TDR Targets

Detailed view for TcCLB.508207.230

Basic information

TDR Targets ID: 43300
Trypanosoma cruzi, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative
Source Database / ID: TriTrypDB GeneDB

pI: 6.5801 | Length (AA): 705 | MW (Da): 78681 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Network

Pfam domains

PF00300 Histidine phosphatase superfamily (branch 1)
PF01591 6-phosphofructo-2-kinase
PF12796 Ankyrin repeats (3 copies)

Gene Ontology

Mouse over links to read term descriptions.

GO:0006003 fructose 2,6-bisphosphate metabolic process
GO:0003873 6-phosphofructo-2-kinase activity
GO:0003824 catalytic activity
GO:0005524 ATP binding
GO:0005515 protein binding
GO:0006000 fructose metabolic process

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 15 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg	Target End	Template	Template Beg	Template End	Identity	Evalue	Model Score	MPQS	zDope
68	242	2f8y (A)	1923	2110	22.00	0	1	0.46	-0.2
124	214	1n0q (A)	2	92	45.00	0	1	0.88	-2.07
234	686	2axn (A)	13	454	27.00	0	1	0.99	-0.84
251	702	1bif ()	37	468	26.00	0	1	1	-1.1
27	207	4k5c (A)	18	177	26.00	0.000000000025	1	0.461238	0.15
73	207	1n0r (A)	1	118	36.00	0	1	0.567989	-0.67
74	207	1awc (B)	38	154	31.00	0.00000011	1	0.497571	-0.48
78	222	4gmr (A)	8	168	36.00	0	1	0.569174	-0.24
81	213	2pnn (A)	120	271	29.00	0.97	1	0.340652	-0.1
124	209	2xen (A)	14	99	37.00	0.000000000089	1	0.745986	-1.62
130	210	5hry (G)	41	122	57.00	0	1	0.902894	-1.36
140	178	2rfm (A)	147	185	36.00	0	0.92	0.466319	0.19
230	684	5ak0 (A)	4	445	18.00	0	1	0.76739	0.47
246	403	4rfv (A)	441	611	14.00	0	0.74	0.348113	-0.52
457	524	3f2i (A)	2	68	22.00	0	0.07	0.187454	0.52

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent	Ranking	Stage	Dataset
Upper 80-100% percentile		metacyclic.	Smircich P

Upregulation Percent	Ranking	Stage	Dataset
Upper 60-80% percentile		epimastigote.	Smircich P

Show/Hide expression data references

Smircich P

Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_147018)

Species	Accession	Gene Product
Leishmania braziliensis	LbrM.26.0340	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-1-like protein
Leishmania donovani	LdBPK_260300.1	6-phosphofructo-2-kinase/fructose-2,6-biphospha ta se1-like protein
Leishmania infantum	LinJ.26.0300	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-1-like protein
Leishmania major	LmjF.26.0310	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-1-like protein
Leishmania mexicana	LmxM.26.0310	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-1-like protein
Trypanosoma brucei gambiense	Tbg972.7.1620	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative
Trypanosoma brucei	Tb927.7.1610	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative
Trypanosoma congolense	TcIL3000_7_1160	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative
Trypanosoma cruzi	TcCLB.508207.230	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative
Trypanosoma cruzi	TcCLB.509509.30	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative

Essentiality

TcCLB.508207.230 has one or more orthologs with essentiality data

Gene/Ortholog	Organism	Phenotype	Source Study
Tb927.7.1610	Trypanosoma brucei	no significant loss or gain of fitness in bloodstream forms (3 days)	alsford
Tb927.7.1610	Trypanosoma brucei	significant gain of fitness in bloodstream forms (6 days)	alsford
Tb927.7.1610	Trypanosoma brucei	no significant loss or gain of fitness in procyclic forms	alsford
Tb927.7.1610	Trypanosoma brucei	significant gain of fitness in differentiation of procyclic to bloodstream forms	alsford

Show/Hide essentiality data references

blattner

Systematic mutagenesis of the E. coli (MG1655) genome

J Bacteriol 2004, 186:4921-4930

shigen

Profiling of E. coli Chromosome (PEC)

National Institute of Genetics, Japan

gerdes

Experimental determination and system-level analysis of essential genes in E. coli MG1655

Gerdes et al., J Bacteriol. 2003 185:5673-84

yeastgenome

Systematic deletion of yeast genes

Saccharomyces Genome Database

plasmo

Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes.

Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

wormbase

C. elegans RNAi experiments

WormBase web site, http://www.wormbase.org, release WS170

goodall

The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis)

Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

alsford

High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome

Genome Res 2011, 21:915-924

keio

Systematic single-gene knock-out mutants of E. coli K12

The Keio Collection

neb

C. elegans RNAi phenotypes

Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

nmpdr

Genome-scale essentiality datasets from published studies (M. tuberculosis)

National Microbial Pathogen Data Resource

sidik

A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes.

Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets

Non orthologous druggable targets

By sequence similarity to non orthologous druggable targets

No additional associated druggable targets

Ranking Plot

Putative Drugs List

Raw	Global	Species
0.0909	0.3716	0
0.2117	0.4209	0.4169
0.0185	0.3536	0.1841
0.0355	0.4098	0
0.0942	0.4137	0
0.0821	0.4214	0.4212
0.0822	0.3724	0.4233
0.1876	0.4213	0.4169
0.2503	0.4225	0.4225
0.1382	0.4243	0.4232
0.1452	0.423	0.423
0.1369	0.4164	0.4099
0.1699	0.4199	0.4183
0.0912	0.3889	0
0.0888	0.4155	0.4149
0.1131	0.4169	0.4169
0.0113	0.2983	0.2954
0.1554	0.4185	0.4184
0.0976	0.4078	0.3769
0.1948	0.4106	0
0.1028	0.3392	0
0.1197	0.4254	0.4239
0.1699	0.4199	0.4183
0.1013	0.4169	0.4169
0.0026	0.3799	0
0.0628	0.4183	0.4183
0.1725	0.4169	0.4169
0.0162	0.4018	0.3967
0.3335	0.4247	0.4244
0.0152	0.2727	0.0242
0.0467	0.3303	0.4193
0.1381	0.4232	0.4232
0.1228	0.4244	0.424
0.0192	0.4092	0.4058
0.078	0.4198	0.4198
0.1051	0.4169	0.4169
0.0985	0.4191	0.4184
0.1024	0.4217	0.4169
0.1053	0.4186	0.4186
0.1158	0.422	0.4174
0.1945	0.4169	0.4169
0.1158	0.422	0.4174
0.2378	0.4021	0.3997
0.1669	0.419	0.419
0.1495	0.3039	0.4109
0.1349	0.4169	0.4169
0.1827	0.4169	0.4169
0.1943	0.3834	0.3641
0.3335	0.4247	0.4244
0.1333	0.4252	0.4252
0.0586	0.4032	0
0.0731	0.4191	0.4191
0.0495	0.306	0.4169
0.1675	0.4148	0.4148
0.2075	0.4254	0.4254
0.1595	0.4178	0.4169
0.3335	0.4247	0.4244
0.019	0.4138	0.4016
0.1436	0.4218	0.4202
0.0101	0.4169	0.4169
0.0849	0.4233	0.4217
0.2024	0.4251	0.4251
0.1627	0.4234	0.4169
0.2306	0.4245	0.4239
0.0108	0.41	0
0.0196	0.2956	0.4178
0.1228	0.4169	0.4169
0.0836	0.4238	0.4238
0.0836	0.3404	0
0.0527	0.4169	0.4169
0.0189	0.4081	0.4014
0.0876	0.3856	0
0.0463	0.4098	0.3715
0.0796	0.3024	0.4169
0.0713	0.4169	0.4169
0.1061	0.4169	0.4169
0.1131	0.4169	0.4169
0.1673	0.4228	0.4228
0.1193	0.3788	0
0.2154	0.4067	0.3773
0.038	0.4211	0.4169
0.1873	0.424	0.424
0.1614	0.3953	0
0.0202	0.3764	0.3764
0.0653	0.4107	0
0.0494	0.415	0.415
0.2197	0.4122	0.4122
0.218	0.4217	0.4215
0.2149	0.4169	0.4169
0.1634	0.4236	0.4233
