Detailed view for Bm1_37960

Basic information

TDR Targets ID: 240448
Brugia malayi, Ras protein let-60
Source Database / ID:  GenBank

pI: 6.4775 | Length (AA): 183 | MW (Da): 20802 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00071   Ras family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0005622   intracellular  
GO:0005525   GTP binding  
GO:0003924   GTPase activity  
GO:0015031   protein transport  
GO:0007264   small GTPase mediated signal transduction  
GO:0007165   signal transduction  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 166 1plk (A) 1 166 87.00 0 1 1.7772 -0.79
1 167 3con (A) 1 167 70.00 0 1 1.71777 -1
2 179 4dsu (A) 2 179 79.00 0 1 2.00478 -1.61

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127403)

Species Accession Gene Product
Brugia malayi Bm1_46660   Ras-related protein R-Ras2
Brugia malayi Bm1_37960   Ras protein let-60
Candida albicans CaO19.1760   RAS family protein capable of functionally substituting for S. cerevisiae RAS1 (YOR101W)
Candida albicans CaO19.9329   RAS family protein capable of functionally substituting for S. cerevisiae RAS1 (YOR101W)
Caenorhabditis elegans CELE_ZK792.6   Protein LET-60
Caenorhabditis elegans CELE_C44C11.1   Protein RAS-1, isoform A
Dictyostelium discoideum DDB_G0293434   Ras GTPase
Dictyostelium discoideum DDB_G0292996   Ras GTPase
Dictyostelium discoideum DDB_G0281385   Ras GTPase
Dictyostelium discoideum DDB_G0292998   Ras GTPase
Drosophila melanogaster Dmel_CG9375   Ras oncogene at 85D
Drosophila melanogaster Dmel_CG1167   Ras oncogene at 64B
Echinococcus granulosus EgrG_000944700   ras gtpase
Entamoeba histolytica EHI_137700   Ras family GTPase
Entamoeba histolytica EHI_124610   Ras family GTPase
Entamoeba histolytica EHI_124520   ras-1, putative
Echinococcus multilocularis EmuJ_000944700   ras gtpase
Homo sapiens ENSG00000213281   neuroblastoma RAS viral (v-ras) oncogene homolog
Homo sapiens ENSG00000174775   Harvey rat sarcoma viral oncogene homolog
Homo sapiens ENSG00000133703   Kirsten rat sarcoma viral oncogene homolog
Homo sapiens ENSG00000133818   related RAS viral (r-ras) oncogene homolog 2
Loa Loa (eye worm) LOAG_08202   hypothetical protein
Loa Loa (eye worm) LOAG_03928   Ras protein let-60
Mus musculus ENSMUSG00000025499   Harvey rat sarcoma virus oncogene
Mus musculus ENSMUSG00000055723   related RAS viral (r-ras) oncogene homolog 2
Mus musculus ENSMUSG00000027852   neuroblastoma ras oncogene
Mus musculus ENSMUSG00000030265   v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Saccharomyces cerevisiae YNL098C   Ras family GTPase RAS2
Saccharomyces cerevisiae YOR101W   Ras family GTPase RAS1
Schistosoma japonicum Sjp_0216000   ko:K02833 GTPase Hras, putative
Trypanosoma congolense TcIL3000.11.6550   ras-like small GTPase, putative
Trichomonas vaginalis TVAG_396780   ras-dva small GTPase, putative
Trichomonas vaginalis TVAG_190510   GTP-binding protein rit, putative
Trichomonas vaginalis TVAG_533910   rheb, putative
Trichomonas vaginalis TVAG_121390   rap1 and, putative
Trichomonas vaginalis TVAG_001840   dexras1, putative
Trichomonas vaginalis TVAG_408160   ral, putative

Essentiality

Bm1_37960 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_ZK792.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_ZK792.6 Caenorhabditis elegans larval arrest wormbase
CELE_ZK792.6 Caenorhabditis elegans larval lethal wormbase
CELE_ZK792.6 Caenorhabditis elegans slow growth wormbase
CELE_ZK792.6 Caenorhabditis elegans sterile wormbase
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens Harvey rat sarcoma viral oncogene homolog Compounds References
Homo sapiens neuroblastoma RAS viral (v-ras) oncogene homolog Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0535 0.2544 1
0.0231 0.2757 1
0.021 0.2786 0.6249
0.00755273 1 1
0.0317 0.2951 1
0.0188 0.6537 0
0.0226 0.3674 1
0.0458 0.304 0.6753
0.0069 0.7974 1
0.0565 0.3226 0.0611
0.0366 0.3848 0.4186
0.0066 0.4462 1
0.0535 0.2544 1
0.0588 0.5409 0.4841
0.059 0.7822 0.7822
0.0267 0.5123 1
0.0594 0.6616 0.6616
0.0535 0.2544 1
0.0369 0.5012 1
0.0374955 0.423985 1
0.0287 0.347 1
0.0585 0.6649 0.6649
0.0069 0.7974 1
0.0394 0.2768 0.2768
0.0296 0.4995 0.5362
0.0535 0.2544 1
0.036 0.44 1
0.0069 0.7974 1
0.0585 0.6649 0.6649
0.0364 0.5018 1
0.0097 0.2777 0.3164
0.0535 0.2544 1
0.0288 0.3418 1
0.0319 0.4107 1
0.0269 0.7974 1
0.0337 0.2722 0.2678
0.0067 0.6651 1
0.0482 0.5889 1
0.0182 0.4603 0.8075
0.0045 0.4706 0.4436
0.027 0.7974 1
0.0059 0.6645 1
0.0336 0.7974 1
0.0296 0.4995 0.5362
0.036 0.44 1
0.0595 0.668 0.668
0.0313 0.7646 0.7273
0.0591 0.6632 0.6632
0.0069 0.8558 1
0.06 0.893 1
0.0535 0.2544 1
0.0067 0.6651 1
0.0296 0.4995 0.5362
0.0097 0.2777 0.3164
0.0235 0.9767 1
0.0167 0.4481 1
0.0311 0.614 1
0.0579 0.556 0.556
0.0293 0.3511 1
0.0535 0.2544 1
0.06 0.893 1
0.0069 0.7974 1
0.029 0.3413 1
0.0066 0.4462 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Bm1_37960 (Brugia malayi), Ras protein let-60
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