Detailed view for LmjF.27.0500

Basic information

TDR Targets ID: 26284
Leishmania major, calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.9226 | Length (AA): 6164 | MW (Da): 700259 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00648   Calpain family cysteine protease

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0004198   calcium-dependent cysteine-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 26 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 517 1df0 (A) 38 574 23.00 0 1 0.01 0.43
28 298 1zcm (A) 84 351 26.00 0.0000000035 1 0.32 -0.85
606 917 2g8j (A) 39 354 21.00 0 0.85 0.28 -0.36
617 1224 1kfu (L) 34 691 22.00 0 1 0.22 0.34
2246 3113 1tr2 (A) 146 1051 13.00 0.0000062 0.73 0.1 0.75
3 544 3bow (A) 36 599 21.00 0 1 0.13813 0.66
4 301 2p0r (A) 34 337 22.00 0 1 0.244645 -0.35
4 298 2nqa (A) 37 341 24.00 0 1 0.234059 -0.3
29 446 1kfx (L) 75 509 28.00 0 1 0.159113 0.6
599 1069 1qxp (B) 15 516 19.00 0 1 0.125711 0.62
624 912 1ziv (A) 38 332 26.00 0 1 0.261085 -0.38
667 912 1mdw (A) 93 339 28.00 0.000000025 0.93 0.329209 -0.05
1179 1346 4tko (B) 77 244 8.00 0.075 0.01 0.095555 -0.16
1241 1335 3u59 (A) 0 98 15.00 0.62 0.01 0.0997121 -0.02
1385 2088 4zhj (B) 644 1323 13.00 0 0.9 0.0644116 0.96
1709 3031 5t8v (A) 391 1836 10.00 0 0.95 0.254833 0.52
2634 3465 4ady (A) 10 924 10.00 0 0.1 0.0541773 0.7
2714 3297 4f5s (A) 62 583 13.00 0 0.8 0.0659437 0.65
2749 3605 4pjw (A) 141 972 12.00 0 0.98 0.0692331 0.74
3877 5442 5b2o (A) 29 1617 15.00 0 1 0.0232558 1.77
4555 5547 1st6 (A) 7 965 13.00 0 0.95 0.126297 0.76
5136 5579 3r6n (A) 180 623 7.00 0.024 0.01 0.115331 -0.01
5144 5377 4tql (A) 9 236 13.00 0 0.97 0.282162 -1.34
5265 5484 2efl (A) 5 234 11.00 0.11 0.04 0.179991 -0.69
5275 5455 4uos (A) 3 184 9.00 0.012 0.01 0.248664 -1.57
5281 5508 3tnf (B) 204 432 11.00 0.27 0.01 0.122289 -0.03

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_130635)

Species Accession Gene Product
Leishmania braziliensis LbrM.27.0610   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania braziliensis LbrM.27.0620   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania braziliensis LbrM.27.2140   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania braziliensis LbrM.27.0600   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania donovani LdBPK_270510.1   calpain-like cysteine peptidase, putative
Leishmania infantum LinJ.27.0500   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania infantum LinJ.27.2520   cysteine peptidase, Clan CA, family C2, putative
Leishmania infantum LinJ.27.2490   calpain-like cysteine peptidase
Leishmania infantum LinJ.27.0510   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.27.0490   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.27.0500   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.27.0510   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania mexicana LmxM.27.0500   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania mexicana LmxM.27.0490   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania mexicana LmxM.27.0510   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei gambiense Tbg972.11.1180   calpain-like protein, putative
Trypanosoma brucei gambiense Tbg972.11.1190   calpain-like cysteine peptidase, putative,antigen, putative,cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei gambiense Tbg972.11.1170  
Trypanosoma brucei Tb11.v5.0432   calpain-like cysteine peptidase, putative
Trypanosoma brucei Tb927.11.1130   calpain-like cysteine peptidase, putative
Trypanosoma brucei Tb427tmp.11.0001   hypothetical protein
Trypanosoma brucei Tb11.v5.1010   variant surface glycoprotein (VSG), putative
Trypanosoma brucei Tb927.11.1090   calpain-like protein, putative
Trypanosoma brucei Tb11.v5.1011   calpain-like cysteine peptidase, putative
Trypanosoma brucei Tb11.v5.1038   calpain-like cysteine peptidase, putative
Trypanosoma brucei Tb927.11.1100   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei Tb927.11.1110   calpain, putative
Trypanosoma brucei Tb11.v5.0702   calpain-like cysteine peptidase, putative
Trypanosoma congolense TcIL3000.11.1030   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma congolense TcIL3000_0_11260   calpain, putative
Trypanosoma cruzi TcCLB.511445.10   calpain-like cysteine peptidase, putative
Trypanosoma cruzi TcCLB.511441.10   calpain cysteine peptidase, putative
Trypanosoma cruzi TcCLB.509013.10   calpain-like cysteine peptidase, putative
Trypanosoma cruzi TcCLB.445281.9   calpain-like cysteine peptidase, putative
Trypanosoma cruzi TcCLB.506721.30   cysteine peptidase, Clan CA, family C2, putative

