Detailed view for EHI_067860

Basic information

TDR Targets ID: 801890
Entamoeba histolytica, malate dehydrogenase, putative
Source Database / ID:  AmoebaDB  

pI: 5.3717 | Length (AA): 329 | MW (Da): 36108 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00056   lactate/malate dehydrogenase, NAD binding domain
PF02866   lactate/malate dehydrogenase, alpha/beta C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016616   oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor  
GO:0016615   malate dehydrogenase activity  
GO:0016491   oxidoreductase activity  
GO:0003824   catalytic activity  
GO:0055114   oxidation reduction  
GO:0019752   carboxylic acid metabolic process  
GO:0006108   malate metabolic process  
GO:0005975   carbohydrate metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 328 1civ (A) 39 362 36.00 0 1 1.44522 -0.73
13 328 4tvo (A) 4 324 41.00 0 1 1.54679 -1.12
13 328 5mdh (A) 1 326 44.00 0 1 1.54979 -1.01
14 328 4uup (A) 3 327 45.00 0 1 1.56375 -1.08

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_126911)

Species Accession Gene Product
Arabidopsis thaliana AT4G17260   lactate/malate dehydrogenase family protein
Babesia bovis BBOV_IV011270   lactate dehydrogenase
Brugia malayi Bm1_43730   lactate dehydrogenase.
Caenorhabditis elegans CELE_F13D12.2   Protein LDH-1
Cryptosporidium hominis Chro.70062   malate dehydrogenase
Cryptosporidium hominis Chro.70063   lactate dehydrogenase
Cryptosporidium parvum cgd7_480   lactate dehydrogenase, adjacent gene encodes predicted malate dehydrogenase
Cryptosporidium parvum cgd7_470   malate dehydrogenase, adjacent gene encodes predicted lactate dehydrogenase
Drosophila melanogaster Dmel_CG13334   CG13334 gene product from transcript CG13334-RB
Drosophila melanogaster Dmel_CG10160   Ecdysone-inducible gene L3
Echinococcus granulosus EgrG_000467700   L lactate dehydrogenase
Echinococcus granulosus EgrG_000661000   lactate dehydrogenase protein
Echinococcus granulosus EgrG_000608500   lactate dehydrogenase a
Echinococcus granulosus EgrG_000634800   L lactate dehydrogenase B chain
Echinococcus granulosus EgrG_000660800   lactate dehydrogenase a
Entamoeba histolytica EHI_067860   malate dehydrogenase, putative
Echinococcus multilocularis EmuJ_000608500   lactate dehydrogenase a
Echinococcus multilocularis EmuJ_000634800   L lactate dehydrogenase B chain
Echinococcus multilocularis EmuJ_000660800   lactate dehydrogenase a
Echinococcus multilocularis EmuJ_000661000   lactate dehydrogenase a
Echinococcus multilocularis EmuJ_000007100   lactate dehydrogenase protein
Echinococcus multilocularis EmuJ_000467700   L lactate dehydrogenase
Homo sapiens ENSG00000111716   lactate dehydrogenase B
Homo sapiens ENSG00000166800   lactate dehydrogenase A-like 6A
