYURIDINE 5'-TRIPHOSPHATASE)
Mycobacterium tuberculosis Rv2697c   Probable deoxyuridine 5'-triphosphate nucleotidohydrolase Dut (dUTPase) (dUTP pyrophosphatase) (deoxyuridine 5'-triphosphatase)
Mycobacterium ulcerans MUL_3335   deoxyuridine 5'-triphosphate nucleotidohydrolase

Essentiality

EHI_189420 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b3640 Escherichia coli essential goodall
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0424 0.2855 0
0.1267 0.3447 0.3447
0.0078 0.3348 0.3337
0.0567 0.338 0.338
0.0272 0.2645 0.3322
0.0175 0.3303 0.323
0.033 0.3069 0
0.0412 0.3415 0.3311
0.048 0.3455 0
0.0191 0.3223 0.3191
0.0409 0.3389 0.3366
0.0245 0.276 0.331
0.0111 0.2527 0.3313
0.1389 0.3459 0.3459
0.0329 0.3398 0
0.0356 0.2521 0.3408
0.0271 0.3106 0.3298
0.0308 0.3349 0.2925
0.1233 0.34 0.3399
0.0473 0.3298 0
0.0356 0.2523 0.3393
0.0245 0.2896 0.3224
0.0286 0.324 0.324
0.0426 0.3441 0.3439
0.1311 0.3421 0.341
0.0245 0.2896 0.3224
0.0333 0.3339 0.3313
0.0335 0.3365 0.3345
0.1145 0.3416 0.3416
0.1186 0.3105 0
0.0273 0.343 0
0.0211 0.3378 0.3286
0.0412 0.3415 0.3311
0.1316 0.3429 0.328
0.0644 0.3384 0.3384
0.1267 0.3447 0.3447
0.0279 0.3437 0
0.0287 0.3379 0.3373
0.0623 0.3404 0.3388
0.1233 0.3456 0.3419
0.0392 0.338 0.332
0.0115 0.3095 0
0.0467 0.3081 0.333
0.1213 0.3435 0.3435
0.0499 0.3416 0.3367
0.1285 0.338 0.3375
0.129 0.3446 0.3407
0.0253 0.2674 0.3144
0.1389 0.3459 0.3459
0.0303 0.3434 0
0.0356 0.2516 0.3393
0.0115 0.3074 0.2486
0.0115 0.3095 0
0.041 0.3223 0.3223
0.0359 0.3149 0.3149
0.0335 0.3365 0.3345
0.0374 0.3399 0.3399
0.1262 0.3441 0.3427
0.0424 0.2855 0
0.0299 0.3287 0.3271
0.1389 0.3459 0.3459
0.0283 0.331 0
0.1316 0.3429 0.328
0.0149 0.3386 0.338
0.0269 0.3287 0.3222
0.0467 0.3081 0.333
0.0616 0.3451 0.345
0.048 0.3455 0
0.0313 0.2938 0.3204
0.03 0.3388 0.3382
0.0107 0.282 0.282
0.0382 0.3006 0
0.042 0.345 0.345
0.0142 0.2583 0.2583
0.0242 0.2879 0.2879
0.0418 0.3414 0.3346
0.0245 0.265 0
0.0418 0.3414 0.3346
0.0348 0.3392 0.3392
0.0113 0.3156 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier EHI_189420 (Entamoeba histolytica), deoxyuridine 5-triphosphate nucleotidohydrolase, putative
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