Detailed view for Rv3150

Basic information

TDR Targets ID: 6609
Mycobacterium tuberculosis, Probable NADH dehydrogenase I (chain F) NuoF (NADH-ubiquinone oxidoreductase chain F)

Source Database / ID:  Tuberculist 

pI: 6.1101 | Length (AA): 445 | MW (Da): 48102 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01512   Respiratory-chain NADH dehydrogenase 51 Kd subunit
PF10531   SLBB domain
PF10589   NADH-ubiquinone oxidoreductase-F iron-sulfur binding region

Gene Ontology

Mouse over links to read term descriptions.
GO:0051539   4 iron, 4 sulfur cluster binding  
GO:0016651   oxidoreductase activity, acting on NADH or NADPH  
GO:0008137   NADH dehydrogenase (ubiquinone) activity  
GO:0051287   NAD binding  
GO:0010181   FMN binding  
GO:0055114   oxidation reduction  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 438 3i9v (1) 14 437 52.00 0 1 1.61179 -0.78

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase. hasan
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_127601)

Species Accession Gene Product
Arabidopsis thaliana AT5G08530   NADH dehydrogenase [ubiquinone] flavoprotein 1
Brugia malayi Bm1_14150   NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial precursor
Candida albicans CaO19.11971   potential mitochondrial NADH-Ubiquinone Oxidoreductase Complex I subunit similar to Y.lipolytica NUBM subunit and N. crassa and
Candida albicans CaO19.4495   potential mitochondrial NADH-Ubiquinone Oxidoreductase Complex I subunit similar to Y.lipolytica NUBM subunit and N. crassa and
Caenorhabditis elegans CELE_C09H10.3   Protein NUO-1
Dictyostelium discoideum DDB_G0288875   NADH dehydrogenase ubiquinone flavoprotein 1
Drosophila melanogaster Dmel_CG11423   CG11423 gene product from transcript CG11423-RA
Drosophila melanogaster Dmel_CG9140   CG9140 gene product from transcript CG9140-RA
Escherichia coli b2284   NADH:ubiquinone oxidoreductase, chain F
Echinococcus granulosus EgrG_000366000   NADH dehydrogenase ubiquinone flavoprotein 1
Echinococcus multilocularis EmuJ_000366000   NADH dehydrogenase (ubiquinone) flavoprotein 1
Homo sapiens ENSG00000167792   NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
Leishmania braziliensis LbrM.05.0980   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania donovani LdBPK_050980.1   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania infantum LinJ.05.0980   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania major LmjF.05.0980   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania mexicana LmxM.05.0980   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Loa Loa (eye worm) LOAG_00610   NADH-ubiquinone oxidoreductase 51 kDa subunit
Mus musculus ENSMUSG00000037916   NADH dehydrogenase (ubiquinone) flavoprotein 1
Mycobacterium tuberculosis Rv3150   Probable NADH dehydrogenase I (chain F) NuoF (NADH-ubiquinone oxidoreductase chain F)
Mycobacterium ulcerans MUL_2464   NADH dehydrogenase I subunit F
Oryza sativa 4344095   Os07g0645400
Onchocerca volvulus OVOC3818   NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial homolog
Schistosoma japonicum Sjp_0049230   ko:K03942 NADH dehydrogenase (ubiquinone) flavoprotein 1, putative
Schistosoma mansoni Smp_010710   NADH-ubiquinone oxidoreductase
Schmidtea mediterranea mk4.000692.02   NADH dehydrogenase
Trypanosoma brucei gambiense Tbg972.5.450   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trypanosoma brucei Tb927.5.450   NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
Trypanosoma brucei Tb11.v5.0222   NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial, putative
Trypanosoma congolense TcIL3000_5_70   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trypanosoma cruzi TcCLB.508399.10   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trypanosoma cruzi TcCLB.507869.10   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trichomonas vaginalis TVAG_133030   NADH-ubiquinone oxidoreductase flavoprotein, putative
Trichomonas vaginalis TVAG_188080   NADH-ubiquinone oxidoreductase flavoprotein, putative
Wolbachia endosymbiont of Brugia malayi Wbm0474   NADH dehydrogenase I subunit F