Essentiality

LmjF.27.0500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.v4.0001 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.v4.0001 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.v4.0001 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.v4.0001 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.47.0036 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.47.0036 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.47.0036 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.47.0036 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.47.0035 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.47.0035 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.47.0035 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.47.0035 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.57.0008 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.57.0008 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.57.0008 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.57.0008 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania mexicana 337527   561727   585910  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 4174-73-6. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 7179420
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania mexicana 337527   561727   585910  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 4174-73-6. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 7179420
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania mexicana 337527   561727   585910  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 4174-73-6. chemical inhibition with known cysteine peptidase inhibitors leads to reduced enzyme activity in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 7179420
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania amazonensis No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: L-Amino acid esters appear to be hydrolyzed by cysteine peptidase and lead to slow growth of Leishmania sp. in cell assay; . References: 1428504 2235078 2345655
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; . References: 2270108
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania amazonensis 81384   576750  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to reduced enzyme activity in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 8023752
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania major 81384   576750  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to reduced enzyme activity in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 8023752
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) host (GO:0018995) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 81989-95-9. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse) and cure of parasite burden; also provided protection against further infection (partial vaccine); General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 11238649
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 81989-95-9. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse) and cure of parasite burden; also provided protection against further infection (partial vaccine); General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 11238649
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) host (GO:0018995) Leishmania tropica No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 233277-99-1. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse lesions) and reduced lesion size; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15172223
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania tropica No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 233277-99-1. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse lesions) and reduced lesion size; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15172223
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania tropica No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 233277-99-1. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; inhibitors overcome redundancy?; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15172223
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani 0   6693   81384   236103   327961   376038   543486   543487   575389   575390   575391   576750  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 22386-37-4; Drug: 36677-78-8; Drug: 52090-11-6; Drug: 98319-30-3; Drug: 300670-24-0; Drug: 302939-54-4; Drug: 303216-44-6; Drug: 309742-24-3; Drug: 312280-89-0; Drug: 315703-20-9; Drug: 320369-30-0; Drug: 322409-94-9; Drug: 330220-86-5; Drug: 335206-77-4; Drug: 347910-28-5; Drug: 352560-86-2; Drug: 363180-61-4; Drug: 413580-11-7; Drug: 416878-80-3; Drug: 419547-26-5; Drug: 428852-79-3; Drug: 825612-08-6; Drug: 825612-09-7; Drug: 825612-10-0. in silico virtual screening of falcipains lead to bioactive compounds against Leishmania promastigotes; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15588096