Homo sapiens ENSG00000134333   lactate dehydrogenase A
Homo sapiens ENSG00000166796   lactate dehydrogenase C
Leishmania braziliensis LbrM.20.0020   malate dehydrogenase, putative
Leishmania donovani LdBPK_340160.1   malate dehydrogenase, putative
Leishmania infantum LinJ.34.0160   malate dehydrogenase, putative
Leishmania major LmjF.34.0150   malate dehydrogenase, putative
Leishmania mexicana LmxM.33.0150   malate dehydrogenase, putative
Loa Loa (eye worm) LOAG_12271   hypothetical protein
Mus musculus ENSMUSG00000030246   lactate dehydrogenase B
Mus musculus ENSMUSG00000030851   lactate dehydrogenase C
Mus musculus ENSMUSG00000063229   lactate dehydrogenase A
Neospora caninum NCLIV_011120   Malate dehydrogenase (EC 1.1.1.37), related
Neospora caninum NCLIV_042910   Malate dehydrogenase (EC 1.1.1.37), related
Neospora caninum NCLIV_032330   Malate dehydrogenase (NAD) (EC 1.1.1.37) (Precursor), related
Oryza sativa 4339856   Os06g0104900
Oryza sativa 4328012   Os02g0105400
Plasmodium berghei PBANKA_1117700   malate dehydrogenase, putative
Plasmodium berghei PBANKA_1340100   L-lactate dehydrogenase
Plasmodium falciparum PF3D7_1324900   L-lactate dehydrogenase
Plasmodium falciparum PF3D7_0618500   malate dehydrogenase
Plasmodium knowlesi PKNH_1131900   malate dehydrogenase, putative
Plasmodium knowlesi PKNH_1203900   L-lactate dehydrogenase
Plasmodium vivax PVX_114050   malate dehydrogenase, putative
Plasmodium vivax PVX_116630   lactate dehydrogenase
Plasmodium yoelii PY03376   malate dehydrogenase
Plasmodium yoelii PY03885   l-lactate dehydrogenase
Schistosoma japonicum Sjp_0117410   L-lactate dehydrogenase B chain, putative
Schistosoma japonicum Sjp_0054460   ko:K00016 L-lactate dehydrogenase [EC1.1.1.27], putative
Schistosoma japonicum Sjp_0054470   L-lactate dehydrogenase A chain, putative
Schistosoma japonicum Sjp_0110090   ko:K00016 L-lactate dehydrogenase [EC1.1.1.27], putative
Schistosoma mansoni Smp_038960   L-lactate dehydrogenase
Schistosoma mansoni Smp_038950   malate dehydrogenase
Schmidtea mediterranea mk4.012266.00   L-lactate dehydrogenase
Schmidtea mediterranea mk4.001801.04   L-lactate dehydrogenase
Schmidtea mediterranea mk4.053509.00   L-lactate dehydrogenase
Schmidtea mediterranea mk4.027145.02   L-lactate dehydrogenase
Schmidtea mediterranea mk4.005513.02   L-lactate dehydrogenase
Schmidtea mediterranea mk4.001167.10   L-lactate dehydrogenase
Toxoplasma gondii TGME49_291040   lactate dehydrogenase LDH2
Toxoplasma gondii TGME49_318430   malate dehydrogenase MDH
Toxoplasma gondii TGME49_232350   lactate dehydrogenase LDH1
Theileria parva TP01_1182   L-lactate dehydrogenase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0244   malate dehydrogenase