0.1135	0.4213	0.4169
0.1787	0.3995	0
0.0446	0.4165	0
0.1081	0.4219	0.4169
0.1909	0.4251	0.4248
0.1578	0.3928	0
0.0825	0.4152	0
0.1932	0.4215	0.4212
0.0574	0.419	0.4169
0.1516	0.2895	0.4217
0.0614	0.3217	0
0.1854	0.4169	0.4169
0.0049	0.295	0.295
0.1765	0.3889	0.2697
0.064	0.3268	0
0.1557	0.4169	0.4169
0.1873	0.424	0.424
0.077	0.4077	0.4077
0.2503	0.4225	0.4225
0.3335	0.4247	0.4244
0.1816	0.3044	0
0.0888	0.4155	0.4149
0.0821	0.4214	0.4212
0.1061	0.4169	0.4169
0.2639	0.4229	0.4169
0.0467	0.3303	0.4193
0.1875	0.2724	0.2724
0.2011	0.4243	0.4243
0.1019	0.4227	0.4208
0.1253	0.4231	0.4231
0.1618	0.4254	0.4244
0.0867	0.2688	0.4183
0.1228	0.4169	0.4169
0.1521	0.42	0.4169
0.1467	0.4176	0.4169
0.3604	0.4256	0.4169
0.1377	0.4249	0.4249
0.0128	0.3894	0.3646
0.0704	0.3661	0
0.2036	0.32	0
0.0931	0.4253	0.4252
0.1561	0.3895	0
0.1835	0.4051	0
0.066	0.4122	0.4122
0.1382	0.4243	0.4232
0.1513	0.3941	0
0.1399	0.3184	0
0.0796	0.3024	0.4169
0.1557	0.4169	0.4169
0.3335	0.4247	0.4244
0.0826	0.4255	0.4169
0.0814	0.3727	0
0.1268	0.402	0
0.0821	0.4214	0.4212
0.1382	0.32	0
0.0695	0.398	0.398
0.2327	0.4248	0.4248
0.1631	0.3706	0
0.0183	0.3482	0.3473
0.1932	0.4215	0.4212
0.0976	0.4078	0.3769
0.129	0.4216	0.4169
0.0753	0.424	0.4169
0.0975	0.4224	0.4224
0.0891	0.3121	0
0.0789	0.4114	0.4
0.0127	0.4229	0.4169
0.185	0.4169	0.4169
0.1733	0.4233	0.4169
0.0059	0.2657	0.2657
0.0789	0.4114	0.4
0.1797	0.4203	0.4203
0.0796	0.3024	0.4169
0.184	0.2598	0
0.0113	0.2983	0.2954
0.1932	0.4215	0.4212
0.0459	0.2815	0
0.1945	0.4169	0.4169
0.1948	0.4106	0
0.1902	0.4048	0.3972
0.1516	0.2895	0.4217
0.1812	0.4169	0.4169
0.0633	0.2644	0.4212
0.1381	0.4232	0.4232
0.1723	0.4241	0.4239
0.2306	0.4245	0.4239
0.2572	0.4169	0.4169
0.0195	0.3692	0.3692
0.0905	0.3883	0
0.153	0.3861	0
0.0909	0.3716	0
0.0408	0.3897	0
0.0843	0.4169	0.4169
0.0807	0.389	0
0.0738	0.3086	0.3086
0.1943	0.3834	0.3641
0.0106	0.3407	0.3407
0.099	0.4219	0.4214
0.0888	0.4155	0.4149
0.0938	0.3966	0.3966
0.2462	0.4256	0.4255
0.0456	0.4242	0.4169
0.1797	0.4203	0.4203
0.3877	0.279	0.4189
0.1436	0.4218	0.4202
0.1685	0.4152	0.4152
0.1838	0.3262	0.3262
0.0129	0.3777	0
0.0177	0.4138	0
0.1387	0.3347	0
0.1382	0.4243	0.4232
0.0447	0.4144	0.4027
0.153	0.3861	0
0.2306	0.4245	0.4239
0.0698	0.419	0.417
0.0698	0.4169	0.4169
0.0439	0.2611	0.2611
0.2462	0.4256	0.4255
0.0527	0.4169	0.4169
0.3877	0.279	0.4189
0.1989	0.4201	0.4169
0.0821	0.4214	0.4212
0.1699	0.4199	0.4183
0.1649	0.4242	0.4169
0.3335	0.4247	0.4244
0.1932	0.4215	0.4212
0.2622	0.4215	0.4169
0.3335	0.4247	0.4244
0.114	0.2899	0
0.0839	0.395	0
0.0465	0.2963	0
0.167	0.4233	0.4231
0.1052	0.4229	0.4227
0.1183	0.4186	0.4186
0.0976	0.4078	0.3769
0.0609	0.4153	0.4123
0.0912	0.4179	0.4221
0.1495	0.3039	0.4109
0.028	0.4247	0.4247
0.1173	0.4147	0.4147
0.1699	0.4199	0.4183
0.0527	0.4154	0
0.0134	0.2985	0.4169
0.1158	0.422	0.4174
