Detailed view for EHI_057660

Basic information

TDR Targets ID: 799240
Entamoeba histolytica, poly(ADP-ribose) glycohydrolase, putative
Source Database / ID:  AmoebaDB  

pI: 8.0648 | Length (AA): 450 | MW (Da): 50409 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05028   Poly (ADP-ribose) glycohydrolase (PARG)

Gene Ontology

Mouse over links to read term descriptions.
GO:0004649   poly(ADP-ribose) glycohydrolase activity  
GO:0005975   carbohydrate metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 443 4a0d (A) 462 943 25.00 0 1 1.19187 -0.38
22 433 4n9z (A) 485 926 27.00 0 1 1.23366 -0.36

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_128766)

Species Accession Gene Product
Arabidopsis thaliana AT2G31870   poly(ADP-ribose) glycohydrolase 1
Arabidopsis thaliana AT2G31865   poly(ADP-ribose) glycohydrolase 2
Brugia malayi Bm1_31545   Poly
Caenorhabditis elegans CELE_H23L24.5   Protein PME-4
Caenorhabditis elegans CELE_F20C5.1   Protein PME-3, isoform D
Cryptosporidium hominis Chro.80255   poly (ADP-ribose) glycohydrolase
Cryptosporidium parvum cgd8_2160   shares a domain with poly(ADP) ribose glycohydrolases, some protein kinase A anchoring proteins and baculovirus HzNV Orf103, pos
Dictyostelium discoideum DDB_G0277943   poly glycohydrolase
Drosophila melanogaster Dmel_CG2864   Poly(ADP-ribose) glycohydrolase
Echinococcus granulosus EgrG_000459600   polyADP ribose glycohydrolase
Entamoeba histolytica EHI_057660   poly(ADP-ribose) glycohydrolase, putative
Entamoeba histolytica EHI_072630   hypothetical protein
Echinococcus multilocularis EmuJ_000459600   poly(ADP ribose) glycohydrolase
Homo sapiens ENSG00000227345   poly (ADP-ribose) glycohydrolase
Loa Loa (eye worm) LOAG_02521   Poly(ADP-ribose) glycohydrolase
Mus musculus ENSMUSG00000021911   poly (ADP-ribose) glycohydrolase
Neospora caninum NCLIV_019770   hypothetical protein
Oryza sativa 4334754   Os03g0843900
Schistosoma japonicum Sjp_0100110   ko:K07759 poly(ADP-ribose) glycohydrolase [EC3.2.1.143], putative
Schistosoma japonicum Sjp_0307260   ko:K02912 large subunit ribosomal protein L32e, putative
Schistosoma mansoni Smp_142180   poly(ADP-ribose) glycohydrolase
Schmidtea mediterranea mk4.000833.03   60S ribosomal protein L32
Schmidtea mediterranea mk4.017975.02  
Schmidtea mediterranea mk4.002627.03  
Schmidtea mediterranea mk4.017975.01   Putative poly(ADP-ribose) glycohydrolase
Schmidtea mediterranea mk4.002627.04   Putative poly(ADP-ribose) glycohydrolase
Schmidtea mediterranea mk4.002496.00   60S ribosomal protein L32
Trypanosoma brucei gambiense Tbg972.9.7860   poly(ADP-ribose) glycohydrolase, putative
Trypanosoma brucei Tb927.9.12810   poly(ADP-ribose) glycohydrolase, putative
Trypanosoma congolense TcIL3000_9_5330   poly(ADP-ribose) glycohydrolase, putative
Trypanosoma cruzi TcCLB.510659.104   poly(ADP-ribose) glycohydrolase, putative
Trypanosoma cruzi TcCLB.507013.24   poly(ADP-ribose) glycohydrolase, putative
Toxoplasma gondii TGME49_280380   poly(ADP-ribose) glycohydrolase