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from in-silico analysis (ECO:0000043) Leishmania donovani 0   6693   81384   236103   327961   376038   543486   543487   575389   575390   575391   576750  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 22386-37-4; Drug: 36677-78-8; Drug: 52090-11-6; Drug: 98319-30-3; Drug: 300670-24-0; Drug: 302939-54-4; Drug: 303216-44-6; Drug: 309742-24-3; Drug: 312280-89-0; Drug: 315703-20-9; Drug: 320369-30-0; Drug: 322409-94-9; Drug: 330220-86-5; Drug: 335206-77-4; Drug: 347910-28-5; Drug: 352560-86-2; Drug: 363180-61-4; Drug: 413580-11-7; Drug: 416878-80-3; Drug: 419547-26-5; Drug: 428852-79-3; Drug: 825612-08-6; Drug: 825612-09-7; Drug: 825612-10-0. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15588096
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania donovani 0   6693   81384   236103   327961   376038   543486   543487   575389   575390   575391   576750  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 22386-37-4; Drug: 36677-78-8; Drug: 52090-11-6; Drug: 98319-30-3; Drug: 300670-24-0; Drug: 302939-54-4; Drug: 303216-44-6; Drug: 309742-24-3; Drug: 312280-89-0; Drug: 315703-20-9; Drug: 320369-30-0; Drug: 322409-94-9; Drug: 330220-86-5; Drug: 335206-77-4; Drug: 347910-28-5; Drug: 352560-86-2; Drug: 363180-61-4; Drug: 413580-11-7; Drug: 416878-80-3; Drug: 419547-26-5; Drug: 428852-79-3; Drug: 825612-08-6; Drug: 825612-09-7; Drug: 825612-10-0. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15588096
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 2935-91-3; Drug: 24117-97-3; Drug: 98647-23-5; Drug: 198124-18-4; Drug: 256238-87-6; Drug: 257287-52-8; Drug: 263157-80-8; Drug: 294854-43-6; Drug: 303124-84-7; Drug: 303147-38-8; Drug: 321579-84-4; Drug: 331247-87-1; Drug: 337923-53-2; Drug: 338791-39-2; Drug: 675828-74-7; Drug: 676226-56-5; Drug: 678967-57-2; Drug: 681213-40-1; Drug: 686272-33-3; Drug: 696586-43-3; Drug: 705250-31-3; Drug: 708987-83-1; Drug: 831234-40-3; Drug: 882161-95-7; Drug: 882161-98-0; Drug: 882161-99-1; Drug: 882162-00-7; Drug: 882162-01-8; Drug: 882162-02-9; Drug: 882162-03-0; Drug: 882162-04-1; Drug: 882162-05-2; Drug: 882162-06-3; Drug: 882162-07-4; Drug: 882162-08-5; Drug: 882162-09-6; Drug: 882162-10-9; Drug: 882162-11-0; Drug: 882162-12-1; Drug: 882162-13-2; Drug: 882162-14-3. in silico virtual screening of falcipains lead to bioactive compounds against Leishmania promastigotes; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16509575
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from in-silico analysis (ECO:0000043) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 2935-91-3; Drug: 24117-97-3; Drug: 98647-23-5; Drug: 198124-18-4; Drug: 256238-87-6; Drug: 257287-52-8; Drug: 263157-80-8; Drug: 294854-43-6; Drug: 303124-84-7; Drug: 303147-38-8; Drug: 321579-84-4; Drug: 331247-87-1; Drug: 337923-53-2; Drug: 338791-39-2; Drug: 675828-74-7; Drug: 676226-56-5; Drug: 678967-57-2; Drug: 681213-40-1; Drug: 686272-33-3; Drug: 696586-43-3; Drug: 705250-31-3; Drug: 708987-83-1; Drug: 831234-40-3; Drug: 882161-95-7; Drug: 882161-98-0; Drug: 882161-99-1; Drug: 882162-00-7; Drug: 882162-01-8; Drug: 882162-02-9; Drug: 882162-03-0; Drug: 882162-04-1; Drug: 882162-05-2; Drug: 882162-06-3; Drug: 882162-07-4; Drug: 882162-08-5; Drug: 882162-09-6; Drug: 882162-10-9; Drug: 882162-11-0; Drug: 882162-12-1; Drug: 882162-13-2; Drug: 882162-14-3. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16509575
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 2935-91-3; Drug: 24117-97-3; Drug: 98647-23-5; Drug: 198124-18-4; Drug: 256238-87-6; Drug: 257287-52-8; Drug: 263157-80-8; Drug: 294854-43-6; Drug: 303124-84-7; Drug: 303147-38-8; Drug: 321579-84-4; Drug: 331247-87-1; Drug: 337923-53-2; Drug: 338791-39-2; Drug: 675828-74-7; Drug: 676226-56-5; Drug: 678967-57-2; Drug: 681213-40-1; Drug: 686272-33-3; Drug: 696586-43-3; Drug: 705250-31-3; Drug: 708987-83-1; Drug: 831234-40-3; Drug: 882161-95-7; Drug: 882161-98-0; Drug: 882161-99-1; Drug: 882162-00-7; Drug: 882162-01-8; Drug: 882162-02-9; Drug: 882162-03-0; Drug: 882162-04-1; Drug: 882162-05-2; Drug: 882162-06-3; Drug: 882162-07-4; Drug: 882162-08-5; Drug: 882162-09-6; Drug: 882162-10-9; Drug: 882162-11-0; Drug: 882162-12-1; Drug: 882162-13-2; Drug: 882162-14-3. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16509575
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania major No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 872361-01-8; Drug: 872361-02-9; Drug: 886061-72-9; Drug: 886061-73-0; Drug: 886061-74-1; Drug: 886061-75-2; Drug: 886061-76-3; Drug: 886061-77-4; Drug: 886061-78-5; Drug: 886061-79-6; Drug: 886061-80-9; Drug: 886061-81-0; Drug: 886061-82-1; Drug: 886061-85-4; Drug: 886061-86-5; Drug: 886061-87-6; Drug: 886061-88-7; Drug: 886061-89-8; Drug: 886061-90-1; Drug: 886061-91-2; Drug: 886061-92-3; Drug: 886061-93-4; Drug: 886061-99-0; Drug: 886062-00-6; Drug: 886062-05-1; Drug: 886062-06-2; Drug: 886062-07-3; Drug: 886062-08-4; Drug: 886062-09-5; Drug: 886062-10-8; Drug: 886062-11-9; Drug: 886062-12-0; Drug: 886062-13-1; Drug: 886062-14-2; Drug: 886062-15-3; Drug: 886062-16-4; Drug: 886062-17-5; Drug: 906674-95-1. chemical inhibition with peptidomimetics and Aziridine-2,3-dicarboxylates leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16801424
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania amazonensis 30267  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with calpain inhibitor leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16842979