Essentiality

EHI_067860 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1117700 Plasmodium berghei Slow plasmo
PBANKA_1340100 Plasmodium berghei Essential plasmo
TGME49_232350 Toxoplasma gondii Probably non-essential sidik
TGME49_318430 Toxoplasma gondii Probably non-essential sidik
TGME49_291040 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens lactate dehydrogenase C Compounds References
Homo sapiens lactate dehydrogenase B Compounds References
Plasmodium falciparum malate dehydrogenase Compounds References
Plasmodium falciparum L-lactate dehydrogenase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Thermus thermophilus Malate dehydrogenase 327 aa 43.5% 315 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0585 0.653 1
0.0742 1 1
0.00523329 1 0.5
0.0111 1 1
0.0635 1 1
0.0192 0.4457 1
0.1467 1 1
0.0255 1 1
0.0934 1 1
0.0639 0.5 0.5
0.0105429 1 1
0.0281 1 1
0.0783 1 1
0.0154 1 1
0.0298 0.2816 1
0.04 0.4764 1
0.009 1 1
0.0197 1 1
0.0111 1 1
0.0777 1 0.5
0.0794 1 1
0.0099 1 1
0.0111 1 1
0.0185 0.2871 1
0.0601 0.5179 1
0.0104 0.3056 0.3056
0.0056 1 1
0.0111 1 1
0.017 0.4383 1
0.0411 1 1
0.0183 0.5948 0.6377
0.0597 0.6445 1
0.0099 1 1
0.0707 1 1
0.0473 0.3312 0.5
0.0135 1 1
0.0738 1 1
0.107 1 1
0.0584 1 1
0.0111 1 1
0.0154 1 1
0.029 1 1
0.0596 1 1
0.0868 1 1
0.0413 1 1
0.0616 1 1
0.0089 0.2759 1
0.0155 0.305 1
0.009 1 1
0.0833 0.6076 1
0.0879 0.2756 1
0.0099 1 1
0.0526 1 1
0.0644 1 1
0.0145 1 1
0.114 0.6612 0.7853
0.0425 0.2736 0.658
0.0154 1 1
0.0353 1 1
0.0631 1 1
0.0099 1 1
0.0319 0.5451 1
0.0883 0.2722 1
0.0265 0.6234 1
0.0843 1 1
0.0839 1 1
0.0527 0.5275 1
0.161358 1 1
0.0087 1 1
0.0717 0.8148 1
0.0111 1 1
0.0189 0.3717 0.5
0.045 0.4448 1
0.0093 1 1
0.0485 1 1
0.0919 1 1
0.0093 1 0.5
0.0618 1 1
0.0573 0.5919 1
0.0453 0.7124 1
0.0632 1 1
0.0095 0.2733 1
0.0154 1 1
0.0141 0.4647 0.5
0.269 1 1
0.0449 0.7241 1
0.0111 1 1
0.098 1 1
0.1448 1 1
0.0596 1 1
0.0574 1 1
0.032 0.2952 1
0.00640072 0.46482 0.46482
0.04 0.4764 1
0.0271 1 1
0.0126 0.5984 1
0.0135 0.6383 1
0.0364 1 1
0.0056 0.4605 0.5
0.0879 0.2756 1
0.0529 1 1
0.0177 0.4156 0.5
0.0405 1 1
0.0791 1 1
0.0634 0.3624 1
0.0639 0.5 0.5
0.00522164 1 0.5
0.0616 1 1
0.1939 1 1
0.0134 1 1
0.055 0.646 1
0.0111 1 1
0.04 0.4764 1
0.0539 0.6641 1
0.0188 0.8143 0.5
0.0099 1 1
0.1114 1 1
0.0056 0.7494 0.5
0.0097 0.3315 1
0.0056 1 1
0.0595 1 1
0.0137 0.9354 1
0.1682 1 1
0.0137 0.3251 1
0.1516 1 1
0.0056 1 1
0.0573 0.5919 1
0.114 0.6612 0.7853
0.0832 0.3897 1
0.1412 1 1
0.0514 1 1
0.0216 0.2501 0.7365
0.0099 1 1
0.0099 1 1
0.0453 0.7124 1
0.0557 1 1
0.0152 0.3576 1
0.0418 0.3666 0.5
0.0544 1 1
0.0111 0.2545 0.5
0.157803 1 1