Essentiality

Rv3150 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3202 this record Mycobacterium tuberculosis non-essential nmpdr
Tb927.5.450 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.450 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.450 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.450 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2284 Escherichia coli non-essential goodall
CELE_C09H10.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_C09H10.3 Caenorhabditis elegans larval arrest wormbase
CELE_C09H10.3 Caenorhabditis elegans slow growth wormbase
CELE_C09H10.3 Caenorhabditis elegans sterile wormbase
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa Compounds References
Bos taurus Mitochondrial complex I; NADH oxidoreductase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0316 0.3964 1
0.024 0.3209 0.5035
0.0769 0.4521 0.443
0.234 0.9863 1
0.1477 1 1
0.0331 0.2632 0.8044
0.3309 1 1
0.0078 0.3007 1
0.033 0.3231 0.3859
0.3576 1 1
0.0097 0.371 0.371
0.0336 0.266 0.7027
0.2946 0.3824 1
0.0768 0.4346 0.4061
0.0675 0.3673 0.3462
0.0087 0.2928 1
0.0342 0.6892 0.7462
0.0079 1 0.5
0.0078 0.371 0.371
0.0593 1 1
0.0496 0.689 1
0.3339 0.2726 0.293
0.0683 0.4131 0.4074
0.0496 0.4693 1
0.2073 1 1
0.2175 1 1
0.0956 0.5388 0.5038
0.198 1 1
0.0769 0.4521 0.443
0.0079 0.4008 1
0.0097 1 1
0.032 0.2652 0.2836
0.2073 1 1
0.0189 1 0.5
0.0078 0.2844 1
0.0498 0.3482 1
0.0273 1 0.5
0.0486 0.3455 1
0.0484 0.2831 0.2809
0.0174 0.5264 0.5
0.0097 0.9821 0.5
0.0158 0.346 0.346
0.0338 1 0.5
0.2727 0.9906 1
0.2189 0.3524 1
0.0318 0.4634 1
0.3432 1 1
0.058 0.5115 0
0.2286 0.3525 1
0.1439 1 1
0.0675 0.3584 0.3351
0.017 0.6593 0.5
0.0358 0.4277 1
0.075 1 1
0.2089 1 1
0.0158 0.2773 0.2764
0.0185 1 0.5
0.0402 0.4935 1
0.0237 0.262 0.312
0.0508 0.864 1
0.0239 1 1
0.117 1 0.5
0.0479 0.2878 0.2874
0.0771 0.4111 0.4109
0.0654 1 1
0.0409 0.7374 1
0.237 0.3446 1
0.1973 1 1
0.0315 0.4397 1
0.0151 1 0.5
0.0586 0.2682 0.2607
0.0838 0.5445 0.8542
0.0079 1 0.5
0.0164 0.5067 0.8306
0.1592 1 1
0.0357 0.8153 1
0.0094 1 0.5
0.0663 0.348 0.3418
0.0496 0.2854 0.2819
0.3297 1 1
0.0357 0.8153 1
0.032 0.2652 0.2836
0.032 0.2652 0.2836
0.0759 0.3547 0.3351
0.0244 0.5773 1
0.2766 0.2557 1
0.2283 0.4343 1
0.2889 0.3356 1
0.0243 0.5668 1
0.0322 0.3935 1
0.0158 0.2773 0.2764
0.0838 0.5445 0.8542
0.0081 0.2926 0.5
0.0651 0.4052 0.4012
0.0051 0.5057 0.5
0.0835 0.5158 0.5025
0.0331 0.2632 0.8044
0.016 0.3163 0.3145
0.0479 0.2878 0.2874
0.2802 0.3416 0.9882
0.0496 0.2854 0.2819
0.0097 1 1