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

  • Validation: inhibition during in vitro culture
  • annotated by: aaronjr@u.washington.edu.
  • References: 16842979
  • Validation: in vivo inhibition
  • annotated by: aaronjr@u.washington.edu.
  • References: 11238649 15172223

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)(HIV-1) Protein Rev Compounds References
Human immunodeficiency virus 1 Human immunodeficiency virus type 1 REV Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.117 0.567 0.5635
0.012 0.2625 0
0.0496 0.3205 0.5604
0.0134 0.8372 0
0.011 0.258 1
0.0109 0.258 1
0.0936 0.4189 0.5748
0.0516 0.5679 0.5679
0.0574 0.5709 0.5708
0.0561 0.567 0.5642
0.0594 0.2958 0.574
0.024 0.2694 1
0.0313 0.3516 0.5644
0.0225 0.2628 1
0.0608 0.4328 0.6458
0.0554 0.4289 0.5695
0.0569 0.5739 0.5736
0.0486 0.3922 0.5535
0.0464 0.5295 0.5736
0.0109 0.3594 1
0.0505 0.3955 0.5635
0.0499 0.4024 0.4395
0.0552 0.5712 0.5695
0.0471 0.5709 0.5708
0.0502 0.3762 0.6302
0.0618 0.2525 0.4812
0.0592 0.5683 0.5683
0.1166 0.5659 0.5653
0.0063 0.2941 1
0.011 0.2952 1
0.1051 0.5659 0.5653
0.0493 0.3457 0.5756
0.0498 0.5679 0.5679
0.0485 0.5683 0.5679
0.0469 0.5578 0.5508
0.0497 0.5725 0.5725
0.024 0.2684 1
0.0121 0.2877 0.5
0.1158 0.567 0.5635
0.012 0.3371 1
0.0516 0.5679 0.5679
0.0289 0.2769 0.2735
0.052 0.3103 0.5695
0.0436 0.3373 0.6075
0.0557 0.5598 0.5588
0.0372 0.5672 0.5672
0.0372 0.566 0.5635
0.0477 0.5019 0.5709
0.0481 0.5679 0.5679
0.0579 0.5636 0.5635
0.0536 0.573 0.5695
0.0111 0.3238 1
0.0377 0.2795 0.6223
0.0569 0.5739 0.5736
0.011 0.254 1
0.0661 0.2626 0.5171
0.011 0.2715 0.5
0.0592 0.5818 0.5816
0.0522 0.5587 0.5573
0.0504 0.4854 0.637
0.01 0.2577 0.5
0.0592 0.5818 0.5816
0.0108 0.6027 0
0.0536 0.3897 0.6176
0.048 0.5593 0.5552
0.11 0.5659 0.5653
0.0651 0.2872 0.5931
0.1158 0.567 0.5635
0.0553 0.5842 0.5818
0.1043 0.5659 0.5653
0.0375 0.5765 0.5761
0.0119 0.3403 1
0.0567 0.567 0.5642
0.0544 0.5594 0.5708
0.0135 0.2819 1
0.0258 0.5508 0.5508
0.0471 0.573 0.5725
0.0499 0.4024 0.4395
0.1043 0.4189 0.5748
0.0514 0.5178 0.5642
0.0493 0.5735 0.5725
0.0489 0.5051 0.6332
0.0262 0.5653 0.5635
0.0129 0.3 1
0.0608 0.4328 0.6458
0.0521 0.5636 0.5635
0.0135 0.3206 1
0.0468 0.5172 0.5635
0.0486 0.5732 0.5695
0.0251 0.2707 1
