Detailed view for Rv2150c

Basic information

TDR Targets ID: 6104
Mycobacterium tuberculosis, Cell division protein FtsZ
Source Database / ID:  Tuberculist 

pI: 4.3073 | Length (AA): 379 | MW (Da): 38756 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00091   Tubulin/FtsZ family, GTPase domain
PF12327   FtsZ family, C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0006184   GTP catabolic process  
GO:0043234   protein complex  
GO:0005737   cytoplasm  
GO:0005525   GTP binding  
GO:0003924   GTPase activity  
GO:0051258   protein polymerization  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 312 4e6e (A) 1 312 80.00 0 1 1.80812 -1.38
6 312 2q1x (B) 6 312 99.99 0 1 1.98943 -1.46
11 193 4u39 (H) 14 196 67.00 0 1 1.41475 -1.8
66 309 5v68 (D) 16 309 93.00 0 1 1.4997 -0.06

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 1RLU:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1RQ2:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1RQ7:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2Q1X:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2Q1Y:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4KWE:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 5V68:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 5ZUE:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_128807)

Species Accession Gene Product
Arabidopsis thaliana AT5G55280   cell division protein ftsZ-like protein 1
Arabidopsis thaliana AT3G52750   plastidial division protein FtsZ2-2
Arabidopsis thaliana AT2G36250   cell division protein FtsZ homolog 2-1
Brugia malayi Bm1_56670   Cell division protein ftsZ
Brugia malayi Bm1_22230   Cell division protein ftsZ
Dictyostelium discoideum DDB_G0277721   mitochondrial cell division protein
Escherichia coli b0095   GTP-binding tubulin-like cell division protein
Mycobacterium leprae ML0917   cell division protein FtsZ
Mycobacterium tuberculosis Rv2150c   Cell division protein FtsZ
Mycobacterium ulcerans MUL_3498   cell division protein FtsZ
Oryza sativa 4338932   Os05g0443800
Oryza sativa 4337316   Os04g0665400
Oryza sativa 4333567   Os03g0646100
Treponema pallidum TP0390   cell division protein FtsZ
Wolbachia endosymbiont of Brugia malayi Wbm0602   cell division protein FtsZ

Essentiality

Rv2150c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2183 this record Mycobacterium tuberculosis essential nmpdr
b0095 Escherichia coli essential goodall
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