0.0448 0.7102 1
0.0099 1 1
0.0798 1 1
0.0767 1 0.5
0.0729 1 1
0.0638 1 1
0.0111 1 1
0.2209 1 1
0.0919 1 1
0.0612 0.6616 1
0.0099 1 1
0.0559 0.6159 1
0.0639 0.4797 1
0.0489 1 1
0.157803 1 1
0.0646 0.7726 1
0.1689 1 1
0.1065 1 1
0.054 0.6655 1
0.0152 1 1
0.1033 1 1
0.0154 0.429 1
0.0154 1 1
0.0153 1 1
0.0624 0.4508 1
0.0631 1 1
0.0341 1 1
0.0649 1 1
0.0111 1 1
0.0111 1 1
0.0099 1 1
0.0549 0.3471 1
0.0736 1 1
0.0053 1 0.5
0.0506 0.8505 0.8505
0.0056 1 1
0.0056 1 1
0.0056 1 1
0.0099 1 1
0.0111 1 1
0.0504 1 1
0.0833 0.6076 1
0.0573 0.3424 0.5
0.0596 1 1
0.0247 1 1
0.0154 1 1
0.0266 0.3061 1
0.063 0.4772 1
0.0099 1 1
0.0549 0.3471 1
0.073 1 1
0.0111 1 1
0.0056 1 1
0.0883 0.2722 1
0.0601 1 1
0.0346 1 1
0.2312 1 1
0.0463 1 1
0.0463 1 1
0.0108965 1 0.5
0.0098 0.3355 1
0.0099 1 1
0.1103 1 1
0.0712 0.5296 1
0.1333 1 1
0.0099 1 1
0.1043 1 1
0.0573 0.5919 1
0.0322 1 1
0.0169 1 1
0.0529 1 1
0.0154 1 1
0.0819 1 1
0.04 0.4764 1
0.0056 1 1
0.014 0.5495 1
0.014 0.5495 1
0.0111 1 1
0.0099 1 1
0.0832 0.3897 1
0.0099 1 1
0.0717 0.6889 1
0.0812 0.9737 1
0.0494 0.4942 0.41
0.0099 1 1
0.009 1 1
0.0573 0.5919 1
0.0459 1 1
0.0839 1 1
0.016 1 0.5
0.195 1 1
0.0453 0.7124 1
0.0099 1 1
0.147 1 1
0.0154 1 1
0.0624 0.4508 1
0.0099 1 1
0.0339 1 1
0.064 1 1
0.0089 1 1
0.0192 0.4457 1
0.0097 0.3315 1
0.0341 1 1
0.0081 0.4562 1
0.0293 0.3063 1
0.0053 0.6345 0.6345
0.0056 1 0.5
0.1163 1 1
0.0134 0.4209 1
0.0789 1 1
0.0403 0.7904 1
0.0046 0.9126 1
0.0091 1 1
0.0099 1 1
0.0874 1 1
0.0752 1 1
0.009 1 1
0.0889 1 1
0.0111 1 1
0.0745 1 1
0.044 0.3132 1
0.0683 1 1
0.0266 0.3061 1
0.0453 0.7124 1
0.0111 1 1
0.0793 1 1
0.1794 1 1
0.0609 0.6484 1
0.0111 1 1
0.0628 0.3888 1
0.0361 1 1
0.1114 1 1
0.0183 0.5948 0.6377
0.0315 1 1
0.027 1 1
0.0266 0.3061 1
0.1326 1 1
0.0099 1 1
0.0639 0.4797 1
0.0053 1 0.5
0.0417 0.3312 0.5
0.0049 0.3011 0.3011
0.0111 1 1
0.0134 0.4209 1
0.053 1 1
0.0281 1 1
0.0103 1 1
0.027 1 1
0.1103 1 1
0.00518542 1 0.5
0.0185 1 1
0.0266 0.3061 1
0.0111 1 1
0.0559 0.6159 1
0.0468 1 1
0.0099 1 1
0.0111 1 1
0.0107169 1 0.5
0.1516 1 1
0.0099 1 1
0.0573 0.5919 1
0.0099 1 1
0.0778 1 1
0.0186 1 1
0.0099 1 1
0.1794 1 1
0.0833 0.6076 1
0.0449 0.7241 1
0.063 0.4772 1
0.0339 1 1
0.0095 1 1
0.00536163 1 0.5
0.0629 0.5011 1
0.098 1 1
0.0587 0.3508 1
0.1477 1 1
0.054 0.6655 1
0.0579 1 1
0.0056 1 1
0.0111 1 0.5
0.0833 0.6076 1
0.0298 0.2816 1
0.0552 1 1
0.1366 1 1
0.0281 1 1
0.1056 1 1
0.0833 0.6076 1
0.0183 1 1
0.0346 1 1
0.0833 0.6076 1
0.0099 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier EHI_067860 (Entamoeba histolytica), malate dehydrogenase, putative
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