0.0289 0.2769 0.2735
0.0535 0.2771 0.5695
0.0471 0.5709 0.5708
0.0491 0.3263 0.5635
0.0494 0.514 0.5736
0.0499 0.4867 0.6373
0.0121 0.313 1
0.0289 0.2769 0.2735
0.0519 0.4808 0.6393
0.0509 0.5492 0.5559
0.0475 0.3812 0.6238
0.0537 0.5387 0.5841
0.0517 0.5089 0.5639
0.0522 0.2855 0.6395
0.0122 0.3104 1
0.0592 0.5818 0.5816
0.0496 0.538 0.6305
0.0119 0.3346 1
0.0563 0.4246 0.5695
0.0546 0.5679 0.5679
0.0482 0.5679 0.5679
0.011 0.3245 0.5
0.0244 0.2704 1
0.0554 0.2932 0.4973
0.1101 0.4189 0.5748
0.0118 0.6794 0
0.0493 0.2726 0.5579
0.0135 0.2819 1
0.0543 0.6053 0.6053
0.0379 0.3309 1
0.0289 0.2769 0.2735
0.0469 0.5683 0.5679
0.0497 0.3823 0.5695
0.0475 0.573 0.5725
0.0481 0.5679 0.5679
0.0472 0.4655 0.5628
0.0135 0.2968 1
0.0379 0.3309 1
0.0372 0.5672 0.5672
0.0493 0.3457 0.5756
0.0499 0.4854 0.6284
0.0469 0.5683 0.5679
0.0525 0.5653 0.5635
0.1043 0.4189 0.5748
0.0544 0.5648 0.5731
0.0994 0.5659 0.5653
0.0135 0.2819 1
0.0119 0.2588 1
0.0231 0.2703 1
0.0507 0.567 0.5642
0.0251 0.2707 1
0.0525 0.5679 0.5679
0.0218 1 0.5
0.0501 0.5561 0.6305
0.0535 0.2771 0.5695
0.012 0.2925 1
0.0505 0.3955 0.5635
0.012 0.2877 0.5
0.0561 0.2845 0.6207
0.049 0.5131 0.5635
0.0645 0.2757 0.5274
0.0243 0.2685 1
0.0505 0.5581 0.6301
0.1166 0.5659 0.5653
0.1101 0.4189 0.5748
0.1043 0.5659 0.5653
0.0632 0.4751 0.5727
0.0496 0.3205 0.5604
0.0475 0.573 0.5725
0.0135 0.2877 0.5
0.0543 0.5501 0.5708
0.0544 0.5594 0.5708
0.0251 0.2707 1
0.0395 0.2705 0.5635
0.0482 0.5695 0.5695
0.0503 0.5801 0.5765
0.0573 0.568 0.5679
0.0135 0.3371 1
0.0510808 0.362382 0.509412
0.05 0.573 0.5725
0.0131 0.3365 1
0.1101 0.4174 0.5737
0.0135 0.2658 1
0.05 0.5699 0.5695
0.0563 0.5709 0.5708
0.0118 0.6794 0
0.0111 0.2802 1
0.011 0.254 1
0.0543 0.6053 0.6053
0.0467 0.2998 0.5508
0.0472 0.4655 0.5628
0.0379 0.3309 1
0.0488 0.3976 0.6312
0.1158 0.5659 0.5653
0.0467 0.2998 0.5508
0.049 0.5131 0.5635
0.0579 0.2603 0.5532
0.0537 0.3952 0.6206
0.0515 0.3926 0.5695
0.0481 0.5679 0.5679
0.0108 0.3005 1
0.0482 0.5735 0.5725
0.0503 0.5801 0.5765
0.0613 0.2605 0.5398
0.0511 0.4762 0.64
0.0498 0.567 0.5642
0.0116 0.2986 1
0.0554 0.3418 0.5695
0.0368 0.5587 0.5508
0.0257 1 1
0.0492 0.567 0.5642
0.0499 0.4867 0.6373
0.0895 0.4221 0.5783
0.0495 0.5864 0.6272
0.0524 0.3499 0.5695
0.0213 0.4015 1