Compound Source Reference
ChEMBL23 References
ChEMBL23 References
ChEMBL23 References
ChEMBL23 References
ChEMBL23 References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Staphylococcus aureus Cell division protein ftsZ Compounds References
Bacillus subtilis (strain 168) Cell division protein ftsZ Compounds References
Escherichia coli GTP-binding tubulin-like cell division protein Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0617 1 0.5
0.0305 0.3759 1
0.0461 0.4493 1
0.0311 0.4455 0.8199
0.0532318 1 0.5
0.0301 0.44 1
0.0047 0.2592 0.5
0.0187 0.2569 0.4879
0.0319 1 0.5
0.0126 0.2524 1
0.0192 1 1
0.0723 0.2529 1
0.0546 0.2545 0.4797
0.0302 0.3766 1
0.031 0.4088 1
0.0331 1 0.5
0.0313 1 0.5
0.0843 0.2537 1
0.0693 0.7809 0.8268
0.0279 1 0.5
0.0607 1 0.5
0.0045 0.2537 0.5
0.0428 0.2516 1
0.0614 1 0.5
0.0323 0.7628 0.5
0.0284 0.2518 1
0.0298 0.2599 0.5
0.0613 0.781 1
0.0985 1 0.5
0.0299 0.4334 1
0.0601 1 0.5
0.0632 0.7938 1
0.0295 0.3658 1
0.0535 0.2519 0.5
0.0407 0.2502 0.5
0.0276 1 0.5
0.0054 0.2833 0.5
0.0402 0.2538 1
0.0045 0.3536 0.5
0.0685 0.8016 1
0.0701 0.5699 1
0.031 1 1
0.0054 0.2826 0.5
0.0538 0.2536 1
0.0355 0.6887 0.5
0.0222 0.2501 0.8477
0.0628 1 0.5
0.0639 1 0.5
0.004 0.2973 0.5
0.0296887 0.642957 1
0.0498 1 1
0.0635 1 0.5
0.0402 0.2538 1
0.0356 0.2853 1
0.0973 1 0.5
0.0628 0.3859 1
0.0293 0.362 1
0.0305 0.3759 1
0.0525 0.2545 1
0.0072 0.4058 0.5
0.0304 1 0.5
0.0701 0.8167 1
0.0046 0.2632 0.5
0.0067 0.252 0.5
0.0315 0.4428 1
0.0297 0.4329 1
0.0046 0.3108 1
0.0332 0.3001 0.5
0.0068 0.2651 1
0.0597 1 0.5
0.0678 1 0.5
0.0357 1 0.5
0.0265 0.2509 1
0.0731 1 0.5
0.0355 0.6887 0.5
0.0611 1 0.5
0.0602 1 0.5
0.0623 1 1
0.0848 0.2545 1
0.0695 1 0.5
0.0293 0.4028 1
0.0293 0.3636 1
0.0324 0.3504 0.6738
0.0366 0.3708 1
0.0034 0.3051 1
0.0356 0.2878 1
0.031 0.2617 1
0.0481 0.4689 1
0.065 1 0.5
0.0296887 0.642957 1
0.0066 0.35 0.5
0.0525 0.2545 1
0.0307 0.3663 1
0.0322 0.3679 1
0.0051 0.3883 0.5
0.065 1 0.5
0.0299 0.3586 1
0.0849 0.2532 1
0.0405 0.423 1
0.0296 0.5226 0.5
0.0057 0.393 0.5
0.032 0.4436 1
0.0406 0.2514 0.5
0.0316 0.637 0.6311
0.0293 0.3091 1
0.0546 0.2545 0.4797
0.0605 1 0.5
0.0634 1 1
0.0646 0.4293 1
0.0294 1 0.5
0.0973 1 0.5
0.0546 0.2544 0.7947
0.0357 1 0.5
0.0341 0.3867 1
0.0611 1 0.5
0.0551 0.2529 0.5
0.0719 0.2541 0.5
0.0293 0.3136 0.312
0.034 0.393 1
0.0402 0.2538 1
0.032 0.3794 1
0.0366 0.3708 1
0.0356 0.2853 1
0.034 0.393 1
0.0241221 1 1
0.031 0.4449 1
0.0319 0.2722 1
0.0409 0.2544 1
0.0299 0.3537 1
0.0305 0.5935 1
0.0343 0.6891 1
0.0563 0.2544 1
0.0311 0.4455 0.8199
0.0305 0.4319 1
0.0297 0.2963 1
0.0069 0.3392 1
0.0302 0.2563 1
0.0299 0.3537 1
0.0313 1 0.5
0.0477 1 0.5
0.0679 1 0.5
0.0634 1 1
0.0612 0.2949 1
0.0102 0.3953 0.5
0.0403 0.2536 1
0.0092 0.4079 1
0.0293 0.362 1
0.0293 0.361 1
0.0305 0.3759 1
0.0366 0.3708 1
0.0342 0.4361 1
0.0561 0.2529 0.5
0.0701 0.8361 0.5
0.0074 0.4006 0.5
0.04 0.2514 0.5
0.0603 0.6397 1
0.0294 1 0.5
0.0729 0.2541 1
0.0301 0.341 1
0.0404 0.5671 1
0.0293 0.3616 1
0.0294 1 0.5
0.0069 0.3392 1
0.0597 1 0.5
0.0546 0.2544 0.7947
0.0326 0.4263 1
0.085 0.2537 1
0.0841 0.2551 1
0.0293 1 0.5
0.0026 0.2908 0.5
0.0401 0.2516 0.5
0.0324 1 0.5
0.0043 0.4107 0.6062
0.0532318 1 0.5
0.0602 1 1
0.0973 1 0.5
0.0101 0.3552 0.5
0.0973 1 0.5
0.056 0.2514 1
0.0634 1 1
0.0546 0.2545 0.4797
0.0045 0.2572 0.5
0.0319 0.2722 1
0.0405 0.423 1
0.0342 0.4361 1
0.0305 0.3759 1
0.0331 1 1
0.0619 0.7372 1
0.0684 0.253 1
0.032 0.3794 1
0.032 0.3402 1
0.0299 0.7324 1
0.0083 0.2616 0.6473
0.0402 0.2538 1
0.0316 0.6018 0.5
0.0634 1 1
0.0319 0.2722 1
0.0294 0.361 1
0.0305 0.3759 1
0.0331 1 0.5
0.0301 1 0.5
0.0085 0.3918 0.5
0.0338 0.3808 1
0.0657 0.5426 1

Assayability

Assay information

  • FtsZ inactivation in MTB cells at MIC ChEMBL
  • FtsZ inactivation in MTB cells at MIC
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization at 10 uM
  • ChEMBL
  • Induction of Mycobacterium tuberculosis FtsZ GTPase activity
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization at 40 uM by transmission electron microscopy
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization at 80 uM by transmission electron microscopy
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization at 200 uM by transmission electron microscopy
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization expressed in Escherichia coli by light scattering assay
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization expressed in Escherichia coli at 100 uM by light scattering assay
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization expressed in Escherichia coli BL21 (DE3) using GTP as substrate assessed as disassembly of FtsZ at 40 to 80 uM by transmission electron microscopy
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization expressed in Escherichia coli BL21 (DE3) using GTP as substrate assessed as reduction in aggregate formation at 40 to 80 uM by transmission electron microscopy
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization expressed in Escherichia coli BL21 (DE3) using GTP as substrate assessed as reduction in protofilaments at 40 to 80 uM by transmission electron microscopy
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization expressed in Escherichia coli BL21 (DE3) using GTP as substrate assessed as reduction in density and population of FtsZ polymers at 40 to 80 uM by transmission electron microscopy
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis FtsZ polymerization expressed in Escherichia coli BL21 (DE3) using GTP as substrate by spectrofluorimetric analysis
  • ChEMBL
  • Binding affinity to Mycobacterium tuberculosis FtsZ

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier Rv2150c (Mycobacterium tuberculosis), Cell division protein FtsZ
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