Essentiality

EHI_057660 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.3760 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.3760 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.3760 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.3760 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_280380 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus Poly(ADP-ribose) glycohydrolase Compounds References
Bos taurus Poly [ADP-ribose] glycohydrolase Compounds References
Homo sapiens poly (ADP-ribose) glycohydrolase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.024 0.3706 1
0.0312 0.3856 1
0.0624 0.7758 1
0.0626 0.3618 1
0.0766 0.2589 1
0.0155 0.5488 1
0.0161 0.5 0.5
0.1207 1 1
0.1304 0.8182 1
0.08 0.4785 1
0.0336 1 1
0.0093 0.5312 1
0.1172 0.8475 1
0.0629 0.498 1
0.0302 1 1
0.0634 0.7347 0.9976
0.1323 0.5523 1
0.0188 1 0.5
0.0629 0.4936 0.5454
0.0771 0.2839 1
0.0332 1 1
0.0785 0.9929 1
0.0457 0.2714 1
0.031 1 1
0.0252 1 0.5
0.0282 0.4659 1
0.0624 0.4372 1
0.1163 0.703 1
0.0816 0.8974 1
0.1059 0.3165 1
0.0625 0.3856 1
0.034 0.4161 1
0.0258 1 1
0.0312 0.3856 1
0.0225 1 1
0.0115 0.3579 0.3507
0.0154 0.2731 1
0.046 0.2829 0.7591
0.1322 0.7909 1
0.024 0.3706 1
0.0332 0.6217 1
0.1295 1 1
0.0203 0.3638 1
0.0337 0.5 0.5
0.0783 0.2556 1
0.077 1 1
0.0479 0.6969 1
0.0781 0.2664 1
0.062 0.7105 1
0.015 1 1
0.0155 0.5479 1
0.1172 0.8475 1
0.08 0.4785 1
0.0947 0.5886 1
0.0786 0.6103 1
0.0774 1 1
0.0973 0.2987 1
0.046 0.2719 1
0.0188 1 1
0.076 0.2526 1
0.0188 1 1
0.0624 0.8611 1
0.0866 0.2775 1
0.0637 0.7051 1
0.0945 0.3846 1
0.0781 0.3799 1
0.0983 1 1
0.0479 0.6973 1
0.0164 0.6293 1
0.0947 0.5886 1
0.0761 0.2772 1
0.034 1 1
0.0322 0.3985 1
0.1187 1 1
0.0154 0.3896 1
0.08 0.4785 1
0.0769 0.2771 1
0.113 1 1
0.0151 0.6254 0.6748
0.0153 0.5018 0.4685
0.0165 0.9413 1
0.062 0.7091 1
0.0624 0.7185 1
0.0765 0.2777 1
0.0158 0.347 0.6586
0.1294 1 1
0.0302 1 1
0.0624 0.8611 1
0.0933 0.2781 1
0.0628 0.7164 1
0.0634 0.7347 0.9976
0.0353 0.3241 1
0.0172 0.4499 1
0.0973 0.2987 1
0.0326 1 1
0.0972 0.2945 1
0.0781 0.5683 1
0.08 0.4785 1
0.0152 0.4241 1
0.0097 0.5312 1
0.0465 0.3518 1
0.0152 0.3617 1
0.0929 1 1
0.0946 1 1
0.096 0.3954 1
0.114 1 1
0.033 1 1
0.0975 0.6055 1
0.1107 1 1
0.0483 1 1
0.0447 0.5145 1
0.0969 0.4028 1
0.1172 0.8475 1
0.0311 0.4031 1
0.0796 0.8101 1
0.0214 0.3661 1
0.0771 1 1
0.0629 0.4936 0.5454
0.0313 0.3661 1
0.0492 1 1
0.0781 0.3799 1
0.0305 0.3649 1
0.0312 0.3856 1
0.0756 0.2525 1
0.034 1 0.5
0.0237 0.912 1
0.079 0.3635 1
0.0492 1 1
0.0316 0.9361 1
0.0424 1 1
0.0767 1 1
0.0947 0.5886 1
0.0776 0.6085 1
0.0775 0.2627 1
0.0986 0.3011 1
0.0932 0.3849 1
0.0787 1 1
0.1121 1 1
0.0524 0.8606 1
0.0949 0.5 0.5
0.0957 0.5992 1
0.0973 0.2987 1
0.0969 0.4028 1
0.0816 0.8974 1
0.0317 0.2872 1
0.0151 0.5 0.5
0.0188 1 1
0.0775 0.2838 1
0.0322 1 1
0.1328 0.3131 1
0.1338 0.5421 1
0.0447 0.5145 1
0.035 0.5 0.5
0.1319 0.7895 1
0.024 0.3706 1
0.0948 1 1
0.0973 0.2987 1
0.0302 0.7979 1
0.0781 0.5683 1
0.0447 0.5145 1
0.0332 0.5716 1
0.0781 0.9874 1
0.0188 0.5768 1
0.0973 0.2987 1
0.0629 0.4936 0.5454
0.0341 1 1
0.0356 0.3851 1
0.0154 1 1
0.1328 0.3131 1
0.0346 0.3703 1
0.0761 0.2772 1
0.0769 1 1
0.1178 0.7033 1
0.0188 0.5768 1
0.0171 1 1
0.0972 0.2964 1
0.0151 0.5 0.5
0.0786 0.5 0.5
0.0629 0.4936 0.5454
0.1305 1 1
0.0783 0.9484 1
0.0935 0.7437 1
0.0329 0.4115 1
0.0764 0.251 1
0.024 0.3706 1
0.1027 1 1
0.0322 1 1
0.0634 0.7347 0.9976
0.0774 1 1
0.0781 0.3799 1
0.0171 0.2747 1
0.1168 0.733 1
0.0781 0.3799 1
0.1151 0.6959 1
0.0190178 0.336767 1
0.0491 0.6091 1
0.1139 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier EHI_057660 (Entamoeba histolytica), poly(ADP-ribose) glycohydrolase, putative
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