0.0624 0.2875 0.5382
0.055 0.3535 0.5897
0.0116 0.2986 1
0.0109 0.3037 1
0.0478 0.5175 0.5174
0.0469 0.5679 0.5679
0.0468 0.5172 0.5635
0.0489 0.5713 0.5695
0.0554 0.5893 0.5893
0.0488 0.5534 0.6308
0.0556 0.5679 0.5679
0.01 0.2656 0.5
0.0575 0.3182 0.5868
0.0135 0.2877 1
0.0535 0.569 0.569
0.052 0.5711 0.5695
0.0224 0.262 1
0.0554 0.4289 0.5695
0.0119 0.3132 1
0.0543 0.6053 0.6053
0.11 0.5659 0.5653
0.0873199 0.514956 0.510188
0.0489 0.5051 0.6332
0.0481 0.5679 0.5679
0.0624 0.2875 0.5382
0.047 0.5636 0.5635
0.0569 0.3958 0.5495
0.059 0.2673 0.4299
0.0895 0.4221 0.5783
0.0493 0.3457 0.5756
0.0546 0.3531 0.589
0.0569 0.5739 0.5736
0.0063 0.2963 1
0.0063 0.2947 1
0.0499 0.4024 0.4395
0.0579 0.2603 0.5532
0.055 0.2885 0.5774
0.0237 0.2675 1
0.0229 0.2658 1
0.0111 0.2739 0.5
0.0579 0.5706 0.5635
0.0229 0.2644 1
0.0251 0.2707 1
0.0569 0.5709 0.5708
0.0535 0.2771 0.5695
0.0133 0.3202 0.6379
0.0532008 0.496146 0.49497
0.0465 0.5679 0.5679
0.0574 0.5709 0.5708
0.0063 0.2947 1
0.0569 0.3298 0.5609
0.0465 0.5727 0.5708
0.0559 0.5709 0.5708
0.0619 0.3578 0.5966
0.0525 0.567 0.5642
0.0443 0.6065 0.6053
0.0503 0.5365 0.5555
0.024 0.2668 1
0.1101 0.4174 0.5737
0.0499 0.4024 0.4395
0.0467 0.3286 0.5699
0.0247 0.6739 0.5
0.0497 0.4274 0.5755
0.0543 0.3493 0.6053
0.0251 0.2707 1
0.1101 0.4174 0.5737
0.0567 0.567 0.5642
0.0511 0.4762 0.64
0.0563 0.5709 0.5708
0.0514 0.5178 0.5642
0.0485 0.567 0.5642
0.0582 0.3485 0.5627
0.0512 0.5699 0.5695
0.0511 0.4762 0.64
0.0289 0.2769 0.2735
0.0493 0.567 0.5642
0.0563 0.5735 0.5726
0.0567 0.5679 0.5679
0.0135 0.3995 1
0.0557 0.5598 0.5588
0.0504 0.5711 0.5695
0.0258 0.5574 0.5508
0.0619 0.3578 0.5966
0.011 0.385 0.5
0.0516 0.5065 0.6363
0.052 0.3103 0.5695
0.0474 0.2674 0.5508
0.0135 0.3132 1
0.0081 1 1
0.0474 0.2674 0.5508
0.0994 0.5659 0.5653
0.0489 0.4903 0.4886
0.011 0.254 1
0.024 0.2712 1
0.0468 0.5172 0.5635
0.0081 1 1
0.0135 0.3206 1
0.0224 0.2549 1
0.0525 0.567 0.5642
0.1101 0.4174 0.5737
0.0289 0.2769 0.2735
0.012 0.2969 1
0.0526 0.5735 0.5726
0.024 0.2684 1
0.0081 1 1
0.0838 0.5659 0.5653
0.0365 0.9441 1
0.0481 0.4439 0.5508
0.10252 0.514877 0.510188
0.0873199 0.514956 0.510188
0.11 0.5659 0.5653
0.0535 0.567 0.5642
0.0505 0.3955 0.5635
0.0576 0.2621 0.6043
0.0135 0.2764 0.5
0.0573 0.3467 0.5642
