Detailed view for TGME49_201380

Basic information

TDR Targets ID: 263311
Toxoplasma gondii, chorismate synthase, putative

Source Database / ID:  ToxoDB 

pI: 7.7512 | Length (AA): 536 | MW (Da): 58104 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01264   Chorismate synthase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004107   chorismate synthase activity  
GO:0009073   aromatic amino acid family biosynthetic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 324 1qxo (A) 1 277 35.00 0 1 0.6 1.4
1 519 1r53 (A) 1 369 52.00 0 1 0.569084 1.6
2 521 4lj2 (A) 4 353 44.00 0 1 0.603349 2.2
8 324 1qxo (A) 1 277 35.00 0 1 0.623218 1.19

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128304)

Species Accession Gene Product
Arabidopsis thaliana AT1G48850   chorismate synthase
Candida albicans CaO19.3489   chorismate synthase
Candida albicans CaO19.1986   Chorismate synthase
Chlamydia trachomatis CT_368   chorismate synthase
Escherichia coli b2329   chorismate synthase
Mycobacterium leprae ML0516   Chorismate synthase AroF (5-enolpyruvylshikimate-3-phosphate phospholyase).
Mycobacterium tuberculosis Rv2540c   Probable chorismate synthase AroF (5-enolpyruvylshikimate-3-phosphate phospholyase)
Mycobacterium ulcerans MUL_1759   chorismate synthase
Neospora caninum NCLIV_023120   Chorismate synthase (EC 4.2.3.5), related
Oryza sativa 4332286   Os03g0254800
Plasmodium berghei PBANKA_1121900   chorismate synthase, putative
Plasmodium falciparum PF3D7_0623000   chorismate synthase
Plasmodium knowlesi PKNH_1127200   chorismate synthase, putative
Plasmodium vivax PVX_114265   chorismate synthase
Plasmodium yoelii PY04071   chorismate synthase, putative
Saccharomyces cerevisiae YGL148W   bifunctional chorismate synthase/riboflavin reductase [NAD(P)H] ARO2
Toxoplasma gondii TGME49_201380   chorismate synthase, putative