0.0434 0.5355 0.6809
0.0498 0.3482 1
0.0252 0.466 0.4967
0.0237 0.8184 1
0.0401 0.4734 0.8736
0.0569 0.8184 1
0.0081 0.3811 1
0.0586 0.3075 0.297
0.2802 0.3416 0.9882
0.0434 0.5355 0.6809
0.0484 0.4371 1
0.0689 1 1
0.0252 0.466 0.4967
0.0167 1 1
0.0675 0.3462 0.3462
0.3309 1 1
0.0243 0.2518 0.9388
0.0254 0.294 1
0.0236 0.5548 0.8358
0.0157 0.3241 0.6597
0.2727 0.9906 1
0.0498 0.3482 1
0.0323 0.3141 1
0.0161 0.3941 0.5
0.0174 0.5264 0.5
0.051 0.6236 1
0.3309 1 1
0.0759 0.3785 0.3765
0.0938 0.5433 0.5082
0.2901 0.4599 1
0.0169 1 0.5
0.0273 0.7192 1
0.0092 0.3608 0.5
0.0249 0.7331 1
0.0345 0.3609 1
0.289 1 1
0.3576 1 1
0.3297 1 1
0.0407 0.4047 0.4866
0.0097 1 0.5
0.0174 0.5264 0.5
0.0675 0.3462 0.3462
0.0572 0.3044 0.294
0.0177 0.5904 0.5
0.058 0.3599 0.3571
0.0479 0.2878 0.2874
0.093 0.4232 0.5619
0.0092 0.4756 0.4756
0.0162 0.485 0
0.058 0.3363 0.3351
0.0419 1 1
0.0097 1 0.5
0.0237 1 1
0.0081 0.3894 1
0.0585 0.4127 1
0.0762 0.4258 0.4217
0.0317 0.2867 1
0.0243 0.2518 0.9388
0.2802 0.3416 0.9882
0.2592 0.6301 1
0.0593 1 1
0.0593 0.5297 0.6973
0.0405 0.3914 1
0.0512 0.6357 1
0.2075 1 1
0.0488 0.6844 1
0.104 0.5726 0.5364
0.0479 0.2878 0.2874
0.0687 0.3955 0.3878
0.0316 0.3964 1
0.0753 0.3746 0.3527
0.0237 0.2991 1
0.0689 0.368 0.368
0.0588 0.3088 0.2982
0.0162 0.2892 0.2886
0.0488 0.6844 1
0.0078 0.2626 0.5
0.0273 1 0.5
0.0163 0.9411 1
0.3309 1 1
0.0851 0.4469 0.4459
0.0489 0.6851 1
0.0078 1 0.5
0.0239 0.3198 1
0.0079 0.2868 1
0.0579 0.2653 0.2577
0.0488 0.6844 1
0.1381 1 1
0.2774 0.319 0.8656
0.0079 0.2706 0.2706
0.0689 0.368 0.368
0.0163 0.9411 1
0.1766 1 1
0.0867 0.4865 0.4865
0.1723 1 1
0.0081 1 1
0.1631 1 0.5
0.0669 0.3324 0.3324
0.2039 1 1
0.041 0.292 0.8676
0.0781 0.4005 0.4005
0.0243 0.5668 1
0.058 0.3599 0.3571
0.0956 0.5388 0.5038
0.076 0.4351 0.407
0.0322 0.3473 0.3473
0.0158 0.7556 1
0.0331 0.2555 1
0.0851 0.4469 0.4459
0.0531 1 1
0.058 0.3363 0.3351
0.0181 0.2679 0.9189
0.0165 1 0.5
0.094 0.5431 0.5431
0.0097 0.3963 1
0.0246 0.51 0
0.1064 0.6024 1
0.0434 0.5355 0.6809
0.3098 1 1
0.0358 0.4277 1
0.0097 1 0.5
0.0698 0.3523 0.3325
0.0679 0.3916 0.3853
0.0252 0.466 0.4967
0.076 0.3871 0.3638
0.0329 0.3129 1
0.0674 0.3905 0.3825
0.0434 0.5355 0.6809
0.2802 0.3416 0.9882
0.0326 1 1
0.0081 0.3811 1
0.162 1 0.5
0.0092 0.371 0.371
0.2802 0.3416 0.9882

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Rv3150 (Mycobacterium tuberculosis), Probable NADH dehydrogenase I (chain F) NuoF (NADH-ubiquinone oxidoreductase chain F)
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