0.0518 0.5636 0.5635
0.0497 0.4274 0.5755
0.0135 0.3303 1
0.0251 0.2707 1
0.0594 0.2988 0.5497
0.0228 0.2699 1
0.0379 0.3309 1
0.0231 0.2703 1
0.0114 0.2818 1
0.1109 0.5659 0.5653
0.1051 0.5659 0.5653
0.0511 0.5029 0.5637
0.0838 0.4195 0.5757
0.1166 0.5659 0.5653
0.1043 0.4189 0.5748
0.0229 0.2639 1
0.0579 0.5574 0.5532
0.0543 0.274 0.4426
0.1051 0.5659 0.5653
0.0489 0.4903 0.4886
0.0135 0.3303 1
0.0537 0.5735 0.5726
0.1109 0.5659 0.5653
0.0436 0.3373 0.6075
0.1158 0.5653 0.5635
0.0109 0.3129 0.3129
0.0838 0.4121 0.5682
0.0522 0.5607 0.564
0.0494 0.514 0.5736
0.0225 0.2703 1
0.0126 0.3176 1
0.0543 0.6053 0.6053
0.0504 0.5002 0.5725
0.0545 0.3874 0.6136
0.0289 0.2769 0.2735
0.0233 0.2675 1
0.0505 0.5735 0.5725
0.0645 0.2757 0.5274
0.0494 0.4994 0.555
0.0496 0.538 0.6305
0.0498 0.5679 0.5679
0.0492 0.567 0.5642
0.0493 0.3457 0.5756
0.0347 0.724 1
0.0109 0.3037 1
0.11 0.5659 0.5653
0.0645 0.2757 0.5274
0.0105 0.5 0.5
0.0554 0.5893 0.5893
0.0558 0.5708 0.5708
0.0496 0.5765 0.5765
0.0499 0.4877 0.6405
0.0482 0.5649 0.5567
0.0135 0.2941 1
0.0516 0.5683 0.5644
0.051 0.5622 0.5635
0.0505 0.3955 0.5635
0.0113 0.2878 0
0.0108 0.258 1
0.0232 0.2675 1
0.0454 0.4987 0.612
0.0557 0.293 0.6084
0.0494 0.3056 0.6213
0.0111 0.293 1
0.0512 0.5713 0.5695
0.0498 0.2757 0.5508
0.0574 0.579 0.574
0.0494 0.514 0.5736
0.06 0.3016 0.5697
0.01 0.2577 0.5
0.0521 0.3351 0.5929
0.1109 0.5659 0.5653
0.0505 0.5581 0.6301
0.0464 0.5679 0.5679
0.0489 0.5735 0.5725
0.0467 0.3286 0.5699
0.0494 0.5699 0.5695
0.0063 0.2963 1
0.0109 0.3382 1
0.011 0.254 1
0.0081 1 1
0.0567 0.567 0.5642
0.0554 0.5893 0.5893
0.007 0.2944 1
0.0365 0.9441 1
0.0289 0.2769 0.2735
0.0232 1 0.5
0.0526 0.5673 0.5708
0.0473 0.5679 0.5679
0.0122 0.264 1
0.0838 0.4195 0.5757
0.0313 0.3611 0.5508
0.0571 0.2827 0.5019
0.0499 0.4024 0.4395
0.011 0.3432 1
0.0645 0.2757 0.5274
0.052 0.3155 0.5695
0.1158 0.5659 0.5653

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

12 literature references were collected for this gene.

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User comments

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Gene identifier LmjF.27.0500 (Leishmania major), calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
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