Essentiality

TGME49_201380 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
b2329 Escherichia coli non-essential goodall
PBANKA_1121900 Plasmodium berghei Dispensable plasmo
TGME49_201380 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) Chorismate synthase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.4997 0.7237 1
0.3678 1 1
0.3715 0.4208 0.4678
0.1054 0.3288 1
0.4138 1 1
0.0216 1 0.5
0.1604 0.4869 1
0.5001 0.6671 1
0.324 0.4358 1
0.3715 0.4208 0.4678
0.324 0.4358 1
0.1425 0.3375 1
0.1798 1 1
0.0698 0.2667 1
0.4049 1 1
0.0204 0.6944 1
0.5001 0.6673 1
0.1055 0.2615 1
0.3256 0.4372 1
0.3457 1 1
0.1236 0.4887 1
0.324 0.4358 1
0.1072 0.4132 1
0.0347 1 0.5
0.4265 1 1
0.1057 0.4208 1
0.2378 0.2987 1
0.4997 0.7237 1
0.3008 0.9689 1
0.1062 0.4273 1
0.1246 0.404 1
0.2373 0.2986 1
0.1764 1 1
0.0182 0.3084 1
0.2362 0.3 1
0.0699 0.8805 1
0.4809 1 1
0.4997 0.7237 1
0.0877 0.3512 1
0.4361 1 1
0.2372 0.3439 1
0.0204 0.3559 1
0.3715 0.4208 0.4678
0.0795 0.3231 1
0.4709 1 1
0.0216 0.4649 1
0.1993 0.3846 1
0.4244 0.4276 1
0.0795 0.3231 1
0.2683 0.3596 1
0.3482 0.4602 1
0.0216 0.5 0.5
0.3469 0.8059 1
0.1439 0.3381 1
0.2166 1 1
0.0216 0.3256 0.5
0.218 0.539 1
0.0347 1 0.5
0.1097 0.2734 1
0.2392 0.3483 1
0.1797 0.261 1
0.0795 0.3231 1
0.5344 0.4401 1
0.4809 1 1
0.3663 1 1
0.1079 0.2528 0.7813
0.1991 0.2547 1
0.1061 0.4224 1
0.0884 0.3457 1
0.094 0.2945 1
0.1069 1 1
0.288 0.3905 1
0.1064 0.313 1
0.5898 1 1
0.4831 0.7295 1
0.2899 0.3658 1
0.2643 0.9857 1
0.0192 0.2642 1
0.1604 0.4869 1
0.0216 1 0.5
0.3533 1 1
0.2571 0.3718 1
0.2182 1 1
0.0191 0.47 1
0.3715 0.4208 0.4678
0.3222 1 1
0.0888 1 1
0.0699 0.7081 1
0.1818 0.2665 1
0.2749 0.4911 1
0.0744 0.3514 1
0.5001 0.6671 1
0.407 1 1
0.3031 0.5641 1
0.0663 0.7852 1
0.0698 0.2721 1
0.3715 0.4208 0.4678
0.4997 0.7237 1
0.3715 0.4208 0.4678
0.4385 1 1
0.2168 0.3171 1
0.324 0.4358 1
0.1807 0.4344 1
0.1972 0.3857 1
0.4997 0.7237 1
0.324 0.4358 1
0.218 0.3172 1
0.4445 1 1
0.0216 0.444 0.5
0.3758 1 1
0.0552 0.8898 1
0.3715 0.4208 0.4678
0.5729 0.8248 1
0.272 0.5114 1
0.18 0.4584 1
0.106 0.2602 1
0.4972 0.8659 1
0.4997 0.7237 1
0.107 0.2511 1
0.4138 1 1
0.2182 0.3188 1
0.2314 0.6929 1
0.324 0.4358 1
0.1797 0.261 1
0.0534 0.3131 0.7592
0.4809 1 1
0.18 0.4584 1
0.1435 1 1
0.0174 0.2731 1
0.2998 0.9417 1
0.4049 1 1
0.0216 1 0.5
0.1435 1 1
0.0216 0.536 0.5901
0.1798 1 1
0.4244 0.4276 1
0.2331 0.3449 1
0.286 0.378 1
0.2388 0.3518 1
0.0527 0.3285 0.7944
0.0723 0.3614 1
0.1062 0.4273 1
0.0176 0.434 1
0.3715 0.4208 0.4678
0.1959 0.5528 1
0.1973 0.5837 1
0.0663 0.7852 1
0.4663 1 1
0.3452 0.4187 1
0.2168 0.2771 1
0.1236 0.4887 1
0.2381 0.2773 1
0.0204 0.3559 1
0.1988 1 1
0.1786 0.5428 1
0.0178 0.4339 1
0.0192 0.2642 1
0.3426 1 1
0.0179 0.3155 1
0.3715 0.4208 0.4678
0.3663 1 1
0.29 0.3912 0.9463
0.1829 1 1
0.0351 0.3474 1
0.0705 1 1
0.3715 0.4208 0.4678
0.2166 1 1
0.2188 0.2821 1
0.3173 1 1
0.3415 1 1
0.2331 0.3449 1
0.3296 0.4411 1
0.0204 0.3559 1
0.1614 0.3123 1
0.4809 1 1
0.0175 0.5345 0.5345
0.29 0.3912 0.9463
0.106 0.2602 1
0.1068 0.4301 1
0.2304 0.4753 1
0.107 0.2511 1
0.218 0.3172 1
0.071 1 0.5
0.324 0.4358 1
0.1999 0.2932 1
0.0216 1 0.5
0.0744 0.3514 1
0.3008 0.9689 1
0.0216 0.4649 1
0.4766 0.7953 1
0.106 1 1
0.1999 0.256 1
0.0523 1 0.5
0.2869 0.5022 1
0.1246 0.404 1
0.4596 1 1
0.0682 0.2752 1
0.4871 1 1
0.4997 0.7237 1
0.0216 0.5 0.5
0.2175 0.5313 1
0.2493 0.4382 1
0.0795 0.3231 1
0.4047 1 1
0.3347 1 1
0.2623 1 1
0.3715 0.4208 0.4678
0.199 0.3352 1
0.3715 0.4208 0.4678
0.324 0.4358 1
0.1062 0.4273 1
0.4766 0.7953 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

3 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_201380 (Toxoplasma gondii), chorismate synthase, putative
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