0.1675	0.422	0.4169
0.0753	0.424	0.4169
0.005	0.3586	0.2941
0.1673	0.4228	0.4228
0.1945	0.4169	0.4169
0.3335	0.4247	0.4244
0.0278	0.2625	0.3759
0.1467	0.4176	0.4169
0.153	0.3861	0
0.0414	0.4151	0.4132
0.3335	0.4247	0.4244
0.2378	0.4021	0.3997
0.1382	0.32	0
0.1495	0.3039	0.4109
0.0644	0.4185	0.4185
0.1436	0.4218	0.4202
0.0299	0.4119	0
0.2024	0.4251	0.4251
0.2012	0.4234	0.4209
0.3709	0.4197	0.4169
0.1932	0.4215	0.4212
0.0985	0.4191	0.4184
0.129	0.4216	0.4169
0.0938	0.3966	0.3966
0.1943	0.3834	0.3641
0.1873	0.424	0.424
0.1377	0.4249	0.4249
0.0789	0.4114	0.4
0.099	0.4219	0.4214
0.2096	0.4018	0
0.1223	0.3685	0
0.0155	0.395	0.395
0.1187	0.4216	0.4169
0.1797	0.4203	0.4203
0.0912	0.4179	0.4221
0.0985	0.4219	0.4219
0.0463	0.4098	0.3715
0.1675	0.4148	0.4148
0.0822	0.3724	0.4233
0.124	0.425	0.4169
0.0867	0.2688	0.4183
0.0185	0.4081	0.4015
0.1773	0.4244	0.4236
0.0642	0.3298	0
0.0807	0.3722	0
0.1173	0.4147	0.4147
0.3335	0.4247	0.4244
0.0299	0.4119	0
0.0891	0.4241	0.4169
0.1495	0.3039	0.4109
0.1838	0.3262	0.3262
0.1019	0.4227	0.4208
0.2011	0.4243	0.4243
0.1773	0.4244	0.4236
0.159	0.4208	0.42
0.2024	0.4251	0.4251
0.1524	0.4248	0.4238
0.0796	0.3024	0.4169
0.0583	0.4169	0.4169
0.1268	0.402	0
0.1854	0.4169	0.4169
0.1052	0.4229	0.4227
0.1399	0.3184	0
0.1228	0.4244	0.424
0.1627	0.4234	0.4169
0.1932	0.4215	0.4212
0.0105	0.3301	0.3301
0.0912	0.4179	0.4221
0.2552	0.3537	0
0.0138	0.4029	0
0.0821	0.4214	0.4212
0.0891	0.4241	0.4169
0.3335	0.4247	0.4244
0.049	0.4122	0.4122
0.1495	0.3039	0.4109
0.1183	0.4186	0.4186
0.0867	0.2688	0.4183
0.0912	0.4179	0.4221
0.1878	0.4246	0.4245
0.0681	0.4126	0
0.1878	0.4246	0.4245
0.218	0.4217	0.4215
0.124	0.425	0.4169
0.3335	0.4247	0.4244
0.0127	0.4229	0.4169
0.1561	0.3895	0
0.1574	0.4169	0.4169
0.1254	0.4259	0.4259
0.0129	0.3631	0
0.0025	0.3092	0.1502
0.0975	0.4224	0.4224
0.3877	0.279	0.4189
0.137	0.4207	0.419
0.2462	0.4256	0.4255
0.0888	0.4155	0.4149
0.3335	0.4247	0.4244
0.0971	0.4141	0
0.0184	0.4041	0
0.2002	0.4253	0.4253
0.1377	0.4249	0.4249
0.1197	0.4254	0.4239
0.1838	0.3262	0.3262
0.0337	0.3827	0.2448
0.3335	0.4247	0.4244
0.1304	0.4149	0
0.2572	0.4169	0.4169
0.1943	0.3834	0.3641
0.1762	0.2805	0
0.0989	0.4215	0.4169
0.0867	0.2688	0.4183
0.1699	0.4199	0.4183
0.3303	0.42	0.4169
0.3335	0.4247	0.4244
0.1773	0.4244	0.4236
0.218	0.4217	0.4215
0.1873	0.424	0.424
0.1417	0.4183	0.418
0.0678	0.4124	0.4111
0.0274	0.3673	0
0.0704	0.3661	0
0.1013	0.4243	0.4231
0.0502	0.4074	0.4074
0.0698	0.4096	0.3888
0.1774	0.4055	0
0.1649	0.4242	0.4169
0.184	0.2598	0
0.1875	0.2724	0.2724

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

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Gene identifier	TcCLB.508207.230 (Trypanosoma cruzi), 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative

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